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Plasmid#113884PurposeMammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFPDepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepExpressionMutationPromoterpHR-CMV-TetO2Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only
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U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP
Plasmid#194283PurposeEF1a-dCas9-KRAB-GFP with Slc1a3 sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MKI-MUT-P2A-GFP-DOX-N
Plasmid#229408PurposeLentiviral or overpexression of cDNADepositorInsertMKI-MUT-P2A-GFP
UseLentiviralTagsFLAGExpressionMammalianMutationinteraction mutant L109G; L143G (Control mutant)PromoterTRE3GAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-NIR-GECO2-P2A-EGFP
Plasmid#198063PurposeThe lentiviral vector for NIR-GECO2 and EGFPDepositorInsertNIR-GECO2
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-NIR-GECO1-P2A-EGFP
Plasmid#198065PurposeThe lentiviral vector for NIR-GECO1 and EGFPDepositorInsertNIR-GECO1
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-miRFP704nano(S86C)-P2A-EGFP
Plasmid#197372PurposeThe lentiviral vector for miRFP704nano(S86C) and EGFPDepositorInsertmiRFP704nano(S86C)
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationS86C mutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-miRFP718nano(S86C)-P2A-EGFP
Plasmid#197373PurposeThe lentiviral vector for miRFP718nano(S86C) and EGFPDepositorInsertmiRFP718nano(S86C)
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationS86C mutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-iRFP713(V256C)-P2A-EGFP
Plasmid#198061PurposeThe lentiviral vector for iRFP713(V256C) and EGFPDepositorInsertiRFP713(V256C)
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationV256C mutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_GFP_AAVS1
Plasmid#141211PurposepDECKO_GFP_AAVS1_1801. Targeting, non-essential locus controlDepositorInsertgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_GFP
Plasmid#141210Purposeempty GFP vector for cloning sgRNA or pgRNAsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pfNL43-dE-EGFP
Plasmid#36865DepositorInsertfNL43-dE-EGFP
UseLentiviral and RetroviralTagsExpressionMammalianMutationinserted EGFP after 6350 nucleotide of original N…PromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X1
Plasmid#59200PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertkrt1-ex1
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X7
Plasmid#59231PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertkrt5-ex3-5
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceDec. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X8
Plasmid#59232PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertkrt5-ex7
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X5
Plasmid#59229PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertkrt5 ex1
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X4
Plasmid#59228PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertKrt4-ex7-9 and 3' UTR
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X6
Plasmid#59230PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertkrt5 ex2
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X3
Plasmid#59227PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertKrt4-ex7-9
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
plix403-V5-N-mEGFP
Plasmid#223957PurposeGateway cloning of genes of interest with an N-terminal V5-mEGFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseLentiviralTagsV5-mEGFPExpressionMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFV GLP1R(S301A)-EGFP
Plasmid#158123PurposeLentiviral plasmid for expression of GLP1R(S301)-EGFP fusion with SFFV promoter.DepositorInsertGLP1R (GLP1R )
UseLentiviralTagsEGFPExpressionMammalianMutationSerine 301 changed to alanine to enhance membrane…PromoterSFFVAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-GFP1-10-IRES-Puro
Plasmid#211467PurposeLentivirus backbone expressing GFP1-10DepositorInsertGFP1-10
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-F0ATPMLS-mCherry-GFP1-10
Plasmid#211466PurposeLentivirus backbone expressing mCherry and GFP1-10 fusion protein with a mitochondrial localization signalDepositorInsertmito-mCherry-GFP1-10
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO.puro_shGFP
Plasmid#110421PurposeshGFP control, silence GFP gene, doxycycline inducible, puromycin selectionDepositorInsertGFP
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1/TO (RNA PolIII)Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE3G-PYL1-sfGFP-PML
Plasmid#167011Purposeexpression of PYL1-SfGFP-PML, lentivirus vectorDepositorInsertPML (PML Human)
UseLentiviralTagsGFP-PMLExpressionMammalianMutationPromoterAvailable sinceJune 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (GFP)
Plasmid#80924PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR pUAS-dGFP pPGK-iRFP
Plasmid#176530PurposeLentiviral vector expressing a UAS-driven destabilized GFP, as well as a PGK promoter-driven iRFP marker.DepositorInsertsdGFP
iRFP
UseLentiviralTagsExpressionMammalianMutationPromoterPGKp and UASpAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRRL-GFPGO2(NGG)-PGK-Neo
Plasmid#136891PurposeLentivirus for base editing activatable GFP expression in mammalian cellsDepositorInsertGFPGO2(NGG)
