Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 52,941 results for: plasmid

Showing: 11851 - 11900 of 52941 results

  1. pCMV5 HA-TGFBRII

    Plasmid
    #19158
    Depositor
    Joan Massague
    Insert
    Transforming growth factor, beta receptor II (TGFBR2 Human)
    Use
    Tags
    HA (YDVPDYASL)
    Expression
    Mammalian
    Mutation
    PCR based mutagenesis
    Promoter
    Available since
    Oct. 1, 2008
    Availability
    Academic Institutions and Nonprofits only
    19158-plasmid-map-sequence-id-364716

    Enlarge

  2. pKG123

    Plasmid
    #63074
    Purpose
    Retroviral expression of mCherry-TEV-S-tag CENP-C for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-C (CENPC Human)
    Use
    Retroviral
    Tags
    TEV-S and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    63074-plasmid-map-sequence-id-135907

    Enlarge

  3. pFLAG-CMV-hErk1(K71R)

    Plasmid
    #49329
    Purpose
    Expresses kinase deficient FLAG-tagged human Erk1
    Depositor
    Melanie Cobb
    Insert
    Erk1 (MAPK3 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    K71R
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. hC73S-pEGFP-N1

    Plasmid
    #111536
    Purpose
    Expresses human KCNB1 C73S in mammalian cells for imaging
    Depositor
    Federico Sesti
    Insert
    human C73S-Kv2.1 (KCNB1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    C73S
    Promoter
    CMV
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111536-plasmid-map-sequence-id-211633

    Enlarge

  6. IDG_CLCC1_OE_1

    Plasmid
    #161672
    Purpose
    Overexpresses CLCC1
    Depositor
    Mike McManus
    Insert
    CLCC1 (CLCC1 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    161672-plasmid-map-sequence-id-319664

    Enlarge

  7. Pet28a_Synthetic_Human_H2AZ.1

    Plasmid
    #42629
    Depositor
    Joe Landry
    Insert
    Human H2AZ.1 (H2AZ1 Codon Optimized, Human, Synthetic)
    Use
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Codon Optimized
    Promoter
    T7
    Available since
    March 21, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42629-plasmid-map-sequence-id-434040

    Enlarge

  8. MSCV-FoxP3-R337Q-IRES-Thy1.1

    Plasmid
    #229617
    Purpose
    Mammalian expression of full length mouse FoxP3 (R337Q)
    Depositor
    Sun Hur
    Insert
    full length FoxP3, carrying single point mutation R337Q (Foxp3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R337Q
    Promoter
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229617-plasmid-map-sequence-id-457335

    Enlarge

  9. MSCV-FoxP3-A372S-IRES-Thy1.1

    Plasmid
    #229618
    Purpose
    Mammalian expression of full length mouse FoxP3 (A372S)
    Depositor
    Sun Hur
    Insert
    full length FoxP3, carrying single point mutation A372S (Foxp3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    A372S
    Promoter
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229618-plasmid-map-sequence-id-457333

    Enlarge

  10. pMSCV_puro_41584

    Plasmid
    #111630
    Purpose
    Complementation of Dicer_KO mESCs with wild type human DICER isoform
    Depositor
    Constance Ciaudo
    Insert
    FLAG-hsDicer (DICER1 Human)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    111630-plasmid-map-sequence-id-217596

    Enlarge

  11. pcDNA3.3_SARS2_BA.2.86

    Plasmid
    #208441
    Purpose
    expressing SARS-CoV-2 BA.2.86 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (BA.2.86) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    see GISAID sequence
    Promoter
    CMV
    Available since
    Oct. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    208441-plasmid-map-sequence-id-410934

    Enlarge

  12. pcDNA5-FRT-GFP-MYC

    Plasmid
    #185375
    Purpose
    For mammalian expression of human GFP-MYC
    Depositor
    Ayaz Najafov
    Insert
    MYC (MYC Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    185375-plasmid-map-sequence-id-363433

    Enlarge

  13. CUP1-∆ssCPY*-mGFP in pRS315

    Plasmid
    #221158
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mGFP
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    221158-plasmid-map-sequence-id-450962

    Enlarge

  14. CUP1-∆ssCPY*-mScarletI in pRS315

    Plasmid
    #221159
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mScarletI
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221159-plasmid-map-sequence-id-450960

    Enlarge

  15. IDG_CHRNA2_OE_1

    Plasmid
    #161648
    Purpose
    Overexpresses CHRNA2
    Depositor
    Mike McManus
    Insert
    CHRNA2 (CHRNA2 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    161648-plasmid-map-sequence-id-319595

    Enlarge

  16. pMX-Flag-GFP_HOMER2

    Plasmid
    #227953
    Purpose
    Co-localisation experiment
    Depositor
    Alessandro Ori
    Insert
    HOMER2 (HOMER2 Human)
    Use
    Lentiviral
    Tags
    3xFLAG-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    227953-plasmid-map-sequence-id-455411

    Enlarge

  17. pLKO.1 LARP7-FLAG 7SK

    Plasmid
    #200733
    Purpose
    Lentiviral expression of Flag-Tagged human LARP7 and the snRNA 7SK
    Depositor
    Claudio Alarcón
    Insert
    LARP7 and 7SK (LARP7 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    200733-plasmid-map-sequence-id-401803

    Enlarge

  18. IDG_CACNA2D2_OE_1

    Plasmid
    #161616
    Purpose
    Overexpresses CACNA2D2
    Depositor
    Mike McManus
    Insert
    CACNA2D2 (CACNA2D2 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    161616-plasmid-map-sequence-id-318722

    Enlarge

  19. pET28a-mOct4 Full length(FL)

    Plasmid
    #206394
    Purpose
    Expresses Oct4 with a 6xHis tag for protein purification
    Depositor
    Ralf Jauch
    Insert
    Oct4 (Pou5f1 Mouse)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    206394-plasmid-map-sequence-id-409984

    Enlarge

  20. MEK1-E102-I103del-V5

    Plasmid
    #217301
    Purpose
    Lentivirus vector for expression of MEK1 E102-I103deletion
    Depositor
    Channing Der
    Insert
    MAP2K1 (MAP2K1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mutation
    E102-I103deletion
    Promoter
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    217301-plasmid-map-sequence-id-431010

    Enlarge

  21. pCMV-ABE-P48R-UGI

    Plasmid
    #161817
    Purpose
    Expresses ABE-P48R-UGI in mammalian cells
    Depositor
    Sangsu Bae
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Promoter
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    161817-plasmid-map-sequence-id-320651

    Enlarge

  22. pUAST_mLExA-VP16-NFATc1 (C-del)

    Plasmid
    #35023
    Depositor
    Jing-W Wang
    Insert
    NFATc1 (NFATC1 Human)
    Use
    Tags
    VP16 and mLexA
    Expression
    Insect
    Mutation
    deletion of amino acids 590-716
    Promoter
    UAS
    Available since
    March 8, 2012
    Availability
    Academic Institutions and Nonprofits only
    35023-plasmid-map-sequence-id-183825

    Enlarge

  23. Grx1-roGFP2-Dcp1b

    Plasmid
    #236547
    Purpose
    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in processing bodies.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Grx1-roGFP2-Dcp1b (DCP1B Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236547-plasmid-map-sequence-id-469940

    Enlarge

  24. Grx1-roGFP2-Coilin

    Plasmid
    #236546
    Purpose
    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in Cajal bodies.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Grx1-roGFP2-Coilin (COIL Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236546-plasmid-map-sequence-id-469938

    Enlarge

  25. SOX9-T2A-NLS-tdTomato-PGK-Puro

    Plasmid
    #89993
    Purpose
    Donor template for generation of SOX9-ntdTomato reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    none
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    89993-plasmid-map-sequence-id-180698

    Enlarge

  26. Flag-neo-PP1-Gamma

    Plasmid
    #171270
    Purpose
    Mammalian expression of 3x-Flag-tagged human PP1 gamma catalytic subunit isoform 1
    Depositor
    Steven Balk
    Insert
    3x-Flag-tagged human PP1 gamma catalytic subunit isoform 1 (PPP1CC Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171270-plasmid-map-sequence-id-335789

    Enlarge

  27. pENTR1A-Huwe1 C4341S

    Plasmid
    #37432
    Depositor
    Jean Cook
    Insert
    Huwe1 (HUWE1 Human)
    Use
    Gateway entry
    Tags
    Expression
    Mutation
    C4341S
    Promoter
    Available since
    Sept. 25, 2012
    Availability
    Academic Institutions and Nonprofits only
    37432-plasmid-map-sequence-id-146007

    Enlarge

  28. pcDNA3.1 AKT3 HA (CT-gfp)

    Plasmid
    #27293
    Depositor
    Morris Birnbaum
    Insert
    protein kinase B gamma (Akt3 Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Y473F
    Promoter
    Available since
    April 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  29. pcDNA3.1-hUCP4-NE

    Plasmid
    #102362
    Purpose
    Overexpression of solute carrier family 25 member 27 in mammalian cells
    Depositor
    Shu Leong Ho
    Insert
    solute carrier family 25 member 27 (SLC25A27 Human)
    Use
    Tags
    NE
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    102362-plasmid-map-sequence-id-200644

    Enlarge

  30. pJ609 Flag-Sumo1

    Plasmid
    #49046
    Purpose
    Mammalian expression of N-terminally tagged Sumo1
    Depositor
    Lianna Swanson
    Insert
    SUMO1 (SUMO1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    49046-plasmid-map-sequence-id-72029

    Enlarge

  32. pLenti-TRE-PtenWT-Blast

    Plasmid
    #135674
    Purpose
    Dox-inducible expression (TRE promoter) of wildtype Pten cDNA, Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    Pten (Pten Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135674-plasmid-map-sequence-id-327578

