164,881 results

1. ilux pGEX(-)

   Plasmid

   #107879

   Purpose

   Bacterial expression of ilux for bioluminescence imaging

   Depositor

   Stefan Hell

   Article

   Gregor et al Proc Natl Acad Sci U S A. 2018 Jan 30;11

   Insert

   ilux
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC1-V16-hGDNF

   Plasmid

   #122035

   Purpose

   AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and hGDNF expression

   Depositor

   Liliane Tenenbaum

   Article

   Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

   Insert

   hGDNF (GDNF Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21 MBPx-SpyCatcher

   Plasmid

   #72327

   Purpose

   Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   pET21 MBPx-SpyCatcher
   

   Use

   Tags

   N-terminal His6 tag

   Expression

   Bacterial

   Mutation

   MBP contains A312V, I317V mutations and 172, 173,…

   Promoter

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-DsRed

   Plasmid

   #11151

   Purpose

   Mammalian expression vector for expression of DsRed

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Has Service

   Cloning Grade DNA

   Insert

   DsRed2 from Discosoma sp
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-DIO ChETA-EYFP

   Plasmid

   #26968

   Purpose

   AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   hChR2(E123T/H134R)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T, H134R

   Promoter

   EF1alpha

   Available Since

   Jan. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGW-GOI-TurboID

   Plasmid

   #209378

   Purpose

   Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn1-mTurquoise2

   Plasmid

   #99125

   Purpose

   An AAV genome that expresses the fluorescent protein mTurquoise2 from the hSyn1 promoter

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   mTurquoise2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. hE-cadherin/Δp120

   Plasmid

   #45770

   Depositor

   Barry Gumbiner

   Article

   Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

   Insert

   hE-cadherin/Δp120 (CDH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amino acids 762-764 converted from EED to AAA to …

   Promoter

   CMV

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

9. CIBN-CAAX

   Plasmid

   #79574

   Purpose

   expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting

   Depositor

   Pietro De Camilli, Olof Idevall-Hagren

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   CIBN (CIB1 Mustard Weed)
   

   Use

   Tags

   C-terminal CAAX-box

   Expression

   Mammalian

   Mutation

   K93A, R94A, K106A, K107A (please see depositor co…

   Promoter

   CMV

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDB5318

    Plasmid

    #204828

    Purpose

    An integrating plasmid containing both the inducible enotetS promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

    Depositor

    Li-Lin Du

    Article

    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

    Inserts

    tetR
    mECitrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CMV promoter and enotetS promoter

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMaCTag-P07

    Plasmid

    #124788

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreen

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mNeonGreen
    

    Use

    Pcr template

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    None

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAG-mCherry

    Plasmid

    #108685

    Purpose

    Strong CAG promoter with mCherry expression for transfection efficacy screening

    Depositor

    Jordan Green

    Article

    Mishra et al Regen Eng Transl Med. 2019 Sep;6(3):273-

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTight-hMYT1L-N174

    Plasmid

    #31877

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

    Insert

    myelin transcription factor 1-like (MYT1L Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1+-mutTornado-split-nLuc

    Plasmid

    #212707

    Purpose

    Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

    Depositor

    Samie Jaffrey

    Article

    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

    Insert

    mutTornado-CVB3-split-nLuc
    

    Use

    Tags

    Expression

    Mutation

    Deleted the partial 3'ribozyme sequence in T…

    Promoter

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-APOBEC1-YTH

    Plasmid

    #131636

    Purpose

    Expresses APOBEC1 fused to the YTH domain

    Depositor

    Kate Meyer

    Article

    Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

    Insert

    APOBEC1 fused to YTH domain (YTHDF2 Human, Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-Wnt7B

    Plasmid

    #35915

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt7B (Wnt7b Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mCherry-hLC3B-pcDNA3.1

    Plasmid

    #40827

    Depositor

    David Rubinsztein

    Article

    Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

    Insert

    LC3B (MAP1LC3B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223_SRC_WT

    Plasmid

    #82165

    Purpose

    Gateway Donor vector containing SRC , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SRC (SRC Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAnc80L65AAP

    Plasmid

    #92307

    Purpose

    Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAP

    Depositor

    Luk Vandenberghe

    Article

    Landegger et al Nat Biotechnol. 2017 Mar;35(3):280-284.

    Insert

    Ancestral AAV Capsid Anc80L65AAP
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Rep2

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cterminal HA WDR59 pRK5

    Plasmid

    #46328

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    WDR59 (WDR59 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. EF1a_FOXA2_P2A_Hygro_Barcode

    Plasmid

    #120439

    Purpose

    Barcoded lentiviral vector to express FOXA2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    FOXA2 (FOXA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCMVβ-p300-myc

    Plasmid

    #30489

    Purpose

    Mammalian expression of human p300 with myc tag

    Depositor

    Tso-Pang Yao

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTP1112

    Plasmid

    #104158

    Purpose

    Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)
    

    Use

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

    Plasmid

    #46168

    Purpose

    Codon optimized Cas9_SV40 NLS with intron

    Depositor

    John Calarco

    Article

    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

    Insert

    codon optimized Cas9_SV40 NLS with intron
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    intron inserted at position 3113-3163, and SV40 N…

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBS130

    Plasmid

    #26290

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    phiC31 integrase (int Streptomyces phage phiC31)
    

    Use

    Drosophila transgenesis

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available Since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. myrAkt delta4-129

    Plasmid

    #10841

    Depositor

    Richard Roth

    Article

    Kohn et al J Biol Chem. 1996 Sep 6. 271(36):21920-6

    Insert

    Akt (AKT1 Human)
    

    Use

    Tags

    HA and myr

    Expression

    Mammalian

    Mutation

    Deletion of PH domain (aa4-129)

    Promoter

    SV40

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTK_Citrine_Nanotag_24_positive_Control

    Plasmid

    #130570

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    FoxD3_cranial_neural_crest_enhancer_NC1
    

    Use

    Enhancer cloning nanotag reporter vector

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CL20_mEGFP_PAX3_FOXO1

    Plasmid

    #205875

    Purpose

    Express mEGFP-tagged fusion protein, PAX3_FOXO1 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    PAX3_FOXO1 (PAX3, FOXO1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Flag-ZDHHC7

    Plasmid

    #231656

    Purpose

    Expresses human ZDHHC7 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC7 (ZDHHC7 Human)
    

    Use

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. YFP-NOP58-C1

    Plasmid

    #134537

    Purpose

    Expresses YFP-NOP58 fusion protein

    Depositor

    Susan Janicki

    Article

    Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

    Insert

    NOP58 (NOP58 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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