159,557 results

1. pNFkB:eGFP

   Plasmid

   #44922

   Depositor

   John Rawls

   Article

   Kanther et al Gastroenterology. 2011 Jul;141(1):197-20

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tet-pLKO-puro-SOX2

   Plasmid

   #47540

   Purpose

   Tet inducible knockdown of SOX2.

   Depositor

   Charles Rudin

   Article

   Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

   Insert

   SOX2 shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG mito-GCaMP5G

   Plasmid

   #105009

   Purpose

   mitochondrial calcium sensor

   Depositor

   Franck Polleux

   Article

   Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

   Insert

   GCaMP5G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21a-MetRS-His

   Plasmid

   #124115

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Methionine--tRNA ligase (metG Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. CL20_mEGFP_NDRG1_ERG

   Plasmid

   #205866

   Purpose

   Express mEGFP-tagged fusion protein, NDRG1_ERG from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   NDRG1_ERG (NDRG1, ERG Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 AIF 1-90 mCherry FRB

   Plasmid

   #67530

   Purpose

   For expression of mCherry fused FRB to the mitochondrial innermembrane

   Depositor

   Stephen Tait

   Article

   Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

   Inserts

   AIF a.a. 1 - 90
   mCherry
   FRB
   

   Use

   Tags

   AIF 1 - 90, FRB, and mCherry

   Expression

   Mammalian

   Mutation

   T->L

   Promoter

   CMV

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. ECFP-Bcr

   Plasmid

   #36415

   Depositor

   John Groffen, Nora Heisterkamp

   Article

   Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

   Insert

   breakpoint cluster region (BCR Human)
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

8. Cys-3HB-SPYcatcher

   Plasmid

   #214838

   Purpose

   This plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al bioRxiv 2024.01.21.576499.

   Insert

   Cys-3HB-SPYcatcher
   

   Use

   Tags

   His-TEV-SUMO-

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpRY-ABE8e(V106W)

   Plasmid

   #198553

   Purpose

   Expresses human codon-optimized SpRY-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpRY-ABE8e(V106W)-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpRY-ABE8e(V106W)
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFPL-Ch

    Plasmid

    #61647

    Purpose

    Gateway vector for generating cyan fluorescent protein fusions in fungi

    Depositor

    Mark Farman

    Article

    Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

    Insert

    enhanced cyan fluorescent protein
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF1168

    Plasmid

    #143472

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EBF2 (EBF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBBR-Cas9ΔyqhD-HA500

    Plasmid

    #220369

    Purpose

    A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

    Depositor

    Yilan Liu

    Inserts

    SpCas9
    sgRNA for yqhD in P. putida KT2440
    

    Use

    CRISPR; Broad host range

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-FlipCherry-T2A-GFP

    Plasmid

    #124436

    Purpose

    Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipCherry-T2A-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. L4440_BioBrick-hsf-1-sgRNA-A

    Plasmid

    #177786

    Purpose

    Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    hsf-1 (hsf-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. GST-PS-Grb2 (XSB363)

    Plasmid

    #78505

    Purpose

    Construct for expressing Grb2 in E. Coli

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    Grb2 (GRB2 Human)
    

    Use

    Tags

    GST followed by a prescission protease claevage s…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. px551-mSncg-0.27kb-HA-SpCas9

    Plasmid

    #153208

    Purpose

    Truncated Mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates HA and Cas9 expression in retinal ganglion cells

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Insert

    Cas9
    

    Use

    AAV and CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated mSncg (0.27kb)

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF3082

    Plasmid

    #144558

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GBX2 (GBX2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNP1 sfGFP toehold switch

    Plasmid

    #107355

    Purpose

    Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold 7 + sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHIV-H2B-eGFP

    Plasmid

    #91776

    Purpose

    Expresses H2B protein tagged with eGFP

    Depositor

    Maria Pia Cosma

    Article

    Sottile et al Sci Rep. 2016 Nov 9;6:36863. doi: 10.103

    Insert

    Human Histone H2B / eGFP (H2BC11 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGTag-TagRFP-SV40

    Plasmid

    #117807

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    TagRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. Fuw-dCas9-Dnmt3a

    Plasmid

    #84476

    Purpose

    Lentiviral construct to express dCas9 fused with Dnmt3a

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a (DNMT3A Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCIP-AMPKa1_KD

    Plasmid

    #79011

    Purpose

    For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    AMPK alpha 1 (PRKAA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K47R

    Promoter

    pCAGGs

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a-Rx3(A7)

    Plasmid

    #35525

    Depositor

    Robert Cohen

    Article

    Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

    Insert

    His6-Rx3(A7)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Changed G2 to P

    Promoter

    T7

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMAL-c4X

    Plasmid

    #75288

    Purpose

    Expresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

    Depositor

    Paul Riggs

    Article

    Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

    Type

    Empty backbone

    Use

    Tags

    maltose-binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 19, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pLminP_Luc2P_RE29

    Plasmid

    #90371

    Purpose

    SREBF1 - Sterol gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SREBPb

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHR-TRE3G-PYL1-sfGFP-PML

    Plasmid

    #167011

    Purpose

    expression of PYL1-SfGFP-PML, lentivirus vector

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    PML (PML Human)
    

    Use

    Lentiviral

    Tags

    GFP-PML

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMA4008

    Plasmid

    #70109

    Purpose

    mtDNA destruction/production of rho-0 cells

    Depositor

    Mikhail Alexeyev

    Article

    Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

    Inserts

    EGFP
    Herpes simplex virus UL12.5M185
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    N-terminal deletion of UL12.5

    Promoter

    CMV and RSV

    Available since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pm-iATPSnFR1.0

    Plasmid

    #102548

    Purpose

    membrane displayed ATP sensor for mammalian cells

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pYS16

    Plasmid

    #202082

    Purpose

    Parent vector for producing universal minicircle donors for mNeonGreen C-terminal knock-in

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    CT mNG-T2A-PuroR Minicircle Parent
    

    Use

    CRISPR

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    None

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBIG1a

    Plasmid

    #80611

    Purpose

    pBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge