We narrowed to 4,947 results for: mcs

1. pVETL-Cmcs-Flag-Ataxin3Q80-C14A

   Plasmid

   #22121

   Depositor

   Henry Paulson

   Article

   Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C14A

   Available Since

   Nov. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA

   Plasmid

   #133339

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_ctrA
   

   Use

   CRISPR

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT2KXIGdeltaIn-MCS-Avi-EGFP-rpl10a

   Plasmid

   #58380

   Purpose

   tol2 transgenesis for biotin based TRAP - Avi tagged Ribosome

   Depositor

   Didier Stainier

   Article

   Housley et al Development. 2014 Oct;141(20):3988-93. d

   Insert

   avi-EGFP-rpl10a
   

   Tags

   Avi Tag and EGFP

   Available Since

   Aug. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDSP15 (5’Δ HygR + loxP + MCS + 5’Δ mScarlet-I)

   Plasmid

   #193853

   Purpose

   Promoterless insertion vector for split HygR/mScarlet-I landing pad

   Depositor

   Patrick Phillips

   Article

   Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

   Insert

   5' rps-0::HygR + 5' mScarlet-I
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Promoter

   rps-0p

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE2-EF1a-MCS-hPOT1_IRES-blast

   Plasmid

   #166411

   Purpose

   express hPOT1 in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

   Insert

   hPOT1 (POT1 Human)
   

   Use

   Lentiviral

   Tags

   myc

   Expression

   Mammalian

   Promoter

   ef-1a

   Available Since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+) Laccase2 MCS-ciRS7 Exon

   Plasmid

   #69900

   Purpose

   Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ciRS7 (stw, CDR1-AS Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. UBQ10:sXVE:(MCS)-3xHA-S11

   Plasmid

   #108180

   Purpose

   Binary vectors used for the inducible expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

   Depositor

   Tzu-Yin Liu

   Article

   Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   UBQ10:sXVE:

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-TgKI-MCS-mLanYFP-polyA

   Plasmid

   #174021

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   mLanYFP
   

   Use

   Zebrafish knock-in tagging

   Available Since

   Nov. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPuroR-MCS-LexA-VP16-MiniWhite

   Plasmid

   #165898

   Purpose

   Puromycin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Selectable Empty Enhancer Driver
   

   Use

   Synthetic Biology

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-D-TOPO-PAmCherry1_160-236-MCS

    Plasmid

    #60546

    Purpose

    Cloning PAmCherry1 C-terminal fragment (RC) for BiFC-PALM

    Depositor

    Xiaolin Nan

    Article

    Nickerson et al PLoS One. 2014 Jun 25;9(6):e100589. doi:

    Insert

    PAmCherry1 C-terminal fragment
    

    Use

    Cloning

    Mutation

    amino acids 160-236

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-D-TOPO-PAmCherry1_1-159-MCS

    Plasmid

    #60545

    Purpose

    Cloning PAmCherry1 N-terminal fragment (RN) for BiFC-PALM

    Depositor

    Xiaolin Nan

    Article

    Nickerson et al PLoS One. 2014 Jun 25;9(6):e100589. doi:

    Insert

    PAmCherry1 N-terminal fragment
    

    Use

    Cloning

    Mutation

    amino acids 1-159

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA

    Plasmid

    #133342

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_ctrA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJEP307-pAAV-EFS(No AgeI)-MCS3-pA

    Plasmid

    #113684

    Purpose

    EFS driven Multi Cloning Site-3 without the AgeI restriction enzyme site.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    N/A
    

    Use

    AAV

    Promoter

    Cytomegalo Virus(CMV)

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMY-MCS-P2A-BlastR-DYK

    Plasmid

    #163357

    Purpose

    Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Blasticidin selection marker

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+) Laccase2 MCS-HIPK3 Exon

    Plasmid

    #69899

    Purpose

    Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    HIPK3 (stw Fly, Human)
    

    Expression

    Mammalian

    Mutation

    Expresses aa1-194 of HIPK3

    Promoter

    CMV

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

    Plasmid

    #133340

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_gcrA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMT-MCS-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80059

    Purpose

    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

    Plasmid

    #133334

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_ctrA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Hy_pMT Laccase2 MCS-WT Split GFP

    Plasmid

    #69891

    Purpose

    Expresses a GFP circular RNA in Drosophila

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    GFP, laccase2 (stw Fly)
    

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDF1-MCS2-EF1-Puro-RGS7

    Plasmid

    #113728

    Purpose

    Express RGS7 in mamalian cells

    Depositor

    Yardena Samuels

    Article

    Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

    Insert

    RGS7 (RGS7 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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