59,541 results

1. pcDNA5-hygro-eGFP-HEC1

   Plasmid

   #114027

   Purpose

   Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-CMV-human MYCN-EF1-mRFP

   Plasmid

   #193708

   Purpose

   Lentiviral expression of human MYCN

   Depositor

   Martine Roussel

   Insert

   MYCN (MYCN Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-Ikkb-FLAG WT

   Plasmid

   #23298

   Depositor

   Warner Greene

   Article

   Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

   Insert

   Ikkbeta (IKBKB Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   March 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-AKT-PH-GFP

   Plasmid

   #18836

   Depositor

   Craig Montell

   Article

   Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

   Insert

   AKT-PH domain (AKT1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains amino acids 1-148 (compared to GenBank r…

   Promoter

   CMV

   Available Since

   July 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. NPM1_mNeonGreen_Donor

   Plasmid

   #178093

   Purpose

   HDR donor plasmid to tag NPM1 with mNeonGreen using eSpCas9(1.1)_No_FLAG_NPM1_G6.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   NPM1-mNeonGreen HDR donor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF0095

   Plasmid

   #142145

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZEB2 (ZEB2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Brm WT-Flag pShuttle

   Plasmid

   #122105

   Purpose

   Expression of full length Brm in mammalian cells

   Depositor

   Paul Herring

   Article

   Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

   Insert

   BRM (SMARCA2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-PGK-hESRRB

   Plasmid

   #60434

   Purpose

   Over-expression of human ESRRB

   Depositor

   Austin Smith

   Article

   Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

   Insert

   ESRRB (ESRRB Human)
   

   Expression

   Mammalian

   Available Since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2193

   Plasmid

   #141964

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TFAM (TFAM Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-mKeima-LGALS3

    Plasmid

    #175780

    Purpose

    Lentiviral vector expressing N-terminally tagged mKeima-LGALS3 (Lyso-Keima), for quantitative assessment of lysophagic flux

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2 LRP6-eGFP

    Plasmid

    #180143

    Purpose

    to make movies of LRP6 vesicles

    Depositor

    Edward De Robertis

    Article

    Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

    Insert

    Human LRP6 (LRP6 Human)
    

    Expression

    Mammalian

    Promoter

    SP6

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-SFFV-dCas9-BFP

    Plasmid

    #46910

    Purpose

    Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    dCas9-BFP fusion
    

    Use

    CRISPR and Lentiviral

    Tags

    2xNLS, BFP, and HA

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAd/CMV/DAX1-IRES-nEGFP

    Plasmid

    #29752

    Depositor

    Edward McCabe

    Insert

    dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (NR0B1 Human)
    

    Tags

    EGFP, IRES, and Nuclear localization sequence

    Expression

    Mammalian

    Mutation

    No mutations; Reporter is nuclear localized EGFP;…

    Available Since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-M4-AR

    Plasmid

    #171240

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor (hAR)

    Depositor

    Steven Balk

    Article

    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

    Insert

    3xFlag-tagged human androgen receptor (AR Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. BFP-Rab5CA (Q79L)

    Plasmid

    #205705

    Purpose

    Mammalian expression of BFP-tagged mutant Rab5CA

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    RAB5A (RAB5A Human)
    

    Expression

    Mammalian

    Mutation

    Q79L

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFlag-CMV2-Brd4 del ET

    Plasmid

    #21938

    Depositor

    Eric Verdin

    Article

    Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

    Insert

    BRD4 (BRD4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    aa 1-1362 only, ET domain deleted

    Available Since

    Sept. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

17. NPY5R-Tango

    Plasmid

    #66456

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    NPY5R (NPY5R Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1

    Plasmid

    #214995

    Purpose

    Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1_gRNA

    Plasmid

    #196139

    Purpose

    gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    AAVS1 gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV5B-Flag-Smad3

    Plasmid

    #11742

    Depositor

    Jeff Wrana

    Article

    Labbe et al Mol Cell. 1998 Jul . 2(1):109-20.

    Insert

    SMAD3 (SMAD3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 7, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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