177,138 results
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Plasmid#182972PurposeStably express CD63 fused with mScarlet in mammalian cells via the sleeping beauty transposon system.DepositorAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pcDNA3.1-mBeRFP
Plasmid#175173Purposelarge-stokes shift red fluorescent protein mBeRFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmBeRFP
Available sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRBC-sfGFP-150TAG
Plasmid#174076PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,DepositorInsertSuperfolder GFP
TagsHis-6ExpressionBacterialMutationN150TAGPromoterT7Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRPN265NB-LaG16-MNase
Plasmid#170978PurposeExpresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & RunDepositorInsertGlutathione-S-Transferase/LaG16/MNase
TagsGSTExpressionBacterialAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-ChR2-YFP
Plasmid#171622PurposeTRE-ChR2-YFP reporterDepositorInsertTRE-ChR2-YFP
UseAAV and Synthetic BiologyExpressionMammalianPromoterTetOAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU294
Plasmid#167689PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::Cas9i
UseCRISPRAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Sst44-GFP
Plasmid#172310PurposeSST interneuron-restricted gene regulatory element GRE44, to drive GFP expression in SST+ interneuronsDepositorInsertSst44
UseAAVPromoterpB-GlobinAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRecT_2
Plasmid#167546PurposeEncodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.DepositorInsertE. faecium Com15 recT under an IPTG inducible promoter, with lacI
ExpressionBacterialPromoterIPTGAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GfaABC1D BARK p2A mCherry
Plasmid#117691PurposeGfaABC1D-iBARK-p2A-mCherry: Viral expression vector for astrocyte Gaq silencingDepositorInsertBARKrgs peptide
UseAAVTagsHA / FLAGAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV_hSynapsin_psychLight2
Plasmid#163909PurposeTo generate Adeno-Associated viruses for expression of psychLight2 under synapsin promoterDepositorHas serviceAAV9InsertpsychLight2
UseAAVTagsFlag tagExpressionMammalianPromoterSynapsinAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-sgRNA-RFP
Plasmid#166005PurposeBacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)DepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-3xFLAG-GFP-puroR
Plasmid#167463Purpose3rd-gen lentiviral expression of FLAG-GFP in mammalian cellsDepositorInsertGFP
UseLentiviralTags3xFLAGExpressionMammalianPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMOS016: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)
Plasmid#163058PurposeroGFP2-Orp1 H2O2 oxidation sensor (cytosolic)DepositorInsertroGFP2-Orp1
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTRE3G-NlucP
Plasmid#162595PurposeC-terminally PEST-tagged NanoLuc under TRE3G promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNanoLuc-PEST
TagsPESTExpressionMammalianPromoterTRE3G promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSL21-mCherry
Plasmid#164410PurposeRetroviral expression plasmid of sgRNA with mCherryDepositorTypeEmpty backboneUseCRISPR and RetroviralExpressionMammalianPromoterU6 promoter for crRNA expression and EFS promoter…Available sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.GFAP.(Cyto)iGlucoSnFR
Plasmid#164513PurposeGreen glucose sensor, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2 (Plasmid #244111) instead of this plasmid.DepositorInsert(Cyto)iGlucoSnFR
UseAAVMutationSynthetic ConstructPromoterGFAPAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRE:mCherry-p2a- bReaChES
Plasmid#163037PurposeFLiCRE reporter gene for neuronal expressionDepositorInsertmCherry-p2a-bReaChES
UseAAVExpressionMammalianPromoterTREAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSEM238
Plasmid#161515PurposeNeuronal expression of histamine-sensitive chloride channel for negative selectionDepositorInsertpEXP[Psnt-1 | histamine | rpl-3 UTR]
ExpressionWormPromoterPsnt-1Available sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JD639
Plasmid#160398PurposeBinary vector pGWB14 including miR396-resistant Vitis GRF4-GIF1 under 35S promoter/Plant transformationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert35S::vitis miR396-resistant GRF4-GIF1 chimera
UseBinary vectorAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mGreenLantern
Plasmid#161913PurposemGreenLantern fluorescent protein: bacterial expression plasmidDepositorInsertmGreenLantern
Tags6xHisExpressionBacterialPromoteraraBAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK262 (RAD21-mAC Neo)
Plasmid#140538PurposeRAD21 tagging with mAID-CloverDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRAD21 (RAD21 Human)
UseCRISPRExpressionMammalianAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK395 (AAVS1 CMV-OsTIR1(F74A))
Plasmid#159992PurposeExpression of OsTIR1(F74A) in human cellsDepositorInsertCMV-OsTIR1(F74A)
ExpressionMammalianAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX303-ABI1-dCas9
Plasmid#154474PurposeExpresses ABI1 fused to the N terminus of dCas9DepositorInsertABI1 (ABI1 Mustard Weed)
