176,532 results
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pLCRcala (p53)
Plasmid#12136DepositorInsertp53 (Trp53 Mouse)
ExpressionMammalianAvailable sinceSept. 21, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-myctagged MyoD
Plasmid#8399DepositorAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSKI074
Plasmid#11571DepositorInsertCaMV 35S enhancers
UseActivation taggingExpressionPlantAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBABE hygro MEN1 WT
Plasmid#11024DepositorAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Stat1 alpha pRC/CMV
Plasmid#8690DepositorInsertSTAT1 alpha (STAT1 Human)
ExpressionMammalianAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
1432 pcDNA3 flag MAGI1c
Plasmid#10714DepositorAvailable sinceSept. 28, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
OpenCRISPR-1
Plasmid#221565PurposeExpress OpenCRISPR-1 nuclease in human cells using a CMV promoterDepositorInsertOpenCRISPR-1
TagsNLSExpressionMammalianPromoterCMVAvailable sinceAug. 28, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
SpCas9-CBE6b
Plasmid#215820PurposeExpress CBE6b (with SpCas9) in mammalian cellsDepositorInsertCBE6b-SpCas9-2xUGI (cas9 )
ExpressionMammalianAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKeima-LiveDrop
Plasmid#199695Purposelipophagy reporter system based on the Keima-fluorophoreDepositorAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV enAsCas12f-HKRA-sgRNA_v7
Plasmid#208357PurposeExpression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cellsDepositorInsertenAsCas12f-HKRA
ExpressionMammalianMutationI123H/D195K/D208R/V232APromoterCMVAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAF0687 Pa01_attP-mCherry donor
Plasmid#193466PurposePromoterless mcherry donor for Pa01DepositorInsertPa01_attP
ExpressionMammalianAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRC0940 (circRNA entry vector)
Plasmid#189967PurposeCloning vector for generating template plasmids to transcribe circRNA, with mNeonGreen dropout following BsaI digestionDepositorInsertmNeonGreen
ExpressionMammalianPromoterT7Available sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmKappa-Tn5
Plasmid#184286PurposeAnti-mouse kappa chain specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmKappa-Tn5
Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB_iETV2_P2A_GFP_Puro
Plasmid#168805PurposePiggyBac transposon construct with doxycycline inducible ETV2 + GFP expressionDepositorInsertETV2 (ETV2 Human, Synthetic)
UseSynthetic Biology; PiggybacExpressionMammalianPromoterTREAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Gs(short)-CASE
Plasmid#168124PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).DepositorUseLuciferaseTagscpVenus on GNG1ExpressionMammalianMutationNLuc is inserted at N136/V137 within GNASAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Gq-CASE
Plasmid#168125PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at F124/E125 within GNAQAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP
Plasmid#158962PurposeCre-dependent Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed DsRed cassette
Cas9-GFP
UseCRISPR and Cre/LoxAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-PD-L1
Plasmid#121492PurposeExpression of human PD-L1 WT in mammalian cellsDepositorAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSIE1
Plasmid#136355PurposeSelection by Inducible EffectorsDepositorInsertsType VI effector bfe1
Type VI effector bte1
BT_4676
tetR
UseUnspecifiedMutationATG removed to add signal sequence before genePromoterP1TDP-GHO23Available sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMH-Halo tag
Plasmid#154144PurposeGateway compatible mammalian expression plasmid for halo tag fusion proteinsDepositorTypeEmpty backboneTagsHalo tagExpressionMammalianAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p3xFLAG SARS-CoV2 RdRp.co
Plasmid#154008PurposeExpression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian cellsDepositorInsertCodon-optimized SARS-CoV2 Nsp12 from ORF1ab (ORF1ab Synthetic)
Tags3xFLAG tagExpressionMammalianPromoterCMVAvailable sinceJune 23, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLentiV2-dCas9-VP64
Plasmid#141104PurposeExpression of gRNA and dCas9-VP64 from the same plasmid for CRISPRaDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-sACE2(WT)-8his
Plasmid#149268PurposeMammalian expression plasmid for his-tagged sACE2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR207 SARS-CoV-2 ORF7A
Plasmid#141276PurposeGateway-compatible Entry vector, with insert of ORF7A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertORF7A (ORF7a SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
YE1-BE4max-NG
Plasmid#138159PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E; within Cas9 D10A + …PromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NW30
Plasmid#133444PurposeCovalently circularized nanodisc NW30 for studying membrane proteins and viral entry.DepositorInsertNW30
ExpressionBacterialAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE
Plasmid#131004Purposebicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoterDepositorHas serviceAAV8InsertChRmine
UseAAVTagsKv2.1-HAPromoterhSynAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBECAb-apr
Plasmid#122001PurposeA cytidine deaminase-mediated base-editing plasmid in Acinetobacter baumanniiDepositorTypeEmpty backboneUseCRISPRAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHelper_ShCAST
Plasmid#127922PurposeExpresses ShCAST and a crRNA targeting PSP1DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k
Available sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by
