We narrowed to 26,180 results for: STI

1. pcDNA3.0 FLAG TRPV4

   Plasmid

   #45751

   Depositor

   Robert Lefkowitz

   Article

   Shukla et al J Biol Chem. 2010 Sep 24;285(39):30115-2

   Insert

   TRPV4 (Trpv4 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Piggybac-TetO-hNurr1-blast

   Plasmid

   #176485

   Purpose

   TetO-hNurr1 in a PiggyBac vector with Blasticidin resistance

   Depositor

   Marius Wernig

   Article

   Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

   Insert

   Nurr1 (NR4A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hprtSAGFP

   Plasmid

   #41594

   Purpose

   In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences

   Depositor

   Maria Jasin

   Article

   Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.

   Insert

   SA-GFP reporter
   

   Use

   Mouse Targeting; Dsb repair reporter substrate

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

   Plasmid

   #185913

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRosetta_v2

   Plasmid

   #136477

   Purpose

   Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   PGK

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV_TRET_hNgn1_UBC_Bla

   Plasmid

   #61473

   Purpose

   3rd generation lentiviral vector; TetON promoter driving human Ngn1; constitutive expression of Blasticidine selection marker

   Depositor

   Ron Weiss

   Article

   Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

   Inserts

   Ngn1 (NEUROG1 Human)
   Bla
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-Cys-ELP(40nm)-LPETGG

   Plasmid

   #90475

   Purpose

   Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   Cys-ELP(40nm)-LPETGG
   

   Use

   Tags

   Cysteine and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. iTol2Amp-γ-crystallin:RFP

   Plasmid

   #108455

   Purpose

   This plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.

   Depositor

   Nadia Mercader Huber

   Article

   Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

   Insert

   Tol2-Amp-y-cryst-mCherry
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-GGG-ELP(40nm)-Cys

   Plasmid

   #91571

   Purpose

   Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   GGG-ELP(40nm)-Cys
   

   Use

   Tags

   Cysteine and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3 Barr2 flag

    Plasmid

    #14685

    Depositor

    Robert Lefkowitz

    Article

    Laporte et al Proc Natl Acad Sci U S A. 1999 Mar 30. 9

    Insert

    beta-arrestin 2 (Arrb2 Rat)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pLenti_Blast_DEST mouse_p65promoter_mouse_p65-mRuby2

    Plasmid

    #124903

    Purpose

    Lentiviral vector to express p65-mRuby2 under p65 promoter (With blasticidin resistance)

    Depositor

    Markus Covert

    Article

    Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

    Insert

    p65 promoter- p65 CDS-mRuby2
    

    Use

    Lentiviral

    Tags

    mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CMV-mEGFP-PKCa-C1

    Plasmid

    #112264

    Purpose

    ITRACKalpha Donor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    protein kinase C alpha (PRKCA Bovine)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    A11V (please see depositor comments below)

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCLucIPZ

    Plasmid

    #53222

    Purpose

    Constitutive expression of Cypridina luciferase, puromycin N-acetyl-transferase and shRNAmir of interest

    Depositor

    Thorsten Stiewe

    Article

    Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

    Inserts

    Cypridina luciferase
    non-silencing shRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pET15b SARS-CoV PLpro

    Plasmid

    #61693

    Purpose

    inducible expression of SARS PLpro protease

    Depositor

    Michael Johnson

    Article

    Lee et al ChemMedChem. 2013 Aug;8(8):1361-72. doi:

    Insert

    SARS-CoV PLpro
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pZS*11_4enh

    Plasmid

    #149423

    Purpose

    Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).

    Depositor

    Stan Fields

    Article

    Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

    Inserts

    rbcS-E9 enhancer
    35S core enhancer
    Cab-1 enhancer
    AB80 enhancer
    

    Use

    Tags

    T120>A

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAGH42

    Plasmid

    #107249

    Purpose

    Vipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).