UseLentiviralTags2xNLSExpressionMutationATG>ACGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRL-mU6-GFPGO-IRES-mScarletI
Plasmid#136895PurposeLentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO2.DepositorInsertGFPGO-IRES-mScarletI
UseLentiviralTags3xNLS on mScarletExpressionMutationATG>ACGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV CDHblast Rev(V16D)-EGFP-V5
Plasmid#175165PurposeLentiviral expression of Rev(V16D)-EGFP-V5DepositorInsertrev
UseLentiviralTagsV5ExpressionMammalianMutationV16DPromoterAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRRL-GFPGO2(NGC)-PGK-Neo
Plasmid#136893PurposeLentivirus for base editing activatable GFP expression in mammalian cellsDepositorInsertGFPGO2(NGC)
UseLentiviralTags2xNLSExpressionMutationATG>ACGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRL-GFPGO2(NGT)-PGK-Neo
Plasmid#136892PurposeLentivirus for base editing activatable GFP expression in mammalian cellsDepositorInsertGFPGO2(NGT)
UseLentiviralTags2xNLSExpressionMutationATG>ACGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBMN(CMV-copGFP-Luc2-Puro)
Plasmid#80389PurposeTo make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection markerDepositorInsertcopGFP-T2A-Luc2-F2A-Puromycin
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-EGFP-Human-UTROPHIN_1-261
Plasmid#222393PurposeExpress in mammalian cells the EGFP fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.DepositorInsertHuman Utrophin residues 1-261 and EGFP (UTRN Human, Homo Sapiens)
UseLentiviralTagsEGFP and Human UTROPHIN 1-261ExpressionMammalianMutationSequence is derived from ADDGENE Plasmid #26737-G…PromoterCMV promoter and enhancerAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL-U6-sgRNA-SFFV-Puro-P2A-EGFP
Plasmid#175037PurposeLentiviral delivery of sgRNA. Expresses PuroR and eGFP from SFFV promoter.DepositorInsertSp sgRNA scaffold
UseLentiviralTagsExpressionMutationPromoterU6Available sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRE-KRAB-dCas9-IRES-GFP
Plasmid#85556Purpose2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFPDepositorInsertKRAB-dCas9-IRES-GFP
UseCRISPR and LentiviralTags2x NLS, HA Tag, and KRAB domainExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
phage ubc nls ha pcp gfp
Plasmid#64539PurposeUsed to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.DepositorInsertPCP
UseLentiviralTagsEGFP, FactorXa site, HA, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
plko-shmfn2-mito-GFP-ER tethering
Plasmid#160509PurposeRescue mfn2 knockdown by tethering mitochondria and ER with GFPDepositorInsertmito-GFP-ER tethering
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRL-mU6-GFPGO2-IRES-mScarletI
Plasmid#136896PurposeLentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.DepositorInsertGFPGO2-IRES-mScarletI
UseLentiviralTags2xNLS on GFP, 3xNLS on mScarletExpressionMutationATG>ACGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-TAX1BP1 SKICH (1-140D)
Plasmid#175768PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 SKICH (1-140D)DepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutationSKICH (1-140D)PromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP
Plasmid#179717PurposeLentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFPDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-TAX1BP1 Q770A E774K
Plasmid#175770PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 Q770A E774KDepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutationQ770A E774KPromoterAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-TAX1BP1 632-639D
Plasmid#175771PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 632-639DDepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutation632-639DPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMB204: UbC-GFP-Tg_SAP30L-WPRE
Plasmid#168109PurposeLentiviral expression of Zebra Finch SAP30L fused to the C-terminal end of eGFPDepositorInsertSAP30L (SAP30L T. guttata)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterUbCAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-GFP-FLAG-cGAS
Plasmid#86675PurposeExpresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.DepositorInsertGFP-FLAG-cGAS
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57-GFP-P2A-MCS (Neo)
Plasmid#89181PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
tetO-FUW-eGFP-RHOA-Q63L
Plasmid#73081PurposeLentiviral transfer vector for tet-inducible expression of EGFP tagged human RHOA-Q63LDepositorInsertRHOA-Q63L (RHOA Human)
UseLentiviralTagseGFPExpressionMammalianMutationQ63L Constitutively activePromoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWPT-/GCCACC-mEGFP-IRES-mCherry
Plasmid#49235PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-PYL1-sfGFP-Emerin
Plasmid#121532Purposeexpression of PYL-SfGFP-Emerin, lentivirus vectorDepositorInsertEmerin (EMD Human)
UseLentiviralTagsPYL1-SFGFPExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHRSin-IRES2-EmGFP_HPC-4 Ab heavy-6xHIS
Plasmid#187358PurposeLentiviral expression of HPC-4 antibody heavy chain, and 6xHIS tagDepositorInsertHPC-4 antibody heavy chain, and 6xHIS tag
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR
Plasmid#162335PurposeLentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection markerDepositorInsertS. pyogenes sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only