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Showing: 7021 - 7050 of 158094 results

  1. Cas12aUltra_5XNLS

    Plasmid
    #218775
    Purpose
    His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS
    Depositor
    Ross Wilson
    Article
    Sahu et al bioRxiv 2024.07.14.603391
    Insert
    5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
    Use
    CRISPR
    Tags
    6XHis (C terminal), HA, Nucleoplasmin, SV40, and …
    Expression
    Bacterial
    Mutation
    Nucleotides mutations were made to encode for arg…
    Promoter
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    218775-plasmid-map-sequence-id-443448

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  8. pcDNA3.1-Pur-mEGFP

    Plasmid
    #186329
    Purpose
    Fluorescent reporter system
    Depositor
    Bertrand Collet
    Insert
    mEGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186329-plasmid-map-sequence-id-400611

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  10. pcDNA3.2-V5-DEST-hSFRP1-his

    Plasmid
    #189540
    Purpose
    Protein expression of SFRP1 in mammalian cells.
    Depositor
    Haian Fu
    Insert
    secreted frizzled related protein 1 (SFRP1 Human)
    Use
    Tags
    his
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    189540-plasmid-map-sequence-id-374109

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  14. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid
    #139987
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139987-plasmid-map-sequence-id-275335

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  24. pET29a-human histone 4-in-One

    Plasmid
    #223237
    Purpose
    Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4
    Depositor
    Weixing Zhao
    Inserts
    His6-VSVG-Histone H2A
    Histone H2B
    Histone H3
    Histone H4-TEV-His6-Dual StrepII
    Use
    Tags
    Dual StrepII, His6, TEV protease site, and VSVG
    Expression
    Bacterial
    Mutation
    A103G
    Promoter
    T7
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    223237-plasmid-map-sequence-id-443806

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  29. pOpen-MMLV_RT (lack H)

    Plasmid
    #165556
    Purpose
    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165556-plasmid-map-sequence-id-339910

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Showing: 7021 - 7050 of 158094 results