    Depositor

    Erin O'Shea

    Article

    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

    Insert

    vipp1-gfp
    

    Use

    Suicide vector for destined for double homologous…

    Tags

    mGFPmut3

    Expression

    Bacterial

    Mutation

    Promoter

    native promoter of sll0617

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TL1 alphaL_DdCBE 1-2-3 (right, DddA11-N)

    Plasmid

    #221997

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE repeats (alphaL-1/2/3 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: S1330I, A1341V, N1342S, E1370K, T1380I

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHDR-CDKN2A-Ex2-CMV-EGFP

    Plasmid

    #110734

    Purpose

    Recombination template for replacing CDKN2A Exon2 with CMV-EGFP

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    EGFP
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CMV-PKCa-mEGFP-N1

    Plasmid

    #112269

    Purpose

    IDOCKSalpha Donor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    Protein Kinase C alpha (PRKCA Bovine)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    A11V (please see depositor comments below)

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-GGG-ELP(120nm)-Cys

    Plasmid

    #91572

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    GGG-ELP(120nm)-Cys
    

    Use

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGLucIPZ

    Plasmid

    #228703

    Purpose

    Constitutive expression of Gaussia luciferase, puromycin N-acetyl-transferase and shRNAmir of interest

    Depositor

    Thorsten Stiewe

    Article

    Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

    Inserts

    Gaussia luciferase
    non-silencing shRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. Cdk6-DN-HA

    Plasmid

    #1869

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk6 (CDK6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Dominant negative, D to N in conserved KLAD*FGLAR…

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

23. pET28a-Cys-ELP(120nm)-LPETGG

    Plasmid

    #90472

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    Cys-ELP(120nm)-LPETGG
    

    Use

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSunS

    Plasmid

    #171672

    Purpose

    SunS expression in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

    Insert

    Glycosyltransferase SunS (sunS Bacillus subtilis (strain 168))
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CMV-PKCg-mEGFP-N1

    Plasmid

    #112270

    Purpose

    IDOCKSgamma Donor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    protein kinase c gamma (Prkcg Mouse)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGWB21_TaNAM-A1

    Plasmid

    #124572

    Purpose

    Expresses full-length TaNAM-A1 with an N-terminal 10x Myc tag under the 35s promoter.

    Depositor

    Cristobal Uauy

    Article

    Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

    Insert

    TaNAM-A1
    

    Use

    Tags

    10xMyc

    Expression

    Plant

    Mutation

    Promoter

    35s

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Cdk6-DN

    Plasmid

    #1867

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk6 (CDK6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Dominant negative, D to N in conserved KLAD*FGLAR…

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

28. pCDNA3.1 AT1AR-324delta

    Plasmid

    #45635

    Depositor

    Robert Lefkowitz

    Article

    Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

    Insert

    AT1AR-324delta (Agtr1a Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    added stop codon at 324 aa

    Promoter

    CMV

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pET28a-T5-ASG_ClpXP

    Plasmid

    #153299

    Purpose

    Expresses acoustic sensor genes or ClpXP-sensitive gas vesicles in E. coli Nissle 1917

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    ASG
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMM1162

    Plasmid

    #112535

    Purpose

    pZE2-hrtR-RBS3-chuA - LuxCDABE transcriptional unit deletion of plasmid pMM643, ColE1 origin, KanR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Inserts

    HrtR
    ChuA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23107 and ProD

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMM549

    Plasmid

    #112531

    Purpose

    pZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Inserts

    HrtR
    ChuA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23107 and ProD

    Available since

    Feb. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET50del_SynPspA_R44K/E126Q/E179Q

    Plasmid

    #223819

    Purpose

    Bacterial expression of SynPspA R44K/E126Q/E179Q with N-terminal 6xHis-Tag and 3C cleavage site

    Depositor

    Carsten Sachse

    Article

    Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

    Insert

    SynPspA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R44K, E126Q, E179Q

    Promoter

    Available since

    Aug. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBtA3

    Plasmid

    #238985

    Purpose

    Mammalian expression of bovine arrestin-3 short isoform

    Depositor

    Vsevolod Gurevich

    Article

    Hanson et al J Mol Biol. 2007 Apr 27;368(2):375-87. d

    Insert

    arrestin-3 short (ARR3 Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. TL1 alphaDdCBE 3_NC (right, DddA11-C)

    Plasmid

    #222005

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_NC right)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: T1413I

