178,697 results
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Plasmid#1735PurposeThis retroviral vector expresses lacZ and has an IRES-NEO downstreamDepositorInsertLacZ-IRES-Neo
UseRetroviralAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCruzHA SIRT1 G261A
Plasmid#10963DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSIRT1 (Sirt1 Mouse)
TagsHAExpressionMammalianMutationG261AAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
kiCAP-AAV-MyoAAV2A
Plasmid#224440PurposeRep/Cap plasmid for the production of MyoAAV 2A, a muscle-tropic AAV capsid in mice.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationRGDQTTL insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Nuclear PancACe
Plasmid#215706PurposeExpresses acetyl-CoA biosensor in the nucleusDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and NLSExpressionMammalianPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
gag-MCP-pol
Plasmid#211370PurposePackaging plasmid for v3 PE-eVLP productionDepositorInsertMMLVgag-MCP-pol
ExpressionMammalianAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8.20
Plasmid#194702PurposeExpresses N-terminal MBP fused TadA8.20 in bacteriaDepositorInsertTadA8.20
Tags6xHis and MBPExpressionBacterialMutationmutations in TadA are described in the paperPromoterT7 promoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PiggyBac dCas9-VPR
Plasmid#191269PurposepiggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsVPR-dCas9-HA-tagBFP
rtTA3-IRES-PURO
UseCRISPRTagsHA and tagBFPExpressionMammalianAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ER-GCaMP6f
Plasmid#182548PurposeExpresses GCaMP6f on the cytosolic side of the ER (P-450site)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertER localized P450
ExpressionBacterial and MammalianAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-nEF-Con/Fon DREADD Gi-mCherry
Plasmid#177672PurposeExpresses DREADD hM4D (Gi) in Cre and Flp expressing cellsDepositorHas serviceAAV8InsertDREADD Gi-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianPromoternEFAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCV-dCasRx-ALKBH5-PURO
Plasmid#175582PurposeExpresses dCasRx-ALKBH5 in mammalian cellDepositorInsertdCasRx-ALKBH5
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationRfxCas13d R239A/H244A/R858A/H863APromoterpMSCVAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEG BacMam C term 3C eGFP StrepII
Plasmid#160686PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneTags3C eGFP StrepIIExpressionMammalianPromoterCMVAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TBG-ER-TurboID
Plasmid#149415PurposeHepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorHas serviceAAV8InsertER-TurboID
UseAAVTagsHA and V5PromoterTBGAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CMV-FLEX-SaCas9-U6-sgRosa26
Plasmid#159914PurposeMutagenesis of Rosa26 with SauCas9DepositorInsertRosa26 gRNA (Gt(ROSA)26Sor Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBOB-CAG-SARS-CoV2-Spike D614G-HA
Plasmid#158761Purpose3rd Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein D614G high infectivity mutant with a C-terminal HA tag generated by Junko Ogawa & Gerald M PaoDepositorInsertSARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
UseLentiviralTagsHAExpressionMammalianMutationSpike D614GPromoterCAGAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hsyn-GRAB_Ado1.0
Plasmid#153285PurposeExpress the adenosine sensor GRAB_Ado1.0 in neuronsDepositorHas serviceAAV1InsertAdenosine sensor GRAB_Ado1.0
UseAAVPromoterhSynAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV R-CEPIA4mt
Plasmid#140463PurposeRed fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertR-CEPIA4mt
TagsMycExpressionMammalianAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual-M2
Plasmid#135584PurposeHigh eukaryotic dual protein expression shuttle vectorDepositorTypeEmpty backboneUseTransfer vectorExpressionInsectPromoterpolyhedrin of AcMNPVAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE
Plasmid#139090PurposeExpresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.DepositorInsertdSpCas9-VPR
UseCRISPR and LentiviralExpressionMammalianMutationD10A, H840AAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ALKBH5-dCas9
Plasmid#134783PurposeExpression fusion protein ALKBH5-dCas9 in Mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertALKBH5 and inactive dCas9
ExpressionMammalianPromoterCMVAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-WT
Plasmid#133753PurposeWild type human TDP-43 in pLVX-Puro lentiviral vectorDepositorAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
R4pGWB601_UBQ10p-Turbo-NES-YFP
Plasmid#127366PurposeBinary vector for expressing cytosolic TurboID-YFP under the UBQ10 promoter in plantsDepositorInsertTurboID (BirA mutant)
TagsGS linker, NES, V5, and YFPExpressionPlantMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTRIP-SFFV-EGFP-FLAG-cGAS
Plasmid#127661PurposeOver-express EGFP-FLAG-cGASDepositorAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-8xHis-Ub
Plasmid#107392PurposeMammalian expression 8xHis-UbDepositorInsert8 tandem repeats of 6xHis-Ub (See comments below)
TagsNoneExpressionMammalianPromoterCMVAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SMARCA2-GFP
Plasmid#65390PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSMARCA2 (SMARCA2 Human)
TagsEmGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
GATA3-pCDNA3.1
Plasmid#115271PurposeExpresses human GATA3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGATA3 (GATA3 Human)
ExpressionMammalianPromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZS165 CRISPEY HH-HDV NotI
Plasmid#114451PurposeYeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HH-HDV ribozymeDepositorTypeEmpty backboneUseCRISPR; Cen/arsExpressionYeastPromoterScGAL7pAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI
Plasmid#115267PurposeExpresses LbuCas13a from a SUMO-tagged bacterial expression constructDepositorInsertLbuCas13a
TagsSUMOExpressionBacterialAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-dLight1.2
Plasmid#111068PurposeTo generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoterDepositorHas serviceAAV1 and AAV5InsertdLight1.2
UseAAVTagsFlag tagAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEMT-PTE2A
Plasmid#111773PurposeQuadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2ADepositorTypeEmpty backboneUseCloning intermediatePromoterT7Available sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX601 miniCMV-SaCas9-SpA-sgRNA scaffold
Plasmid#107055PurposeBackbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)DepositorTypeEmpty backboneUseAAV and CRISPRExpressionMammalianAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by