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164,881 results

Showing: 7021 - 7050 of 164881 results

  1. pHDR-USR-eGFP

    Plasmid
    #208830
    Purpose
    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed eGFP but without Cas9. The selective PuroR reporter gene was designed to be repaired only by the sgRNA/Cas9-triggered HDR.
    Depositor
    Zhiying Zhang
    Article
    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, U6
    Available Since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    208830-plasmid-map-sequence-id-414896

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  3. PCR4-HBGP::mCherry-SI-HBGP::eGFP-H11

    Plasmid
    #216394
    Purpose
    dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct (human beta-globin promoter) into the H11 locus (separated by a synthetic insulator)
    Depositor
    Evgeny Kvon
    Article
    Hollingsworth et al Nat Commun. 2025 Jan 6;16(1):409. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human beta-globin
    Available Since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216394-plasmid-map-sequence-id-427267

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  5. pcDNA5/FRT/TO-GAlphai1-RLuc8

    Plasmid
    #140973
    Purpose
    Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphai1-RLuc8 (GNAI1 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140973-plasmid-map-sequence-id-300655

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  7. Artichoke

    Plasmid
    #73320
    Purpose
    Artichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Benjamin Ebert
    Article
    Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and EF1a
    Available Since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    73320-plasmid-map-sequence-id-135028

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  8. pME-mTagBFP-CAAX

    Plasmid
    #75149
    Purpose
    Membrane targeted TagBFP (blue) fluorophore for use with 5’ promoters.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    mTagBFP-CAAX
    Use
    Middle entry vector for multisite gateway three f…
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Available Since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75149-plasmid-map-sequence-id-142103

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  13. pFL

    Plasmid
    #110739
    Purpose
    Plasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP site
    Depositor
    Tim Richmond
    Article
    Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin and p10 promoters
    Available Since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    110739-plasmid-map-sequence-id-213587

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  14. pMP71-super-IL-2

    Plasmid
    #174599
    Purpose
    Expresses extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) developed by the Garcia Lab
    Depositor
    Stanley Riddell
    Article
    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441
    Insert
    extracellular domain of huIL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    174599-plasmid-map-sequence-id-344458

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  19. pETDuet_huCAD_ICADL (TevS)

    Plasmid
    #100098
    Purpose
    dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences
    Depositor
    Jim Wells
    Article
    Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112
    Insert
    DNA fragmentation factor subunit alpha (CAD, DFFA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    two caspase cleavage sites (DETD-117 and DAVD-224…
    Promoter
    T7
    Available Since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    100098-plasmid-map-sequence-id-261413

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  22. mCherry-Lifeact-7

    Plasmid
    #54491
    Purpose
    Localization: Action, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mCherry-Lifeact
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    54491-plasmid-map-sequence-id-227430

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  23. pLJM1-B-GRAM-W-NeoR

    Plasmid
    #211710
    Purpose
    Lentiviral expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W)
    Depositor
    Yasunori Saheki
    Article
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Use
    Lentiviral
    Tags
    ddFP-B3
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Tryptophan
    Promoter
    CMV
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211710-plasmid-map-sequence-id-419208

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  28. pEGFP-C1-SopF

    Plasmid
    #137734
    Purpose
    Expresses EGFP fused to SopF
    Depositor
    Leigh Knodler
    Insert
    SopF
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    137734-plasmid-map-sequence-id-271547

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Showing: 7021 - 7050 of 164881 results