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pAAV-hSyn-DIO-GRAB_ACh3.0

Plasmid

#121923

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

Insert

GPCR activation based ACh sensor GRAB-ACh3.0

Use

AAV

Promoter

hSyn

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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TDP-43 WT GFP

Plasmid

#237542

Purpose

Expression of GFP-tag fused to wild-type TDP-43

Depositor

Tatyana Shelkovnikova

Article

Matsukawa et al RNA Biol. 2021 Jan 11:1-9. doi: 10.1080/

Insert

GFP-tagged TDP-43 WT (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

wild-type TDP-43

Promoter

CMV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3_GLP1R-NTEV-TCS-GV-2xHA

Plasmid

#194378

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

GLP1R-NTEV-TCS-GV-2xHA (GLP1R Human, Synthetic)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE smFP-V5 donor ORF2

Plasmid

#200382

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pFZ19

Plasmid

#36184

Purpose

Expression of genes under control of an estrogen-inducible 35S promoter

Depositor

Daniel Voytas

Article

Zhang et al Proc Natl Acad Sci U S A. 2010 Jun 29. 1

Type

Empty backbone

Use

Plant t-dna vector

Expression

Plant

Promoter

XVE_35S (estrogen-inducible)

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-TREX1(D18N)

Plasmid

#27220

Depositor

Judy Lieberman

Article

Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

Insert

TREX1 (TREX1 Human)

Tags

GFP

Expression

Mammalian

Mutation

D18N sequence 52-GAC changed to AAC, resulting i…

Available Since

Jan. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pCVD442

Plasmid

#11074

Depositor

Michael Donnenberg, James Kaper

Article

Donnenberg et al Infect Immun. 1991 Dec . 59(12):4310-7.

Insert

sacB (sacB Bacillus subtilis)

Expression

Bacterial

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Bcl11a

Plasmid

#139809

Purpose

doxycycline-inducible expression of human Bcl11a in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

BCL11a (BCL11A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-Rab9a

Plasmid

#78592

Purpose

Express mCherry-Rab9a in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

Rab9a (RAB9A Human)

Tags

mCherry2

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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hAAVS1 1L TALEN

Plasmid

#35431

Purpose

TALEN designed to target the human AAVS1 locus

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

hAAVS1 1L TALEN

Expression

Mammalian

Available Since

May 17, 2012

Availability

Academic Institutions and Nonprofits only

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TFORF2302

Plasmid

#142018

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CIITA (CIITA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgTh

Plasmid

#159901

Purpose

Mutagenesis of Th

Depositor

Larry Zweifel

Insert

Th (Th Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pmGFP-ADAR1-p150

Plasmid

#117927

Purpose

ADAR1-p150 expression plasmid.

Depositor

Kumiko Ui-Tei

Article

Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

Insert

ADAR1-p150 (ADAR Human)

Tags

myc-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)

Plasmid

#71814

Purpose

Expresses high specificity SpCas9. Px330-like plasmid.

Depositor

Feng Zhang

Article

Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

Has Service

Cloning Grade DNA

Insert

enhanced specificity Cas9 (1.1)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

K848A, K1003A, & R1060A

Promoter

CBh

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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Tol2pA2-drl:APEX2-mCherry

Plasmid

#188945

Purpose

Plasmid for Tol2 transgenesis expressing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry, driven by the draculin promoter

Depositor

Owen Tamplin

Article

Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 1

Insert

drl:mito-APEX2_p2A_APEX2-H2B_p2A_mCherry_polyA

Use

Tol2 destination vector (attr4-r3)

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pTRE-Slow-FT

Plasmid

#31915

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Slow-FT

Expression

Mammalian

Mutation

M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

Promoter

PTight Tet-responsive PminCMVd

Available Since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pNF02-mLychee

Plasmid

#219400

Purpose

Constitutive expression of mLychee in E. coli

Depositor

Christian Lesterlin

Article

Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

Insert

mLychee

Expression

Bacterial

Promoter

proDp

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-GshF-blasticidin

Plasmid

#210313

Purpose

Expresses humanized Streptococcus thermophilus GshF in mammalian cells

Depositor

Kivanc Birsoy

Article

Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

Insert

Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)

Use

Retroviral

Tags

FLAG peptide

Mutation

Codon optimized for expression in human cells

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pHiUGE smFP-V5 donor ORF0

Plasmid

#200380

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE smFP-V5 donor ORF1

Plasmid

#200381

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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PLEX-PGK-LAMP1-HaloTag

Plasmid

#164216

Purpose

pLEX lentivirus backbone expresses HaloTag tagged LAMP1 under PGK promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

HaloTag

Promoter

PGK

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-NFKB1

Plasmid

#139822

Purpose

doxycycline-inducible expression of Human NFKB1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

NFKB1 (NFKB1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-OTp-mCherry

Plasmid

#184754

Purpose

To drive mCherry under the control of mouse oxytocin promoter

Depositor

Kazunari Miyamichi

Article

Inada et al Neuron. 2022 Apr 12. pii: S0896-6273(22)

Insert

mCherry

Use

AAV

Promoter

mouse Oxytocin promoter

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLC-ZsGreen-P2A-Puro

Plasmid

#124302

Purpose

Lentiviral vector for expression of ZsGreen-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

ZsGreen

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pIKU801-CrPEPC1

Plasmid

#92342

Purpose

Expresses sgRNA for targeting CrPEPC1 gene, paromomycin resistance

Depositor

I-Son Ng

Article

Kao et al Bioresour Technol. 2017 May 4. pii: S096

Insert

sgRNA-CrPEPC1

Use

CRISPR

Expression

Plant

Promoter

AtU6-26

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDD428

Plasmid

#202081

Purpose

Cas9/sgRNA plasmid targeting the C-terminus of Myh9

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Myh9 CT sgRNA (Myh9 S. pyogenes (Cas9))

Use

CRISPR

Promoter

EF1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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MZB211

Plasmid

#228494

Purpose

10xHis-SUMO (Smt3) tagged Ssod7-fused Pfu Polymerase with fidelity enhancing mutation (E. coli based protein expression)

Depositor

Richard Baker

Insert

pol

Tags

10xHis-SUMO(Smt3)

Expression

Bacterial

Mutation

A408S

Promoter

T7 Promoter

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCLXEBR-pTF-kRasV12

Plasmid

#114318

Purpose

TET-inducible expression of the V12-mutant form of the ki-Ras protein

Depositor

Patrick Salmon

Insert

Ras (KRAS Human)

Use

Lentiviral

Mutation

G12V oncogenic mutation. Constitutively active K-…

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pQE30-His-CKM

Plasmid

#124134

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Creatine kinase (CKM Chicken)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBE14

Plasmid

#174653

Purpose

In vivo Cre-lox circularization in E. coli

Depositor

Huimin Zhao

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Insert

Cre

Use

Synthetic Biology

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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