We narrowed to 21,643 results for: Arc

1. 1255_pAAV-U6-SA-BbsI-MluI-gRNA-CB-SACas9-HA-OLLAS-spA

   Plasmid

   #109320

   Purpose

   Plasmid for AAV SaCas9 Mammalian Expression with a BbsI cloning site for a gRNA

   Depositor

   William Lagor

   Article

   Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. [pSK467] pLJC5 HA-Depdc5

   Plasmid

   #109343

   Purpose

   Lenti-viral expression of HA-tagged wildtype Depdc5

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Rescue Septin 11 mRNA

   Plasmid

   #122022

   Purpose

   Rescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.

   Depositor

   Angel de Blas

   Article

   Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

   Insert

   SEPT11 (Sept11 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Silent mutations are in 5 consecutive codons (AUU…

   Promoter

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTS2303-Tier1-CoTC-PPGK-NLuc_TB

   Plasmid

   #169548

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   CoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Ai34

   Plasmid

   #34881

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

   Insert

   CAG-Floxed Synaptophysin-tdTomato
   

   Use

   Mouse Targeting

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   CAG

   Available since

   March 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJT130_GalL_nCDA1-NGBE3

   Plasmid

   #145094

   Purpose

   Expressing base editor nCDA1-NGBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   nCDA1-NGBE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

   Promoter

   GalL

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDIV303

   Plasmid

   #204978

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and amdS selection marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   amdS
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   two silent mutations (nt177T>C, nt879C>T)

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 6598-HisSUMO1GG

   Plasmid

   #13376

   Depositor

   Anne Dejean

   Article

   Seeler et al Mol Cell Biol. 2001 May . 21(10):3314-24

   Insert

   SUMO1 (SUMO1 Human)
   

   Use

   T7 in vitro translation

   Tags

   His

   Expression

   Mammalian

   Mutation

   mature form ending with GG

   Promoter

   Available since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CMV-mito-CAR-GECO1

   Plasmid

   #46022

   Purpose

   Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Neurosci. 2013 Mar 19.

   Insert

   CAR-GECO1
   

   Use

   Tags

   a duplex of the mitochondrial targeting signal of…

   Expression

   Mammalian

   Mutation

   R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P

   Promoter

   CMV

   Available since

   July 23, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-hGFAP-Pink Flamindo-WPRE-SV40

    Plasmid

    #178790

    Purpose

    AAV vector to express the red cAMP indicator in astrocytes

    Depositor

    Tetsuya Kitaguchi

    Article

    Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

    Insert

    Pink Flamindo
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hGFAP

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMSCV-FlagMLL2-pL-ENL(5613)

    Plasmid

    #20874

    Depositor

    Robert Slany

    Article

    Bach et al Oncogene. 2009 Feb 12. 28(6):815-23.

    Insert

    MLL2 (Kmt2d, MLLT1 Human, Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pRK5 DN-Fyn

    Plasmid

    #16033

    Depositor

    Filippo Giancotti

    Article

    Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

    Insert

    c-Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Dominant negative: K299M

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pEBB COMMD3 Flag

    Plasmid

    #74895

    Purpose

    Express COMMD3 in mammalian cells

    Depositor

    Colin Duckett, Ezra Burstein

    Article

    Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

    Insert

    COMMD3 (COMMD3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha promoter

    Available since

    June 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSF-G1(4CNW)RS

    Plasmid

    #222871

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Cyano-L-Tryptophan (4-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLS.419

    Plasmid

    #187208

    Purpose

    Expresses wild-type retron Eco4 ncRNA, and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco4 ncRNA
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7/Lac

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-mNeptune2

    Plasmid

    #51309

    Purpose

    Expresses far-red fluorescent protein mNeptune2 in mammalian cells

    Depositor

    Michael Lin

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    mNeptune2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21

    Plasmid

    #138295

    Purpose

    gRNA for CRISPR/Cas9 knockout of human TRIM21

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    gRNA TRIM21 (TRIM21 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCFD5-U6-3-t-attP40

    Plasmid

    #133561

    Purpose

    gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    attP40 region guide RNAs
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMW96

    Plasmid

    #178066

    Purpose

    Expression of His6-SUMO-TEV-GFP-PreScission-γ-TuNA in E. coli

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al Cell. 2020 Jan 9;180(1):165-175.e16. doi

    Insert

    CDK5RAP2 (CDK5RAP2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-C1-BBS5

    Plasmid

    #218714

    Purpose

    Expresses N-terminally EGFP-tagged BBS5 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    BBS5 (BBS5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. His-MBP-NRBF2

    Plasmid

    #99330

    Purpose

    Mammalian expression of NRBF2, which associates with the PI3KC3-C1 complex

    Depositor

    James Hurley

    Article

    Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

    Insert

    Nuclear receptor-binding factor 2 (NRBF2 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-ITR-PytR-mCherry

    Plasmid

    #204868

    Purpose

    Expresses pyrrolysyl tRNA and a wild-type mCherry reporter; can be packaged into AAV

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

    Insert

    M. mazei pyrrolysyl tRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    U25C

    Promoter

    U6

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PB-empty-DSE-TetO-8kb

    Plasmid

    #213022

    Purpose

    piggyBac donor vector containing an 8-kb TetO operator array for random integration using PBase transposase

    Depositor

    Luca Giorgetti

    Article

    Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

    Insert

    TetO
    

    Use

    Mouse Targeting; Piggybase integration (transposo…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. eGFP-pk-S65A

    Plasmid

    #115619

    Purpose

    Expresses human Parkin S65A in mammalian cells

    Depositor

    Edward Fon

    Article

    Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S65A

    Promoter

    CMV

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDM002-sgRNA-lenti-ZeoR

    Plasmid

    #200060

    Purpose

    Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    Bleomycin resistance, mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDule-Mb haloTyrRS C6

    Plasmid

    #160377

    Purpose

    C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.

    Depositor

    Ryan Mehl

    Article

    Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

    Insert

    C6 HaloTyrosine tRNA synthatase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    L270S, Y271L, N311G, C313T

    Promoter

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTracer_Cr-TRP1

    Plasmid

    #64881

    Purpose

    Expresses CrTRP1 in mammalian cells

    Depositor

    Sebastián Brauchi

    Article

    Arias-Darraz et al Plant Cell. 2015 Jan;27(1):177-88. doi:

    Insert

    Transient receptor potential 1
    

    Use

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pLux01

    Plasmid

    #78281

    Purpose

    mRFP1 expressed behind an AHL-inducible construct

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    AHL inducible mRFP1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pB-Ric8b-Integration

    Plasmid

    #129457

    Purpose

    piggyBac transposon vector that Inducibly (Tet-On) expresses Ric8b

    Depositor

    Sriram Kosuri

    Article

    Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

    Insert

    Ric8b (Ric8b Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE (Tet-On)

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

    Plasmid

    #98974

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 3 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBp-FGFR3c-WT

    Plasmid

    #45711

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR3 IIIc (FGFR3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. [pSK468] pLJC5 Flag-Depdc5

    Plasmid

    #109337

    Purpose

    Lenti-viral expression of Flag-tagged wildtype Depdc5

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEVOL-AcKRS-CloDF

    Plasmid

    #127415

    Purpose

    Mutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophage

    Depositor

    Wenshe Liu

    Article

    Wang et al Angew Chem Int Ed Engl. 2019 Oct 28;58(4

    Insert

    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCEP4-HA-Flag-natT1R2

    Plasmid

    #113945

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    residues 22-839

    Promoter

    CMV

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. aav-tnt-dnmrtfa-gfp

    Plasmid

    #165035

    Purpose

    AAV-based delivery of dnMRTFA-GFP into cardiomyocytes in vivo

    Depositor

    William Pu

    Article

    Guo et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

    Insert

    dnMRTFA
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mutation

    deletion of 100 N-terminal amino acids

    Promoter

    cTNT

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCW57.1_V5_mNeonGreen_FLAG_C

    Plasmid

    #218073

    Purpose

    RESOLUTE cloning plasmid

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FLAG-mNeonGreen-V5

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLenti Dest PuroR R1-R2

    Plasmid

    #84575

    Purpose

    Gateway destination vector expressing Puromycin resistance under a PGK promoter

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Type

    Empty backbone

    Use

    Lentiviral; Gateway

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGEX-4T-1 UBXN2A

    Plasmid

    #113505

    Purpose

    Bacterial expression of GST-tagged UBXN2A

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    UBXN2A (UBXN2A Human)
    

    Use

    Tags

    GST-Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. LacI-pLacI-pIII pm

    Plasmid

    #218100

    Purpose

    Phagemid that expresses LacI, and pIII under the Lac promoter

    Depositor

    Kevin Esvelt

    Insert

    pIII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAS_FLAG-G1 PylRS

    Plasmid

    #154763

    Purpose

    plasmid with G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pHalo-MyosinIIB-C25

    Plasmid

    #128574

    Purpose

    Studies of cellular contractility.

    Depositor

    Dylan Burnette

    Article

    Fenix et al Elife. 2018 Dec 12;7. pii: 42144. doi: 1

    Insert

    Myosin IIB (MYH10 Human)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Flag Intersectin Long (human)

    Plasmid

    #47393

    Depositor

    Peter McPherson

    Article

    Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

    Insert

    intersectin long (ITSN1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

43. pRSF-G1(6CNW)RS

    Plasmid

    #222873

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcDNA3_ERBB2-V5

    Plasmid

    #214612

    Purpose

    ERBB2 receptor for ERBB2/ERBB3 heterodimer split TEV recuitment assay

    Depositor

    Michael Wehr

    Article

    Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. JF001A

    Plasmid

    #53116

    Purpose

    expresses alpha hemolysin under the control of theophylline riboswitch

    Depositor

    Sheref Mansy

    Article

    Lentini et al Nat Commun. 2014 May 30;5:4012. doi: 10.

    Insert

    theophylline riboswitch and alpha hemolysin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    May 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. J23100-mRFP1-331Bb

    Plasmid

    #78271

    Purpose

    mRFP1 behind constitutive promoter J23100 in pSEVA331Bb backbone

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    J23100-mRFP1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pBAD02_CBASS_Escherichia_coli_CVM_N37067PS

    Plasmid

    #224403

    Purpose

    For expression of the type I CBASS system encoded by Escherichia coli CVM N37067PS

    Depositor

    Philip Kranzusch

    Article

    Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

    Insert

    EcCdnF_AGS-C_SLATT6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBAD02_CBASS_Escherichia_coli_BIDMC103

    Plasmid

    #224402

    Purpose

    For expression of the type I CBASS system encoded by Escherichia coli BIDMC103

    Depositor

    Philip Kranzusch

    Article

    Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

    Insert

    EcCdnF_AGS-C_SLATT6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pBAD02_CBASS_Escherichia_coli_K71-77

    Plasmid

    #224398

    Purpose

    For expression of the type I CBASS system encoded by Escherichia coli K71-77

    Depositor

    Philip Kranzusch

    Article

    Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

    Insert

    Phospholipase_EcCdnE_DisA-like
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pBAD02_CBASS_Escherichia_coli_RU1_BHI6

    Plasmid

    #224397

    Purpose

    For expression of the type I CBASS system encoded by Escherichia coli RU1 BHI6

    Depositor

    Philip Kranzusch

    Article

    Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

    Insert

    Cap15_EcCdnE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge