We narrowed to 22,939 results for: REP

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pAS_4xG1 PylT FLAG-G1 PylRS Y125A

Plasmid

#154769

Purpose

plasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)

Tags

FLAG

Expression

Mammalian

Mutation

Y125A

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Ef1a-hMGT-PGK-puro

Plasmid

#172394

Purpose

Lentiviral expression vector for human direct cardiac reprogramming

Depositor

Li Qian

Article

Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

Insert

Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)

Use

Lentiviral

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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2XMyc-LRRK2-COR

Plasmid

#25069

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Contains only the COR domain aa1408-1850

Available Since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-Mito-ExRai-AMPKAR

Plasmid

#192448

Purpose

ExRai-AMPKAR targeted to the mitochondrial outer membrane.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

Mito-ExRai-AMPKAR

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-3xAU1

Plasmid

#129572

Purpose

Empty backbone for AU1 tag

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Type

Empty backbone

Tags

3xAU1 and GST

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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His6_MBP_FXR2P_P474S_P493N

Plasmid

#198695

Purpose

Expression of human FXR2 protein in E. coli

Depositor

Simpson Joseph

Article

Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

Insert

FXR2P (FXR2 Human)

Tags

MBP

Expression

Bacterial

Mutation

Changed proline 474 to serine and proline 493 asp…

Promoter

T7

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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aL-mEos3.2

Plasmid

#113154

Purpose

constrained LFA-1-mEos3.2 fusion

Depositor

Timothy Springer

Article

Moore et al Cell Rep. 2018 Feb 13;22(7):1903-1912. d

Insert

full-length human aL with mEOS3.2 in betapropeller (ITGAL Human)

Tags

mEOS3.2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 Srap1

Plasmid

#89691

Purpose

Expresses murine Srap1 in bacteria

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

Murine SRAP domain ortholog Srap1 (Hmces Mouse)

Tags

GST

Expression

Bacterial

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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PE7_AAVS1_knockin_HDR_donor

Plasmid

#223001

Purpose

HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Hdr donor

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF1α

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRc/CMV HA TrkA

Plasmid

#39977

Depositor

Yves-Alain Barde

Article

Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

Insert

TrkA (Ntrk1 Rat)

Tags

BM40 signal sequence and HA

Expression

Mammalian

Mutation

endogenous signal sequence was replaced with BM40…

Promoter

CMV

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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pMK462 (Hygro-P2A-mAB)

Plasmid

#214388

Purpose

mAID-BromoTag for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

Hygro-P2A-mAID-BromoTag

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-AAV2cap

Plasmid

#198014

Purpose

For high-level expression of the AAV2 cap gene, suitable for use with pCMV-Rep78/68 SSM library

Depositor

George Church

Article

Jain et al Elife. 2024 Mar 27;12:RP87730. doi: 10.7

Insert

AAV2 cap

Use

AAV

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pVC-FUS-R521C

Plasmid

#175067

Purpose

expresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cells

Depositor

Arnold Boersma

Article

Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

Insert

FUS glycine rich protein (FUS Human)

Tags

HA and mVenus + mCherry

Expression

Mammalian

Mutation

changed Arginin 521 to Cystein; mVenus: C-Termina…

Promoter

CMV Promoter

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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Brp-T2A-QF2w-Hsp70-3XP3-dsRed

Plasmid

#141094

Purpose

Template plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.

Depositor

Carolyn McBride

Article

Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

Insert

bruchpilot

Use

CRISPR

Expression

Insect

Mutation

coding sequence downstream of CRISPR cut site, sy…

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HER2-DMVF-181-N

Plasmid

#87622

Purpose

N-terminal Dronpa fragment fused to the C-terminus of full-length HER2; for BiFC.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

HER2-DMVF181-N (ERBB2 Human, Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR221-YAP1 CA

Plasmid

#79502

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 S127A mutant CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

YAP1 (YAP1 Human)

Use

Gateway Cloning

Tags

3XFLAG

Mutation

S127A

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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p-mCherry-N1-iChloC

Plasmid

#85467

Purpose

improved slowChloC artificial anion conducting ChR. Codon optimized for mammalian expression (human/mouse)

Depositor

Peter Hegemann

Article

Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

Insert

iChloC

Tags

mCherry

Expression

Mammalian

Mutation

E83Q, E90R, E101S, T159C, D156N

Promoter

CMV (+enhancer)

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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TOPO pTRE

Plasmid

#68457

Purpose

TOPO construct expressing pTRE for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Tetracycline Response Element Promoter

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGK:TRL-EFS:iRFP670

Plasmid

#68475

Purpose

Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

tTR-KRAB-2A-rtTA3-2A-Luciferase
iRFP670

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

EFS and pGK

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pTRE:CloverCP-EFS:ZeoR

Plasmid

#68478

Purpose

Lentivirus for establishment of doxycycline-inducible cell line expression CloverCP

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

CloverCP
Zeocin Resistance

Use

Lentiviral

Tags

"CP" degradation domain

Expression

Mammalian

Promoter

EFS and pTRE

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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