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pAAV-EF1a-mCherry-IRES-Flpo

Plasmid

#55634

Purpose

Expresses Flpo in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV1

Inserts

mCherry
Flpo

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a and Ef1a/IRES

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Soma-jGCaMP8m

Plasmid

#169257

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

Soma-jGCaMP8m

Use

AAV

Tags

6xHis-tag

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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ATF4 6: mCD36.GFP

Plasmid

#21853

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

mCD36 (Cd36 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Only the first 13 AA of CD36 are fused to GFP

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Pmel-1_TCRB

Plasmid

#175288

Purpose

Expresses Pmel-1 TCR beta chain

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

T cell receptor beta chain (Tcrb Mouse)

Use

Suitable for ivt

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Pmel-1_TCRA

Plasmid

#175287

Purpose

Expresses Pmel-1 TCRA alpha chain

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

T cell receptor alpha chain (Tcra Mouse)

Use

Suitable for ivt

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Let-7b-GFP

Plasmid

#25404

Depositor

Sean Morrison

Article

Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

Insert

Mirlet7b (Mirlet7b Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
LV-Let7d-5p

Plasmid

#117055

Purpose

Overexpression of Let-7d-5p using miR-451 backbone to enable DICER independent expression along with orange fluorescent protein (OFP) to track miRNA expression

Depositor

Michele De Palma

Article

Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

Insert

Let-7d-5p - OFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEXfrt-SaCas9-U6-sgRosa26

Plasmid

#159915

Purpose

Mutagenesis of Rosa26

Depositor

Larry Zweifel

Insert

Rosa26 (Gt(ROSA)26Sor Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pB-mTSPAN4-mCherry

Plasmid

#210330

Purpose

Overexpression of mTSPAN4

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

mTSPAN4 (Tspan4 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET-45b-mCherry-Cbx5

Plasmid

#194547

Purpose

Bacterial Expression of 6xHis mCherry-HP1a Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

Cbx9 (HP1a)

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Puro.let-7g.Cre

Plasmid

#17409

Purpose

Depositor

Tyler Jacks

Article

Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

Insert

Let-7g

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2008

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT-DEST EVC2-Flag

Plasmid

#41005

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
pMiniT:SEP-GluN2A TKIT donor

Plasmid

#169429

Purpose

Donor DNA for TKIT knockin of SEP to NR2A in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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Puro.let-7gsm.Cre

Plasmid

#17410

Depositor

Tyler Jacks

Article

Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

Insert

Let-7g mutant

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

mutated version of let-7g

Promoter

Available since

March 11, 2008

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-GluN2B TKIT donor

Plasmid

#169431

Purpose

Donor DNA for TKIT knockin of SEP to NR2B in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGria1

Plasmid

#124853

Purpose

Mutagenesis of Gria1

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gria1 (Gria1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

Plasmid

#84561

Purpose

pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops

Depositor

Robert Singer

Article

Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

Insert

FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops

Use

Lentiviral

Tags

Flag, Suntag, oxBFP, AID (auxin-inducible degron)

Expression

Mutation

Promoter

UbC

Available since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-Sun1GFP-WPRE-pA

Plasmid

#160141

Purpose

Cre- dependent expression of Sun1GFP for nuclei isolation

Depositor

Andreas Pfenning

Article

Lawler et al J Neurosci. 2020 Nov 10. pii: JNEUROSCI.

Insert

Sun1-GFP (Sun1 Mouse)

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

N-truncated: removed amino acids 1-207, retained …

Promoter

Ef1a

Available since

March 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Bcl2

Plasmid

#195551

Purpose

Expresses Bcl2 in mammalian cells

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Bcl2 (Bcl2 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

Promoter

CMV enhancer and promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXL010-Wnt dual (GFP-Fire) reporter

Plasmid

#40588

Depositor

Sean Palecek

Article

Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

Insert

Wnt reporter sequences

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Wnt promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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