We narrowed to 81,735 results for: TRI
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Plasmid#132665PurposepyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.DepositorInsertUpstream & Downstream pyrE deletion region
UseE. coli - c. difficile shuttle vectorAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHY23-phage-ubc-nls-ha-stdMCP-tdTomato
Plasmid#164044PurposeExpression of stdMCP-tdTomato in mammalian cellsDepositorInsertstdMCP-tdTomato
UseLentiviralExpressionMammalianPromoterhuman ubiquitin C (UBC)Available SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
PlayBack-CMV-EcoR1
Plasmid#203305PurposeFor expression of eukaryotic genes of interest with CMV promoter with EcoRI as a PlayBack Restriction EnzymeDepositorTypeEmpty backboneExpressionMammalianAvailable SinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
betaRBPIG
Plasmid#16422DepositorInsertloxP flanked RFP and blasticidin-resistance plus puromycin-acetyltransferase IRES GFP
ExpressionMammalianAvailable SinceApril 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
Akap1(31)-FRB-iRFP
Plasmid#139315Purposemitochondrial-resident FRB for chemically induced dimerizationDepositorInsertMus musculus Akap1(1-31) M16L:MTOR(2021-2113):iRFP
ExpressionMammalianMutationM16LAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEED-mCherry
Plasmid#219657PurposePermits GoldenGate (BsmBI) assembly of donor plasmids to knock-in mCherry using the SEED/Harvest methodDepositorInsertsfGFP SEED Cassette
Available SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVPRT-tTR-KRAB
Plasmid#11648PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInserthPrion, GFP, tTR-KRAB, Tet-on
UseLentiviralExpressionMammalianAvailable SinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCfB2188
Plasmid#67296PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.DepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
PML-3/pSG5
Plasmid#50939Purposeexpression of PML with Sumo sites destroyedDepositorInsertPML-3 (PML Human)
TagsHAExpressionMammalianMutationK65R, K160R, K490R (Sumo modification sites)PromoterSV40Available SinceJan. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-inLuc
Plasmid#58955PurposeHIV-1 transfer vector, encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is silent in the transfected cells and expressed in the infected cells.DepositorInsertinLuc
UseLentiviralPromoterCMVAvailable SinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSL1-E2C
Plasmid#180421PurposeKan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes an E2 Crimson promoter.DepositorInsertE2 Crimson
ExpressionBacterialAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVPT-rtTR-KRAB
Plasmid#11777PurposeTet-regulated (Tet-off) lentiviral vector for transgene (mPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertmPGK, GFP, rtTR-KRAB, Tet-off
UseLentiviralExpressionMammalianAvailable SinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCSIIhyg-RGECO1.0
Plasmid#175813PurposeThe lentiviral vector for R-GECO1.0, a genetically encoded red fluorescent Ca2+ sensor.DepositorInsertRGECO1.0
UseLentiviralExpressionMammalianAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
p414GPD_Hsp82viiB
Plasmid#61709PurposeYeast plasmid with Hsp82 heterodimer construct.DepositorInsertHsp82viiB (HSP82 Budding Yeast)
Tags6xHisExpressionYeastMutationHeteromeric coiled coil inserted after amino acid…PromoterGPD1Available SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I
Plasmid#208183Purposemistranslation GFP and mScarlet-I positive controlDepositorInsertsGFPmut2
mScarlet-I
UseReporterExpressionBacterialPromoterPtet+dnaK P1Available SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Marathon-RT for Split-PE (pEH59)
Plasmid#190108PurposeExpresses Marathon-RT (human codon optimization)DepositorInsertMarathon-RT
TagsbpNLSExpressionMammalianPromoterCMVAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B4
Plasmid#31021DepositorInsertLacZ + BsaI restriction sites
Available SinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-4493NES(Booster-PKA)
Plasmid#184709PurposePKA biosensorDepositorInsertBooster-PKA
ExpressionMammalianAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNO183
Plasmid#101068PurposeppsR2-bphP1 bicistronic operon plasmidDepositorInsertsUseSynthetic BiologyExpressionBacterialMutationinsert guanine at position 430 to fix frameshift …Available SinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Gi-c010
Plasmid#173080PurposeExpression of recombinant fusion protein in E. coliDepositorInsertGAP
TagsmCitrineExpressionBacterialMutationmCitrine + last 10 residues of GNA1, codon optimi…Available SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
NbAlfa-LgBit
Plasmid#201483PurposeNanoBit detection of ALFA-tagged proteinDepositorInsertNbALFA-LgBit
TagsLgBitExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTEF6-7
Plasmid#124583PurposeReporter plasmid with constitutively expressed mCherry and YFP (eCitrine)DepositorInsertmCherry
ExpressionYeastAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6 MCV sTco
Plasmid#40201DepositorInsertMCPyV sT codon optimized
ExpressionMammalianPromoterCMVAvailable SinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCfB2189
Plasmid#67532PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2191
Plasmid#67538PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDD363
Plasmid#91829PurposeLoxP-flanked SEC donor for the SapTrap cloning systemDepositorInsertLoxP-SEC-LoxP
UseCRISPR and Cre/LoxExpressionWormAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD379
Plasmid#91834PurposeSapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffoldDepositorTypeEmpty backboneUseCRISPRExpressionWormAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherryGGBsMTS
Plasmid#133625PurposeExpression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.DepositorInsertmCherryGGBsMTS
ExpressionBacterialAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GluA1-scFv-mNeon-KDEL
Plasmid#230056PurposeAAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 tagged with mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.DepositorInsertAnti-GluA1 single chain variable fragment (scFv)
UseAAVTagsER retrieval motif (KDEL) and mNeonExpressionMammalianPromoterhuman synapsinAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfB2225
Plasmid#67553PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B6
Plasmid#31023DepositorInsertLacZ + BsaI restriction sites
Available SinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B5
Plasmid#31022DepositorInsertLacZ + BsaI restriction sites
Available SinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLX302 LacZ-V5 puro
Plasmid#98579PurposeLentiviral vector for constitutive expression of human LacZ with C-terminal V5 tagDepositorInsertLacZ
UseLentiviralTags2x V5 tagExpressionMammalianAvailable SinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSEED-OLLAS:HA
Plasmid#219647PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest methodDepositorInsertOLLAS:HA SEED Cassette
Available SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 TNRC6C
Plasmid#20023DepositorInsertTrinucleotide repeat containing 6C (TNRC6C Human)
UseEntry vectorAvailable SinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B3
Plasmid#31020DepositorInsertLacZ + BsaI restriction sites
Available SinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B1
Plasmid#31018DepositorInsertLacZ + BsaI restriction sites
Available SinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDD121
Plasmid#91833PurposeCas9 expression in C. elegansDepositorInsertCas9
UseCRISPRExpressionWormMutationCodon optimized and with synthetic introns for C.…Available SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-PKG-hNo.7
Plasmid#175812PurposeThe lentiviral vector for PKG #7, a genetically encoded FRET biosensor for monitoring cGMP concentration.DepositorInsertPKG #7
UseLentiviralExpressionMammalianAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropVn
Plasmid#184873PurposeChromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.DepositorInsertlacZalpha,ccdB
ExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits