We narrowed to 24,265 results for: nes

1. pLV-EF1a-KIF5AR280H-HA-IRES-Puro

   Plasmid

   #166953

   Purpose

   Lentiviral plasmid expressing HA-tagged KIF5A R280H protein with IRES-Puro from the EF1a promoter

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   KIF5A-R280H (Kif5a Mouse)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   KIF5A-R280H

   Promoter

   eF1a

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPHAGE-EF1α-Luc-C20orf24-3’UTR-VAR

   Plasmid

   #128507

   Purpose

   Lentiviral constitutive expression of Firefly luciferase under control of 3'UTR of human C20orf24 with variant from COX deficiency patient.

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-Ef1a-fDIO-JRGECO1a

   Plasmid

   #128317

   Purpose

   Expresses JRGECO1a under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

   Depositor

   Patricia Jensen

   Has Service

   AAV1

   Insert

   NES-JRGECO1a
   

   Use

   AAV

   Tags

   His Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pmNET

   Plasmid

   #35627

   Depositor

   Howard Gu

   Article

   Han et al BMC Pharmacol. 2006 Mar 3;6:6.

   Insert

   NET (Slc6a2 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLVX-Flag-CDK19-WT-IRES-ZsGreen

   Plasmid

   #68858

   Purpose

   Expresses Human CDK19 Wild-Type

   Depositor

   Matthew Shair

   Article

   Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

   Insert

   Cyclin-Dependent Kinase 19 (CDK19 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLVX-Flag-CDK19-W105M-IRES-ZsGreen

   Plasmid

   #68859

   Purpose

   Expresses Human CDK19 W105M Mutant

   Depositor

   Matthew Shair

   Article

   Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

   Insert

   Cyclin-Dependent Kinase 19 (CDK19 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   W105M

   Promoter

   EF1α

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPHAGE-CR-NENF-C-TAP

   Plasmid

   #128511

   Purpose

   Lentiviral constitutive expression of CRISPR-resistant NENF with c-terminal FLAG and HA tag.

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   NENF (NENF Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Synonymous silent mutations to F81, Y82, G83 and …

   Promoter

   CMV

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLD-puro-Cc-PARK7WT-VA

   Plasmid

   #115182

   Purpose

   Lentiviral transduction and expression of PARK7WT into any mammalian cell

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   PARK7WT (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPHAGE-C20orf24- C20orf24-3’UTR-WT

   Plasmid

   #128508

   Purpose

   Lentiviral constitutive expression of C20orf24 under control of its native WT 3'UTR of human C20orf24.

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   RAB5IF (RAB5IF Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLEX307_CR-PDHA2S293A

    Plasmid

    #115204

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S293A (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLD-puro-Cc-PARK7V51G-VA

    Plasmid

    #115183

    Purpose

    Lentiviral transduction and expression of PARK7V51G into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PARK7V51G (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Expression

    Mutation

    p.V51G

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLD-puro-Cc-PARK7C53A-VA

    Plasmid

    #115184

    Purpose

    Lentiviral transduction and expression of PARK7C53A into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PARK7C53A (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Expression

    Mutation

    p.C53A

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLD-puro-Cc-PARK7H126A-VA

    Plasmid

    #115185

    Purpose

    Lentiviral transduction and expression of PARK7H126A into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PARK7H126A (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Expression

    Mutation

    p.H126A

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLD-puro-Cc-PARK7E163K-VA

    Plasmid

    #115186

    Purpose

    Lentiviral transduction and expression of PARK7E163K into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PARK7E163K (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Expression

    Mutation

    p.E163K

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLEX307_CR-PDHA2

    Plasmid

    #115199

    Purpose

    Lentiviral transduction and expression of a CRISPR/Cas9-resistant PDHA2 into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2WT (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLEX307_CR-PDHA2S291A

    Plasmid

    #115203

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S291A (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPHAGE-C20orf24- C20orf24-3’UTR-VAR

    Plasmid

    #128509

    Purpose

    Lentiviral constitutive expression of C20orf24 under control of its native 3'UTR of human C20orf24 with variant from COX deficiency patient.

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    RAB5IF (RAB5IF Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJH124

    Plasmid

    #102951

    Purpose

    Expresses FLAG-APEX2 in Saccharomyces cerevisiae under control of GAL1 promoter

    Depositor

    Peter Espenshade

    Article

    Hwang et al Biochem J. 2016 Jun 7. pii: BCJ20160106.

    Insert

    APEX2-NES
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Dec. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPS1372

    Plasmid

    #1353

    Depositor

    Pamela Silver

    Article

    Taura et al Proc Natl Acad Sci U S A 1998 Jun 23;95(

    Insert

    pADH-NLS-NES-GFP2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pCWXPGR-pTF-ERK:MEK

    Plasmid

    #114291

    Purpose

    TET-inducible expression of ERK:MEK fusion protein

    Depositor

    Patrick Salmon

    Insert

    rERK2 and hMEK1 proteins (Mapk1 Rat, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    rat ERK2 fused to human MEK1 with a linker in bet…

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLEX_307-SOD1WT

    Plasmid

    #83444

    Purpose

    Mammalian expression of SOD1WT

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPHAGE-CR-PINK1-C-TAP

    Plasmid

    #128510

    Purpose

    Lentiviral constitutive expression of CRISPR-resistant PINK1 with c-terminal FLAG and HA tag.

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PTEN induced kinase 1 (PINK1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA tags

    Expression

    Mammalian

    Mutation

    Synonymous silent mutation in Q5 to render sgRNA …

    Promoter

    CMV

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLEX_307-SOD1E133insTT

    Plasmid

    #83446

    Purpose

    Mammalian expression of SOD1E133insTT.

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    E133insTT (after GA)

    Promoter

    EF-1α

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLEX_307-SOD1E133Δ

    Plasmid

    #83445

    Purpose

    Mammalian expression of SOD1E133Δ.

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    E133Δ

    Promoter

    EF-1α

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSOD1E133K-AcGFP1

    Plasmid

    #83442

    Purpose

    Expresses SOD1E133K in mammalian cells.

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    E133K

    Promoter

    CMV

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLEX307_CR-PDHA2S291A/S293A

    Plasmid

    #115205

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A/S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S291A/S293A (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pENTR223-PRDX5K85R

    Plasmid

    #98694

    Purpose

    Gateway cloning of human PRDX5-K85R

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    PRDX5 (PRDX5 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    K85R

    Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pENTR223-PRDX5K83R

    Plasmid

    #98693

    Purpose

    Gateway cloning of human PRDX5-K83R

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    PRDX5 (PRDX5 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    K83R

    Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pENTR223-PRDX5E80K

    Plasmid

    #98692

    Purpose

    Gateway cloning of human PRDX5-E80K

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    PRDX5 (PRDX5 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    E80K

    Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLEX_307-SOD1E133K

    Plasmid

    #83447

    Purpose

    Mammalian expression of SOD1E133K

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    E133K

    Promoter

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSOD1E133insTT-AcGFP1

    Plasmid

    #83441

    Purpose

    Expresses SOD1E133insTT in mammalian cells.

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    E133insTT

    Promoter

    CMV

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSOD1E133Δ-AcGFP1

    Plasmid

    #83419

    Purpose

    Expresses SOD1E133Δ in mammalian cells.

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    E133 deletion

    Promoter

    CMV

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pQP-iRIS

    Plasmid

    #102584

    Purpose

    Regulation of protein localization (near-infrared-blue-light-inducible shuttle)

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

    Insert

    BphP1-mVenus-CAAX-IRES2-NES-mCherry-QPAS1-AsLOV2cNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pPHAGE_DLD-H450A-C-TAP

    Plasmid

    #83478

    Purpose

    Mammalian expression of the dehydrogenase-deficient mutant DLD-H450A.

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    DLD (DLD Human)
    

    Use

    Lentiviral

    Tags

    TAP

    Expression

    Mammalian

    Mutation

    H450A

    Promoter

    CMV

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPHAGE_DLD-S456A-C-TAP

    Plasmid

    #83479

    Purpose

    Mammalian expression of the proteolysis-deficient mutant DLD-S456A.

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    DLD (DLD Human)
    

    Use

    Lentiviral

    Tags

    TAP

    Expression

    Mammalian

    Mutation

    S456A

    Promoter

    CMV

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLD-puro-Cc-PRDX5WT-VA

    Plasmid

    #98688

    Purpose

    Lentiviral expression of human PRDX5-WT in mammalian cells

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    PRDX5 (PRDX5 Human)
    

    Use

    Lentiviral

    Tags

    VA tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLD-puro-Cc-CR-PARK7WT-VA

    Plasmid

    #115187

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7WT into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PARK7WT (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Expression

    Mutation

    c.150G>A (silent when translated to protein, c…

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLD-puro-Cc-CR-PARK7V51G-VA

    Plasmid

    #115188

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7V51G into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PARK7V51G (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Expression

    Mutation

    c.150G>A (silent when translated to protein, c…

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLD-puro-Cc-CR-PARK7C53A-VA

    Plasmid

    #115189

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7C53A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PARK7C53A (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Expression

    Mutation

    c.150G>A (silent when translated to protein, c…

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLD-puro-Cc-CR-PARK7H126A-VA

    Plasmid

    #115190

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7H126A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PARK7H126A (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Expression

    Mutation

    c.150G>A (silent when translated to protein, c…

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLD-puro-Cc-CR-PARK7E163K-VA

    Plasmid

    #115191

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7E163K into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PARK7E163K (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Expression

    Mutation

    c.150G>A (silent when translated to protein, c…

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLD-puro-Cc-PRDX5K85R-VA

    Plasmid

    #98691

    Purpose

    Lentiviral expression of human PRDX5-K85R in mammalian cells

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    PRDX5 (PRDX5 Human)
    

    Use

    Lentiviral

    Tags

    VA tag

    Expression

    Mammalian

    Mutation

    K85R

    Promoter

    CMV

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLD-puro-Cc-PRDX5E80K-VA

    Plasmid

    #98689

    Purpose

    Lentiviral expression of human PRDX5-E80K in mammalian cells

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    PRDX5 (PRDX5 Human)
    

    Use

    Lentiviral

    Tags

    VA tag

    Expression

    Mammalian

    Mutation

    E80K

    Promoter

    CMV

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLD-puro-Cc-PRDX5K83R-VA

    Plasmid

    #98690

    Purpose

    Lentiviral expression of human PRDX5-K83R in mammalian cells

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    PRDX5 (PRDX5 Human)
    

    Use

    Lentiviral

    Tags

    VA tag

    Expression

    Mammalian

    Mutation

    K83R

    Promoter

    CMV

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pLSC-5

    Plasmid

    #62889

    Purpose

    Lenti vector for expression of inducible split Cas9

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Inserts

    SpCas9(574-1368)
    SpCas9(2-573)
    

    Use

    CRISPR and Lentiviral

    Tags

    FFBP12, FRB, NES PTK2, NLS SV40, and P2A

    Expression

    Mammalian

    Mutation

    *see comment below

    Promoter

    EFS

    Available since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. PX855

    Plasmid

    #62887

    Purpose

    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Aspartic acide 10 to Alanine (D10A)

    Promoter

    CBh

    Available since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pcDNA3-Cyto-GCaMP3

    Plasmid

    #64853

    Purpose

    Cytosol-targeted version of the Ca2+ indicator GCaMP3.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    GCaMP3
    

    Use

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover

    Plasmid

    #196829

    Purpose

    Express dpdpCas13b with AAAA mutation and Clover

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Insert

    dpspCas13b(AAAA)
    

    Use

    Tags

    3XFLAG and NES

    Expression

    Mammalian

    Mutation

    K367A, D369A and K370A

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pTL

    Plasmid

    #203176

    Purpose

    Centromeric yeast expression vector, leucine selection

    Depositor

    William Sessa

    Article

    Galosi et al Brain. 2022 Mar 29;145(1):208-223. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pcDNA3.1(+)-Cyto-CaNARi

    Plasmid

    #64849

    Purpose

    Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Use

    Tags

    Cerulean, Nuclear export signal (NES), and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge