We narrowed to 5,570 results for: SPI

1. 3xNLS-NLP-cMyc-cMyc LbaCas12a

   Plasmid

   #182125

   Purpose

   pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   3xNLS-NLP-cMyc-cMyc LbaCas12a
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)

   Plasmid

   #114103

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO3) for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Petersen et al Front Neurol. 2022 Jan 20;12:792643. doi

   Insert

   sbGLuc-VChR1-EYFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   motoneuron-specific Homeobox 9 promoter

   Available since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJM1 flag-cFLIP

   Plasmid

   #211529

   Purpose

   Expresses flag-tagged cFLIP (CFLAR)

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   cFLIP (CFLAR Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)

   Plasmid

   #127392

   Purpose

   Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)

   Depositor

   Ryohei Yasuda

   Article

   Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   changed Threonine 286 to Aspartic acid and Threon…

   Promoter

   pCAG

   Available since

   July 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. CRY-Gal∆DD (B1013)

   Plasmid

   #92035

   Purpose

   Expresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRES

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fusion of plant CRY2 to Gal4 binding domain (AA1-…

   Promoter

   Available since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. CIB-VP64 (B1016)

   Plasmid

   #92037

   Purpose

   Expresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CIB1-VP64 (CIB1 Mustard Weed)
   

   Use

   Tags

   Fusion of plant CIB1 with VP64 activation domain.…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCF140_Gag-SpyCas9-NLS

   Plasmid

   #225959

   Purpose

   CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   CMV-Intron-Gag-SpyCas9-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV hUbC-dCas9-dMQ1-EGFG

   Plasmid

   #89794

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR and Lentiviral

   Tags

   3*FLAG and T2A-EGFP

   Expression

   Mammalian

   Mutation

   C141S, TGC-->TCC; S317A, AGC-->GCC

   Promoter

   hUbC

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. retro-puro vector

   Plasmid

   #58250

   Purpose

   Retroviral expression vector encoding an empty IRES-Puromycin cassette

   Depositor

   Georgios Stathopoulos

   Article

   Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTwist-WIV1-CoV Δ18

    Plasmid

    #164439

    Purpose

    Encodes WIV1-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    WIV1-CoV Spike truncated to remove 18 amino acids
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cx32R220stop

    Plasmid

    #78151

    Purpose

    Mammalian Expression of the first 219 amino acids of Connexin 32 and EGFP from a bicistronic mRNA

    Depositor

    Mario Bortolozzi

    Article

    Carrer et al Hum Mol Genet. 2017 Oct 25. pii: 4565496

    Insert

    Connexin 32 (GJB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Only contains first 219 residues

    Promoter

    CMV

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pRecLV105

    Plasmid

    #107238

    Purpose

    A lentiviral vector for expression in mammalian cells

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. W118-1_hZBTB7B

    Plasmid

    #180380

    Purpose

    lentiviral transduction of human ZBTB7B gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    ZBTB7B (ZBTB7B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT2-TetR-neoR

    Plasmid

    #153310

    Purpose

    Tet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GW

    Depositor

    Spyros Petrakis

    Article

    Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

    Insert

    TetR
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC

    Plasmid

    #140197

    Purpose

    Episomal expression of dLbCas12a-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Insert

    dLbCas12a-VPR
    

    Use

    CRISPR and Synthetic Biology; Expression in asper…

    Tags

    VPR (VP64-p65-Rta), NLS

    Expression

    Mutation

    D832A DNase deactivated

    Promoter

    PgpdA

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ230-RR

    Plasmid

    #108860

    Purpose

    LbCpf1 Gateway entry plasmid with G548R and K611R mutations

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

    Insert

    LbCpf1-RR (LbCpf1 with G548R and K611R mutations)
    

    Use

    CRISPR; Gateway compatible cpf1 entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    G548R and K611R, Cpf1 is rice codon optimized

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSUMO-β′-COPI-WD40

    Plasmid

    #180753

    Purpose

    Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40 (residues 1-301).

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    β′-COPI-WD40 (SEC27 Budding Yeast)
    

    Use

    Tags

    N-terminal strep tag, His-tag, and SUMO tag

    Expression

    Bacterial

    Mutation

    Encodes residues 1-301; deleted 302-889; mutated …

    Promoter

    T7

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBRY-nuclear mCherry-IRES-PURO

    Plasmid

    #52409

    Purpose

    Constitutively expressed mammalian expression vector encoding nuclear mCherry fluorescent reporter. Puromycin resistance.

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    nuclear mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNOC_hlbCas12a-Nlux

    Plasmid

    #176240

    Purpose

    pNOC episomal plasmid harboring the humanized lbCas12a gene sequence tagged with Nlux under the control of Ribi promoter

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized lbCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Ribosomal subunit

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-MTS-CA-c-Src-FLAG

    Plasmid

    #44654

    Depositor

    Yoshimi Homma

    Article

    Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

    Insert

    v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
    

    Use

    Tags

    FLAG tag and mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    changed Tyr 530 to Phe

    Promoter

    CMV promoter

    Available since

    May 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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