    Enlarge

  34. p5E-elavl3

    Plasmid
    #72640
    Purpose
    Gateway p5E 5'entry clone with elavl3 (HuC) enhancer/promoter for pan-neuronal expression in zebrafish
    Depositor
    Joshua Bonkowsky
    Insert
    elavl3 enhancer (elavl3 Zebrafish)
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72640-plasmid-map-sequence-id-141850

    Enlarge

  35. pcDNA3.1-MS2-HA-HsDIS3_Z

    Plasmid
    #148111
    Purpose
    Mammalian Expression of HsDIS3
    Depositor
    Elisa Izaurralde
    Insert
    HsDIS3 (DIS3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L760F compared to the sequence given by isoform 2…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148111-plasmid-map-sequence-id-407051

    Enlarge

  36. IDG_CACNB1_OE_1

    Plasmid
    #161660
    Purpose
    Overexpresses CACNB1
    Depositor
    Mike McManus
    Insert
    CACNB1 (CACNB1 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    161660-plasmid-map-sequence-id-319641

    Enlarge

  37. pET-HIS-Sangamo

    Plasmid
    #40786
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    N Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    T7
    Available since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    40786-plasmid-map-sequence-id-68901

    Enlarge

  38. R26-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

    Plasmid
    #180152
    Purpose
    tdTomato cre reporter with Rosa26 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180152-plasmid-map-sequence-id-355317

    Enlarge

  40. pET-Sangamo-HIS

    Plasmid
    #40787
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    C Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus,Codon Optimized FokI Endonuclease
    Promoter
    T7
    Available since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    40787-plasmid-map-sequence-id-68902

    Enlarge

  41. pcDNA3.1-HA_human_BAP1

    Plasmid
    #158109
    Purpose
    Expresses tagged BAP1, neomycin selection
    Depositor
    Emanuela Felley-Bosco
    Insert
    BAP1 (BAP1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    158109-plasmid-map-sequence-id-309425

    Enlarge

  42. pcDNA3.1 CARMA1

    Plasmid
    #44431
    Depositor
    Xin Lin
    Insert
    CARMA1 (CARD11 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    See depositor comments below.
    Promoter
    Available since
    Oct. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    44431-plasmid-map-sequence-id-263620

    Enlarge

  43. XLone-Puro SPI1 P2A mCherry

    Plasmid
    #140029
    Purpose
    Tunable and temporal expression control of SPI1 and mCherry
    Depositor
    Xiaoping Bao
    Insert
    SPI1 (SPI1 Human)
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140029-plasmid-map-sequence-id-275844

    Enlarge

  44. p667-UBC-GFP-V5_IDG-K

    Plasmid
    #135242
    Purpose
    Gateway destination clone of GFP (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    GFP-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    135242-plasmid-map-sequence-id-270199

    Enlarge

  45. IDG_CACNG1_OE_1

    Plasmid
    #161650
    Purpose
    Overexpresses CACNG1
    Depositor
    Mike McManus
    Insert
    CACNG1 (CACNG1 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    161650-plasmid-map-sequence-id-319346

    Enlarge

  46. RCAS(A) mFgfr1 DN (CT#984)

    Plasmid
    #14006
    Depositor
    Cliff Tabin
    Insert
    Fgfr1 (Fgfr1 Mouse)
    Use
    Retroviral; Avian expression
    Tags
    Expression
    Mutation
    Dominant negative
    Promoter
    Available since
    Feb. 16, 2007
    Availability
    Academic Institutions and Nonprofits only
    14006-plasmid-map-sequence-id-465063

    Enlarge

  47. hGli1 6x his

    Plasmid
    #84923
    Purpose
    mammalian expression of Gli1
    Depositor
    Martin Fernandez-Zapico
    Insert
    Gli1 (GLI1 Human)
    Use
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  48. XLone-BSD SPI1 P2A mCherry

    Plasmid
    #140031
    Purpose
    Tunable and temporal expression control of SPI1 and mCherry
    Depositor
    Xiaoping Bao
    Insert
    SPI1 (SPI1 Human)
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140031-plasmid-map-sequence-id-275874

    Enlarge

  49. pIRES-H3.3-G34R-FLAG-BFP

    Plasmid
    #206140
    Purpose
    Expression of a FLAG tagged human histone mutant H3.3-G34R with BFP
    Depositor
    Maria Castro
    Insert
    H3.3 (H3-3A Human)
    Use
    Tags
    FLAG-eBFP
    Expression
    Mammalian
    Mutation
    G34R
    Promoter
    Available since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    206140-plasmid-map-sequence-id-405579

    Enlarge

  50. CACNA2D1-TwinStrepII

    Plasmid
    #83192
    Purpose
    Expression of tagged protein in mammalian cells
    Depositor
    Peter Barr-Gillespie
    Insert
    CACNA2D1 (Cacna2d1 Rat)
    Use
    Tags
    Twin Strep Tag II
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    83192-plasmid-map-sequence-id-163487

    Enlarge

Showing: 11851 - 11900 of 52941 results