We narrowed to 82,306 results for: ELL
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Plasmid#221284PurposeExpression of RFP (pLxIS-PLPLR) in mammalian cells by retroviral transductionDepositorAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only
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pMSCV-FKBP-TRIF(Nterm)
Plasmid#221244PurposeExpression of TRIF N-terminus (1-222) in mammalian cells by retroviral transductionDepositorAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEND-303_Cacnes_tetR
Plasmid#225618PurposeC. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO2)+RBS_1+mCherryDepositorInsertTetR
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-192_Cacnes_HS
Plasmid#225621PurposeC. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS09160)+RBS_1+HspR(PPA_RS10230).DepositorInsertHspR
UseSynthetic BiologyTagspFAST-6XHisExpressionBacterialAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330_ATP1A1_G7_Dual_sgRNA
Plasmid#173206PurposeCoselection for HDR in human cells. Vector for dual expression of ATP1A1 G7 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.DepositorInsertATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9
UseCRISPRExpressionMammalianPromoterDual U6 promotersAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mScarlet-H2BC11
Plasmid#207756PurposeDonor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX330-PITCh-MYO1C
Plasmid#227305PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MYO1C for knock-in.DepositorInsertsgRNA Targeting N-terminus of MYO1C (MYO1C Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
HsCLYBL-FLAG
Plasmid#111290Purposemammalian expressionDepositorInserthuman CLYBL (CLYBL Human)
Tagslinker and FLAG tag: TGGGSGGSGGSMDYKDDDDKExpressionMammalianAvailable SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
HsCLYBL(D320A)-FLAG
Plasmid#111291Purposemammalian expressionDepositorInserthuman CLYBL D320A (CLYBL Human)
Tagslinker and FLAG tag: TGGGSGGSGGSMDYKDDDDKExpressionMammalianMutationD320AAvailable SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-GL
Plasmid#83342Purposefor Gateway cloning of promoter elements upstream of a GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsGFP::Luciferase(nls)ExpressionInsectAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNAIIIB cdc42 QL
Plasmid#61636Purposemammalian expression of cdc42 constitutively active mutantDepositorAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-PXN
Plasmid#227316PurposeDonor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)DepositorInsertPXN Homology Arms flanking a mStayGold Tag (PXN Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
CB6-GFP-TAOK2 (K57A)
Plasmid#197114PurposeExpression of kinase-deficient GFP-tagged TAOK2 (K57A) / PSK1 (K57A) in mammalian cellsDepositorInsertTAOK2 (K57A) (TAOK2 Human)
TagsGFPExpressionMammalianMutationChanged K 57 to APromoterCMVAvailable SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-TGFBR2-wt_3'UTR
Plasmid#31882DepositorInsertTGFBR2 wild-type 3'UTR (TGFBR2 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterSV40Available SinceSept. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf106
Plasmid#12794PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf106
ExpressionMammalianAvailable SinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37a, 46]
Plasmid#26569DepositorInsertcalcium channel alpha-1B subunit (Cacna1b Rat)
Tags6XHis and V5ExpressionMammalianMutationDeletion of exons a10, 18a, and 24a. Contains …Available SinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-NmeCas9-NLS-3XHA-NLS
Plasmid#87448PurposeMammalian expression of wild type Nme Cas9DepositorInsertNme Cas9
UseCRISPRTags3XHA, SV40 NLS, and cMyc-like NLSExpressionMammalianPromoterCMVAvailable SinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hS + eGFP
Plasmid#74945PurposeDosage control and tracing of reprogramming episomesDepositorInsertSox2 (SOX2 Human)
ExpressionMammalianAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
KTK_354
Plasmid#180525PurposeKTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf45
Plasmid#12733PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf45
ExpressionMammalianAvailable SinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHRSin-IRES2-EmGFP_HPC-4 Ab heavy-6xHIS
Plasmid#187358PurposeLentiviral expression of HPC-4 antibody heavy chain, and 6xHIS tagDepositorInsertHPC-4 antibody heavy chain, and 6xHIS tag
UseLentiviralPromoterSFFVAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-SV40-RFP
Plasmid#65448PurposeExpression of RFP in mammalian cells. Control vector for FUW-Ephrin-RFP vectorsDepositorInsertSV40-RFP
UseLentiviralExpressionMammalianPromoterUBQAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
LB124 (Bcl-2 from -1287 to -3934)
Plasmid#15240DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterialMutationBcl-2 fragment from -1287 to -3934 with P1Available SinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
TIA1 RRM-YFP-SspBx6
Plasmid#103781Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertTIA1 RRM-YFP-SspBx6
TagsEYFPExpressionMammalianPromoterCMVAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBeast-pAhpC-RBS- LacZ
Plasmid#190060PurposeLacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoterDepositorInsertlacZ
UseSynthetic Biology; Cell-free protein synthesisAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf40
Plasmid#12728PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf40
ExpressionMammalianAvailable SinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Regnase-1 WT
Plasmid#222653PurposeMammalian expression vector to express mouse Regnase-1 WTDepositorAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-RR
Plasmid#83343Purposefor Gateway cloning of promoter elements upstream of a mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsmCherry::Renilla(nls)ExpressionInsectAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
CB6-GFP-TAOK1
Plasmid#197108PurposeExpression of GFP-tagged TAOK1 / PSK2 in mammalian cellsDepositorAvailable SinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo2 mycBioID-Tollip
Plasmid#178054Purposemyc-BioID fused to human Tollip cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.DepositorAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEJS1004-pCSDest2-AcrIIA5-NLS-FLAG
Plasmid#136513PurposeExpresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cellsDepositorInsertcodon-optimized AcrIIA5
TagsFLAG/NLSExpressionMammalianPromoterCMVAvailable SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf107
Plasmid#12795PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf107
ExpressionMammalianAvailable SinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-IFT54
Plasmid#218726PurposeExpresses N-terminally EGFP-tagged IFT54 in mammalian cellsDepositorAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
mChF-Cav1s
Plasmid#171464PurposeExpresses mChF-Cav1s, a FiCE of iCMM, in mammalian cellsDepositorInsertmCherry-FKBP12-Caveolin1(61-178 aa)
ExpressionMammalianPromoterCMVAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-Pax7bait-P2a-GAP43-Cherry-T2a-mERt-Cre-Ert
Plasmid#111153Purposeknock in bait for Axolotl Pax7 locusDepositorInsertGAP43-Cherry-T2a-mERt-Cre-Ert
UseCre/LoxMutationwtAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_Dual_pegRNA
Plasmid#173199PurposeCoselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4 pegRNA with a user-specified pegRNA from two independent U6 promoters. pU6-pegRNA-GG-acceptor-like plasmidDepositorInsertATP1A1 G4 Q118R pegRNA
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
binder/tag-WT-FAK-insertionB
Plasmid#190440PurposeThe tag is SsrA (AANDENY). Tag is inserted after D405 of FAK.DepositorAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
tagSrc(WT)-mCer3 S208F/V224L
Plasmid#179138PurposemCerulean3 has S208F/V224L mutations that promote weak dimerization with YPet to optimize oritentation for FRET. tag is SsrA (AANDENY).DepositorInsertSrc, SsrA
TagsmCerulean3 (S208F/V224L)ExpressionMammalianAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBeast-pAhpC-RBS- sfGFP
Plasmid#190051PurposeGFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoterDepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-VCPIP (OTU, aa 25-561)
Plasmid#61583PurposeExpresses human VCPIP (OTU domain) in E. coli.DepositorInsertVCPIP (VCPIP1 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-24 and aa 562-1222.PromoterT7Available SinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only