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We narrowed to 12,288 results for: BASE

Showing: 11401 - 11450 of 12288 results

  1. pKK-RNAi-nucEGFPmiR-FLAG-TEV

    Plasmid
    #105807
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal FLAG tag.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    FLAG-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105807-plasmid-map-sequence-id-203410

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  8. pKK-RNAi-nucCHERRYmiR-TEV-FLAG

    Plasmid
    #105813
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal FLAG.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    TEV-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    105813-plasmid-map-sequence-id-204858

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  10. LSB-hsa-miR-92a-1-5p

    Plasmid
    #103750
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-92a-1-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-92a-1-5p target (MIR92A1 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103750-plasmid-map-sequence-id-196548

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  13. pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

    Plasmid
    #85821
    Purpose
    iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    Zf Ubi::cMyc oDam_f_GFPoNLS
    Use
    Tol2 based vector
    Tags
    cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…
    Expression
    Mutation
    L122A. Codon optimization compatible to work in M…
    Promoter
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85821-plasmid-map-sequence-id-170479

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  16. pKK-RNAi-nucCHERRYmiR-TEV-EGFP

    Plasmid
    #105814
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal EGFP.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    TEV-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105814-plasmid-map-sequence-id-203426

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  20. pcDNA3-InPTapp-Kras

    Plasmid
    #235999
    Purpose
    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4)P2. Targeted to plasma membrane.
    Depositor
    Jin Zhang
    Article
    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1
    Insert
    InPTapp-Kras (PLEKHA1 Human, Synthetic)
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    235999-plasmid-map-sequence-id-473745

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  21. pcDNA3-InPGrp-Kras

    Plasmid
    #236001
    Purpose
    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to plasma membrane.
    Depositor
    Jin Zhang
    Article
    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1
    Insert
    InPGrp-Kras (Cyth3 Rat, Synthetic)
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    236001-plasmid-map-sequence-id-473749

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  22. pcDNA3-Endo-InPGrp

    Plasmid
    #236003
    Purpose
    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to endosomes.
    Depositor
    Jin Zhang
    Article
    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1
    Insert
    Endo-InPGrp (Cyth3 Rat, Synthetic)
    Use
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger), 6xHIS -…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    236003-plasmid-map-sequence-id-473735

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  24. PB-TRE3G-dN40-TP53-CCRB

    Plasmid
    #236774
    Purpose
    A piggybac-based cloning vector containing TRE3G promoter-driven short-form of TP53 and CAG promoter-driven nuclear-localized Clover-P2A-rtTA-IRES-BSD.
    Depositor
    Kazutoshi Takahashi
    Article
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Insert
    TP53 (TP53 Human)
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-40
    Promoter
    TRE3G promoter and TRE3G promoter, CAG promoter
    Available since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    236774-plasmid-map-sequence-id-473566

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  25. PB-TRE3G-TP53-CCRB

    Plasmid
    #236773
    Purpose
    A piggybac-based cloning vector containing TRE3G promoter-driven full-length TP53 and CAG promoter-driven nuclear-localized Clover-P2A-rtTA-IRES-BSD.
    Depositor
    Kazutoshi Takahashi
    Article
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Insert
    TP53 (TP53 Human)
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G promoter and TRE3G promoter, CAG promoter
    Available since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    236773-plasmid-map-sequence-id-473536

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  26. PB-U6-CNCB_sgEIF3D-4

    Plasmid
    #236766
    Purpose
    A piggybac-based vector containing mouse U6 promoter-driven EIF3D sgRNA #4 and CAG promoter-driven nuclear-localized Clover-P2A-BSR.
    Depositor
    Kazutoshi Takahashi
    Article
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Insert
    EIF3D sgRNA-4 (EIF3D Human)
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse U6 promoter and mouse U6 promoter, CAG prom…
    Available since
    May 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    236766-plasmid-map-sequence-id-473592

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  29. pGECPL.BC10.MG

    Plasmid
    #223822
    Purpose
    Contains Cas9-editable barcode, marking guide (MG) for lineage tracing, Cre for Cas9 activation and loxP-mediated gene deletion, and Firefly Luciferase (FLuc) for live luminescence-based monitoring.
    Depositor
    Dawid Nowak
    Article
    Serio et al Cancer Discov. 2024 Jul 5. doi: 10.1158/
    Insert
    Ef1a-Driven Cre-P2A-FLuc
    Use
    CRISPR, Cre/Lox, Lentiviral, Luciferase, and Mous…
    Tags
    P2A
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    223822-plasmid-map-sequence-id-443651

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  31. pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro

    Plasmid
    #211821
    Purpose
    PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment
    Depositor
    Erik Sontheimer
    Article
    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.
    Insert
    SpCas9-ABE8e, tracrRNA and Puromycin resistance gene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    chicken β-actin promoter, U6 promoter and hPGK pr…
    Available since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    211821-plasmid-map-sequence-id-418440

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  34. pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)

    Plasmid
    #208977
    Purpose
    Entry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208977-plasmid-map-sequence-id-416230

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  39. pCR2.1_HBB_A

    Plasmid
    #87848
    Purpose
    pCR2.1-based plasmid holding a 328-bp fragment containing the exon-1-exon-2 splice border of aberrant HBB[IVSI-110(G>A)] human β-globin mRNA
    Depositor
    Marina Kleanthous
    Article
    Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:
    Insert
    Homo sapiens hemoglobin subunit beta (HBB), Aberrant cDNA fragment (Exon1/19nt mispliced Intron1 + Exon2) (HBB Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    HBB(IVSI-110) β-thalassaemia misplicing mutation …
    Promoter
    Available since
    Oct. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    87848-plasmid-map-sequence-id-313713

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  40. pNB1-LOX

    Plasmid
    #154058
    Purpose
    Level 1, Position 3 Golden Gate vector. ZmUbi-5'UTR:loxP-GUS-nosT-loxP-NAM-B1-nosT
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Insert
    LoxP-flanked GUS and TtNAM-B1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    The TtNAM-B1 gene sequence was domesticated to re…
    Promoter
    ZmUbi
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154058-plasmid-map-sequence-id-313109

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  41. pRcCMV-STAT31F

    Plasmid
    #134725
    Purpose
    Expresses chimeric STAT3-STAT1 protein with human STAT3 amino acids 1-514 joined to human STAT1 at amino acid 509, with a FLAG epitope tag at the C-terminus. Based on addgene plasmid #8691 and #8707
    Depositor
    Jim Darnell, Curt Horvath
    Article
    Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.
    Insert
    STAT3-STAT1 Fusion (STAT3, STAT1 Human)
    Use
    Tags
    FLAG and human STAT3 aa 1-514 joined to human STA…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    134725-plasmid-map-sequence-id-272781

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  42. pRcCMV-STAT13F

    Plasmid
    #134723
    Purpose
    Expresses chimeric STAT1-STAT3 protein with human STAT1 amino acids 1-508 joined to human STAT3 at amino acid 515, with a FLAG epitope tag at the C-terminus. Based on addgene plasmids #8691 and #8707
    Depositor
    Jim Darnell, Curt Horvath
    Article
    Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.
    Insert
    STAT1-STAT3 fusion (STAT3, STAT1 Human)
    Use
    Tags
    FLAG and human STAT1 aa1-508 joined to human STAT…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    134723-plasmid-map-sequence-id-272780

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Showing: 11401 - 11450 of 12288 results