We narrowed to 2,701 results for: LRE

Showing: 881 - 920 of 2701 results

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pEGFP_mEGFP-GluA1c

Plasmid

#114136

Purpose

Mammalian expression of the cytosolic C terminus of GluA1 N-terminally tagged by mEGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

mEGFP tagged GluA1 C-terminus

Expression

Mammalian

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Limk1

Plasmid

#163414

Purpose

Expresses LIMK1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Limk1 (Limk1 Mouse)

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-oG-WPRE-SV40pA

Plasmid

#74291

Purpose

pAAV vector to express oG (optimized Glycoprotein) in a Flp-dependent manner

Depositor

Edward Callaway

Article

Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

Insert

oG (optimized Glycoprotein)

Use

AAV

Expression

Mammalian

Mutation

chimeric glycoprotein

Promoter

CAG

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgSlc17a8

Plasmid

#124848

Purpose

Mutagenesis of Slc17a8

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a8 (Slc17a8 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70

Plasmid

#62897

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_FluA/H7N9

Plasmid

#229860

Purpose

pFastBac Dual vector for co-expression of FluPolA/H7N9 polymerase heterotrimer (PA/PB1/PB2)

Depositor

Stephen Cusack

Article

Kouba et al Cell Rep. 2023 Jan 31;42(1):111901. doi:

Inserts

PA_H7N9-FluA
PB1_H7N9-FluA
PB2_H7N9-FluA

Tags

2x-Streptag and 8xHis-tag

Expression

Insect

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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mActA-FKBP-mCherry

Plasmid

#210295

Purpose

ActuAtor for FKBP-FRB CID

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

mActA-FKBP-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-p65AD-Hsp70

Plasmid

#62914

Purpose

Creating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-p65AD-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti-eCas9-sgNT

Plasmid

#140238

Purpose

Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

non-targeting sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pFL-EGFP-3xFlag-DEST

Plasmid

#134903

Purpose

Baculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tag

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Type

Empty backbone

Tags

3x-Flag

Expression

Insect

Promoter

polyhedron

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-oG-WPRE-SV40pA

Plasmid

#74292

Purpose

pAAV vector to express oG (optimized Glycoprotein) in a Cre-dependent manner

Depositor

Edward Callaway

Article

Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

Insert

oG (optimized Glycoprotein)

Use

AAV

Expression

Mammalian

Mutation

chimeric glycoprotein

Promoter

CAG

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-TSen

Plasmid

#209916

Purpose

A FRET biosensor for ADAM17 activity

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TSen

Tags

ECFP and YPet

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-dVP16AD-Hsp70

Plasmid

#62905

Purpose

Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-dVP16AD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-PD-L1-SNAP

Plasmid

#223577

Purpose

Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-L1 (CD274 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-dVP16AD-Hsp70

Plasmid

#62908

Purpose

Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-dVP16AD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAGM65881

Plasmid

#153215

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-shWT1_B

Plasmid

#217637

Purpose

Dox inducible shRNA targeting WT-1

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

shWT1_B (WT1 Human)

Use

RNAi

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pACYC-yhaM-RFP

Plasmid

#207651

Purpose

Overexpression of native L-cysteine desulfidase and RFP in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

yhaM

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMK281 (mCherry2-Hygro)

Plasmid

#72797

Purpose

C-terminal tagging with mCherry2 using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mCherry2-Hygro

Use

CRISPR

Tags

mCherry2

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-Gal4DBD-Hsp70

Plasmid

#62904

Purpose

Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4DBD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCF287_EFS-TiCas9-P2A-Puro

Plasmid

#211646

Purpose

EFS-TiCas9-P2A-Puro. Tamoxifen-inducible SpyCas9.

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

Tamoxifen-inducible SpyCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-LexA::QFAD-Hsp70

Plasmid

#62949

Purpose

Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-LexA::QFAD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGGS_VP30_MARV

Plasmid

#135472

Purpose

Mammalian expression vector encoding Marburg virus VP30 gene under control of the AG promoter

Depositor

Elke Mühlberger

Article

Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

Insert

VP30

Expression

Mammalian

Mutation

None

Promoter

AG

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-QF2-Hsp70

Plasmid

#62945

Purpose

Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-QF2-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-3X Gal80-Hsp70

Plasmid

#62951

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 0. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pET151-GyrA

Plasmid

#216229

Purpose

S. aureus USA300 GyrA Codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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R26-EGFP HMEJ donor vector

Plasmid

#137927

Purpose

ROSA26 knock-in vector with homology-mediated end joining(HMEJ)-based method

Depositor

Hiroshi Kiyonari

Article

Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

Insert

SA-EGFP-bpA with homology arms

Use

Mouse Targeting

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMy-BirA-T2A-eGFP

Plasmid

#50565

Purpose

Retroviral vector encoding for GFP and the biotinylating enzyme BirA

Depositor

Rafael Casellas

Article

Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

Insert

Ampicilin

Use

Retroviral

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-H2B-Venus

Plasmid

#87614

Purpose

Venus (YFP) fused to histone H2B.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

H2B-Venus (H2BC8 Human, Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-DIO-oG-WPRE-hGH

Plasmid

#74290

Purpose

pAAV vector to express oG (optimized Glycoprotein) in a Cre-dependent manner

Depositor

Edward Callaway

Article

Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

Insert

oG (optimized Glycoprotein)

Use

AAV

Expression

Mammalian

Mutation

chimeric glycoprotein

Promoter

Ef1a

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Bmp4

Plasmid

#163588

Purpose

Expresses BMP-4 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp4 (Bmp4 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pIVT/Smarcc1

Plasmid

#122145

Purpose

For making Smarcc1 RNA

Depositor

Carmen Williams

Article

Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

Insert

Smarcc1 (Smarcc1 Mouse)

Use

For in vitro transcription

Promoter

T7

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-Cdk9 T186A

Plasmid

#28101

Depositor

Andrew Rice

Article

Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

Insert

Cdk9 (CDK9 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Contains a T186A mutation in Cdk9

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
ER-ABKAR

Plasmid

#61508

Purpose

Expression of AMPK biosensor targeted to the endoplasmic reticulum

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

ER targeting sequence from CYP450 2C1 (see commen…

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pCX-NTOM-SpiCee

Plasmid

#140902

Purpose

mammalian expression of NTOM-SpiCee, a mitochondria-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

NTOM-SpiCee

Tags

mRFP

Expression

Mammalian

Promoter

CAG

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-LifeAct-FRB-Venus-173-N

Plasmid

#87616

Purpose

FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-FRB-Venus173-N

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-FLEX-SaCas9-U6-sgSlc18a2

Plasmid

#124849

Purpose

Mutagenesis of Slc18a2

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc18a2 (Slc18a2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
3xFLAG-Halo-Dyskerin

Plasmid

#207578

Purpose

CRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locus

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

HaloTag DKC1

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-Mu-switch-R-loop

Plasmid

#134899

Purpose

Generation and purification of R-loops for in vitro biochemistry

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

mouse IgH switch repeat region

Use

Synthetic Biology

Promoter

T7

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-KS-attB2-SA(0)-T2A-LexA::QFAD-Hsp70

Plasmid

#62947

Purpose

Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-LexA::QFAD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 2,701 results for: LRE