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. TL1 alphaDdCBE A1 (DddA6-C) - TL1 alphaL_DdCBE 2 (left, DddA11-C)

    Plasmid

    #221994

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: T1413I

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TL1 alphaDdCBE T2 (DddA6-N)

    Plasmid

    #221995

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TL1 alphaL_DdCBE 1 (left, DddA11-C)

    Plasmid

    #221996

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alphaL-1 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: T1413I

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TL1 alphaL_DdCBE 4 (right, DddA11-N)

    Plasmid

    #221999

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE repeats (alphaL-4 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: S1330I, A1341V, N1342S, E1370K, T1380I

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TL1 alphaDdCBE 1-2 (right, DddA11-N)

    Plasmid

    #222000

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-1/2 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | TALE: S1330I, A1341V,…

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TL1 alphaDdCBE 2 (left, DddA11-C)

    Plasmid

    #222001

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-2 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: T1413I

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. TL1 alphaDdCBE 3_CN (left, DddA11-C)

    Plasmid

    #222002

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_CN left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: T1413I

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pYPQ121-Split-TALED

    Plasmid

    #218171

    Purpose

    Express plastid TALE-linked deaminases (TALED), composed of TALE, split DddA originated from Burkholderia cenocepacia, and an engineered deoxyadenosine deaminase derived from the E. coli TadA protein

    Depositor

    Yiping Qi

    Article

    Wang et al Plant Biotechnol J. 2024 May 6. doi: 10.

    Insert

    TALE-Intron-N-DddAtox half (G1397N)-T2A-TALE-C-DddAtox half (G1397C)-TadA8e
    

    Use

    Plastid base editing

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pYPQ121-mTCBE

    Plasmid

    #218172

    Purpose

    Express plastid monomeric TALE-linked CBE, composed of TALE, a catalytically impaired, full length DddA variant (E1347A), a human APOBEC3A variant (hA3A-Y130F), and UGI

    Depositor

    Yiping Qi

    Article

    Wang et al Plant Biotechnol J. 2024 May 6. doi: 10.

    Insert

    TALE-Intron-hA3A-Y130F-DddA full (E1347A)-UGI
    

    Use

    Plastid base editing

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pWR88

    Plasmid

    #203422

    Purpose

    empty BLINCAR plasmid for payload delivery, no payload, no genomic target

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    CAR copy 1
    P(TEF1)/coKanMX/T(ACT1) + P(TDH3)/coCBG/T(ADH2)
    CAR copy 2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pVAX1-VSVG-INDIANA2

    Plasmid

    #196623

    Purpose

    Express VSV-G protein in mammalian cells.

    Depositor

    Nicolas Manel

    Article

    Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

    Insert

    VSV-G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pTF

    Plasmid

    #162784

    Purpose

    Protein expression in CHO cells - C-terminal FLAG and 10xhis tags are coded

    Depositor

    Ivan Vorobiev

    Article

    Kolesov et al Diagnostics (Basel). 2022 Feb 3;12(2). p

    Type

    Empty backbone

    Use

    Tags

    10xHis and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CHO Elongation factor 1A

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pSunS 1-335

    Plasmid

    #171673

    Purpose

    SunS 1-335 expression in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

    Insert

    Glycosyltransferase SunS (sunS Bacillus subtilis (strain 168))
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. FoxFTVC+bpFOG_MEK-S216D, S220E

    Plasmid

    #107522

    Purpose

    Constitutive activation of FGF/MAPK pathway by MEK-S216D, S220E mutant

    Depositor

    Lionel Christiaen

    Article

    Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

    Insert

    MEK
    

    Use

    Tags

    Expression

    Mutation

    changed Serine 216 to Aspartic Acid and Serine 22…

    Promoter

    TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. MTK0_004

    Plasmid

    #123961

    Purpose

    Encodes the Cas9 cROSA26 homology destination with Blasticidin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    cRosa26 destination
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCR8_TaNAM-A1

    Plasmid

    #124574

    Purpose

    TaNAM-A1 CDS in the Gateway-compatible pCR8 vector.

    Depositor

    Cristobal Uauy

    Article

    Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

    Insert

    TaNAM-A1
    

    Use

    Topo gateway cloning vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge