We narrowed to 25,908 results for: Nes

Showing: 11851 - 11900 of 25908 results

Results per page:

Sort results by:

pLenti_CMV:Flag-GpNLuc_SV40:PuroR

Plasmid

#183037

Purpose

Expresses the FLAG-tagged GpNLuc (Green LumiFluor) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

GpNLuc LumiFluor

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p1.2-Neo

Plasmid

#162739

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CHO Elongation factor 1A

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMV:Flag-OgNLuc_SV40:PuroR

Plasmid

#183038

Purpose

Expresses the FLAG-tagged OgNLuc (Orange LumiFluor) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

OgNLuc LumiFluor

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pV1435

Plasmid

#111439

Purpose

Solo vector pV1382 + sgCgADE2

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgCgADE2

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCORE-Hp53

Plasmid

#72242

Purpose

Contains the CORE cassette with hyg and human p53 gene markers

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

p53 (TP53 Human)
hyg

Expression

Bacterial

Mutation

V122A mutation

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBluescript SK +/- mTRF1

Plasmid

#12303

Depositor

Titia de Lange

Article

Broccoli et al Hum Mol Genet. 1997 Jan . 6(1):69-76.

Insert

mTRF1 (Terf1 Mouse)

Expression

Bacterial

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only
gRNA-mCtnnb1

Plasmid

#84542

Purpose

mCtnnb1 gRNA expressing vector

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

mCtnnb1 gRNA

Use

CRISPR

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mFzd6-1D4

Plasmid

#42268

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 6 (Fzd6 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pTG131

Plasmid

#198640

Purpose

Thermosensitive pSC101-based plasmid expressing a sgRNA under the control of the J23119 promoter and Cas9 under the control of the DAPG-inducible PhlF promoter

Depositor

David Bikard

Article

Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

Insert

Cas9

Expression

Bacterial

Promoter

pPhlF

Available Since

June 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pML107-kanR468

Plasmid

#162041

Purpose

Expresses Cas9 and contains kanR468 guide which targets the kanR gene

Depositor

Jolanda Van Leeuwen

Article

Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

Insert

kanR468 guide

Use

CRISPR

Expression

Yeast

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTH

Plasmid

#56659

Purpose

Dual-expression vector for P. tricornutum

Depositor

Philip Weyman

Article

Weyman et al Plant Biotechnol J. 2014 Oct 10. doi: 10

Insert

KanR

Use

Synthetic Biology; Diatom expression

Available Since

Oct. 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT344_pattB-pCA-GFP-pA-FRT5

Plasmid

#52550

Purpose

for Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing mice

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-beta-hRLuc-V5-6xbsMS2-MALAT_X

Plasmid

#147924

Purpose

Mammalian Expression of 6xbsMS2-Malat

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

6xbsMS2-Malat (Malat1 Mouse)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pIS1 Cyclin D2 long UTR

Plasmid

#21645

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 long UTR (CCND2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FBL6 delta6

Plasmid

#19925

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

FBL6 delta 6 (FBXL6 Human)

Expression

Mammalian

Mutation

delta 6

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only
pJG380

Plasmid

#91188

Purpose

BeYDV replicon T-DNA for gene targeting in tobacco leaves, nuclease (AtCas9+gNt_R2+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9+gNt_R2+donor

Use

CRISPR

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR1A_no_ccDB_mUty

Plasmid

#158570

Purpose

V5-tagged sequence of mUty cloned into entry vector for recombination

Depositor

Christian Deschepper

Article

Deschepper et al Sci Rep. 2020 Sep 10;10(1):14900. doi: 1

Insert

Mus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)

Use

Lentiviral

Tags

V5

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTrichoGate-4

Plasmid

#160704

Purpose

Trichoderma optimised Synthetic mCherry bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

optimised synthetic mCherry

Use

Synthetic Biology

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJP118

Plasmid

#176494

Purpose

Expresses Lifeact-mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter

Depositor

Duojia Pan

Article

Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

Insert

Lifeact peptide

Use

Capsaspora owczarzaki expression

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-Merlin-Flag-HA

Plasmid

#27103

Depositor

Filippo Giancotti

Article

Li et al Cell. 2010 Feb 19. 140(4):477-90.

Insert

Merlin (Nf2 Mouse)

Tags

Flag and HA

Expression

Mammalian

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pEF1α-mouse-retroCHMP3-HA

Plasmid

#154178

Purpose

expresses mouse retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
HIR Y1158F

Plasmid

#24051

Depositor

Frederick Stanley

Article

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Insert

Insulin Receptor (INSR Human)

Expression

Mammalian

Mutation

Y1158F

Available Since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only
HIR Y1162F

Plasmid

#24052

Depositor

Frederick Stanley

Article

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Insert

Insulin Receptor (INSR Human)

Expression

Mammalian

Mutation

Y1162F

Available Since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only
HIR Y1163F

Plasmid

#24053

Depositor

Frederick Stanley

Article

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Insert

Insulin Receptor (INSR Human)

Expression

Mammalian

Mutation

Y1163F

Available Since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only
pT3TS-nCas9n-A71