UseLentiviralExpressionMammalianMutationIncludes ABI1 aa 126-423, D143APromoterCMVAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mCherry
Plasmid#155222PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
TagsmCherryExpressionMammalianAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-FOXP1
Plasmid#153145PurposeExpression of Flag tagged human FOXP1 in mammalian cellsDepositorAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLEX307-APN-puro
Plasmid#158454PurposeConstitutive lentiviral expression of human APN.DepositorAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαq-LgB97
Plasmid#134360PurposeNanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGαq-LgB97 (GNAQ Human)
TagsHAExpressionBacterial and MammalianPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AIMTOR A757
Plasmid#140829PurposeAIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAIMTOR A757 (ULK1 Human)
TagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of hum…Available sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
P307-FBP_6
Plasmid#139697PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathwayDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
UseLuciferaseExpressionPlantPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.b201
Plasmid#131404PurposeE. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albuminDepositorInsertNanobody b201
Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-AckRS
Plasmid#137976Purposesequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansionDepositorInsertAckRS and pylTcua
ExpressionBacterialPromoteraraBADAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pH2B-emiRFP703
Plasmid#136567PurposeHistone 2B labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B-emiRFP703
TagsemiRFP703ExpressionMammalianPromoterCMVAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
M3-M14-dCas9
Plasmid#134779PurposeExpression of fusion protein M3-M14-dCas9 in Mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertExpressionMammalianPromoterCMVAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC-GFP-AT
Plasmid#133306Purpose"Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisationDepositorInsertaxe/txe toxin-antitoxin cassette
UseSynthetic BiologyExpressionBacterialAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pORANGE Gria1-GFP KI
Plasmid#131489PurposeEndogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 acGFP
Plasmid#128047PurposeMammalian expression vector with N terminal AcGFPDepositorTypeEmpty backboneTagsAcGFPExpressionMammalianAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
scOrange chromoprotein
Plasmid#117840PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses scOrange chromoprotein in E. coliDepositorInsertpromoter, RBS, scOrange
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDonor_AcCAST_kanR
Plasmid#127925PurposeKanamycin resistance donor for AcCASTDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKanR
Available sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRZero
Plasmid#120275PurposeBlunt-end cloning vector of PCR-products using ZERO-backgound systemDepositorTypeEmpty backboneAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRT
Plasmid#120274PurposeTA-cloning vector of PCR-productsDepositorTypeEmpty backboneAvailable sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB
Plasmid#122668PurposeC terminal domain of hnRNPA1 (186-320) tagged with SspB and mCherry.DepositorInsertHNRNPA1 (HNRNPA1 Human)
UseLentiviralTagsmCherry-SspBExpressionMammalianMutationContains only amino acids 186-230PromoterSFFVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGTag-gal4-VP16-B-actin
Plasmid#117817PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgal4/VP16
UseSynthetic BiologyAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-ROR1
Plasmid#116789PurposeLentiviral expression of ROR1DepositorAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ALK
Plasmid#116712PurposeLentiviral expression of ALKDepositorAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-KRAS-G12V
Plasmid#116424PurposeLentiviral expression of KRAS G12VDepositorAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK348 (BSD-P2A-mAID-mClover)
Plasmid#121182PurposeN-terminal taggingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBSD-P2A-mAID-mClover
UseCRISPRAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMP71-HLA-A0201-His
Plasmid#108214PurposeHLA-A0201 his taggedDepositorInsertmajor histocompatibility complex, class I, B (HLA-A Human)
UseRetroviralTagshisExpressionMammalianAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
2Flag-pDEST-N
Plasmid#118371PurposeGateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2FlagExpressionMammalianAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBA-mCherry-EGFP-PIM
Plasmid#111758PurposeLow level expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 additionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
ExpressionMammalianMutationVal24Glu, Tyr80Cys, Ala95Thr mutations in second …Promoterchicken beta-actinAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti6.2_TagBFP
Plasmid#113724PurposeExpresses the fluorescent protein TagBFP in a lentiviral backboneDepositorInsertTagBFP
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by