Plasmid

#125644

Purpose

micro-injection of poly-adenylated nCas9n RNA

Depositor

Jason Berman

Article

Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

Insert

nCas9n

Use

Rna in vitro transcription vector

Promoter

T3

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-Arl13b1

Plasmid

#40870

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Gateway Cloning

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3 -5'cMyc-SMCl- S957A

Plasmid

#32368

Depositor

Michael Kastan

Article

Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

Insert

SMC1A (SMC1A Human)

Tags

c-Myc

Expression

Mammalian

Mutation

S957A

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pc-GAKIN-Ha

Plasmid

#188246

Purpose

Express GAKIN in mammalian cells with Ha tag

Depositor

Joel Pomerantz

Article

Lamason et al Mol Cell. 2010 Dec 10;40(5):798-809. doi

Insert

GAKIN (KIF13B Human)

Tags

Ha

Expression

Mammalian

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 5' cMyc- SMCI-S966A

Plasmid

#32371

Depositor

Michael Kastan

Article

Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

Insert

SMC1A (SMC1A Human)

Tags

c-Myc

Expression

Mammalian

Mutation

S996A

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pLV-H1-SGIPZ_NF1 sh1miR

Plasmid

#119206

Purpose

knockdown of NF1

Depositor

Helen Morrison

Article

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

sh1miR targeting NF1 (NF1 Mouse, Human, Rat)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mFzd10-1D4

Plasmid

#42272

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 10 (Fzd10 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pET21(+)-His6-Ubc13

Plasmid

#212715

Purpose

Bacterial expression of His6-Ubc13

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Ubc13

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pH6HTC_CCDC59-Halo

Plasmid

#175327

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

CCDC59 (CCDC59 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR_eGFP_369

Plasmid

#111823

Purpose

Knockout eGFP

Depositor

Steve Shih

Article

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Insert

gRNA targeting eGFP 349-369

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-Pidd1(T788D)

Plasmid

#60530

Purpose

Retroviral expression of Pidd1 T788D phosphorylation mutant

Depositor

Trudy Oliver

Article

Terry et al Cell Death Differ. 2014 Oct 10. doi: 10.

Insert

Pidd1 (PIDD1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Threonine 788 to Aspartic acid*

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only
pRU52

Plasmid

#167678

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUB4-2::spCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PGL3-MK17

Plasmid

#21486

Depositor

Shoumo Bhattacharya

Article

Leung et al Genomics. 1999 Nov 1. 61(3):307-13.

Insert

Cited2 Promoter (CITED2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 23, 2009

Availability

Academic Institutions and Nonprofits only
pCaUra3-CUbo-AID*-GFP

Plasmid

#212775

Purpose

C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with CaUra3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRTP801/TA-ODD

Plasmid

#206212

Purpose

An expression vector of hypoxia-responsive transactivator under control of RTP801 promoter

Depositor

Masamichi Kamihira

Article

Masumoto et al J Biosci Bioeng. 2021 Oct;132(4):399-407

Insert

tetracycline-responsive transactivator fused with oxygen-dependent degradation domain

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_A0908

Plasmid

#91027

Purpose

Module A, Promoter: EC1.2, Gene: TREX2-P2A-AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TREX2-P2A-AtCas9

Use

CRISPR

Promoter

EC1.2

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEMT-PT2A-Tdtomato-iRFP670-GFP

Plasmid

#111815

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

Tdtomato
iRFP670
GFP

Use

Cloning intermediate

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
E12 2/5-pCS2

Plasmid

#20913

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

E12 2/5 (TCF3 Human)

Expression

Mammalian

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3

Plasmid

#21800

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

PSMD14 (Psmd14 Mouse)

Tags

GST and TEV

Expression

Mammalian

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pSR-puro-Exo84

Plasmid

#31116

Depositor

Christopher Counter

Article

Issaq et al Mol Cancer Res. 2010 Feb . 8(2):223-31.

Insert

Exo84 shRNA (EXOC8 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only
pME-puro-CLIP-FLAG-CD59

Plasmid

#50375

Purpose

Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

CLIP

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-nlsGal80(opt)

Plasmid

#51618

Purpose

vertebrate expression of codon-optimized Gal80 with N-terminal NLS and no stop

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

Gal80(opt) (GAL80 Budding Yeast)

Use

Vertebrate expression

Tags

NLS

Mutation

codon optimized

Available Since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMB-MTUBr

Plasmid

#61197

Purpose

Fluorescent reporter for microtubules mCherry-MAP4-MBD expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MAP-MBD

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pETCON-TNF

Plasmid

#67748

Purpose

Tumor Necrosis Factor Alpha Yeast Display Plasmid

Depositor

Timothy Whitehead

Article

Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

Insert

TNF-Alpha

Expression

Bacterial and Yeast

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p663-UBC-miniTurbo-V5-CSNK1G2_IDG-K

Plasmid

#214459

Purpose

Gateway destination clone of CSNK1G2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CSNK1G2 (CSNK1G2 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA-LEU2-I-SceI

Plasmid

#67595

Purpose

I-SceI expression regulated by tetracycline inducible system in S. pombe

Depositor

Kurt Runge

Article

Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

Inserts

nmt terminator
I-SceI
TetR

Use

Gene targeting in yeast

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 11851 - 11900 of 25908 results

Results per page:

Sort results by:

More Filters

We narrowed to 25,908 results for: Nes