162,109 results

1. pCAG-Cre-IRES2-GFP

   Plasmid

   #26646

   Purpose

   Mammalian coexpression of nls-Cre and EGFP from the CAG promoter

   Depositor

   Anjen Chenn

   Article

   Woodhead et al J Neurosci. 2006 Nov 29;26(48):12620-30.

   Insert

   Cre
   

   Use

   Cre/Lox

   Tags

   GFP and IRES2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. enAsABE8e

   Plasmid

   #138506

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-dAsCas12a (En)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1 3xHA-KillerRed-OMP25

   Plasmid

   #174544

   Purpose

   localizes KillerRed to mitochondria

   Depositor

   Minna Roh-Johnson

   Article

   Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

   Insert

   3xHA-KillerRed-OMP25
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPEgRNA

   Plasmid

   #172716

   Purpose

   Delivery of guide RNA for prime editing

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLPCX2-MT1-MMP-eGFP

   Plasmid

   #89819

   Purpose

   Express in MT1-MMP mammalian cells

   Depositor

   Shengyu Yang

   Article

   Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

   Insert

   MT1-MMP (MMP14 Human)
   

   Use

   Retroviral

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MMLVgag-3NES-Cre

   Plasmid

   #207252

   Purpose

   Generation of CreVLPs when used in conjunction with MMLV gag-pol during production

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   MMLVgag-3NES-Cre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF1a- gfp-2a-puro

   Plasmid

   #129443

   Purpose

   expression of GFP and Puro R

   Depositor

   Oskar Laur

   Insert

   gfp-puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-EGFP-Cdc42(Q61L)

   Plasmid

   #12600

   Depositor

   Klaus Hahn

   Article

   Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

   Insert

   EGFP-Cdc42 (Q61L) (CDC42 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q61L

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB-CMPE-ePPEplus

   Plasmid

   #205246

   Purpose

   For plant prime editing in wheat plants

   Depositor

   Zong Yuan

   Article

   Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

   Insert

   Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Detailed in manuscript

   Promoter

   Available since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCK122 pDESTattB_exorh:mCherry

    Plasmid

    #213511

    Purpose

    attB-containing pDEST plasmid, for use in Multisite Gateway reaction

    Depositor

    Christian Mosimann

    Article

    Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPP085

    Plasmid

    #158795

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAIP hIL4 co

    Plasmid

    #74169

    Purpose

    Lentiviral vector for expression of human codon optimized IL4 with puromycin resistance.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    IL4 (IL4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-Rubicon

    Plasmid

    #28022

    Depositor

    Qing Zhong

    Article

    Sun et al J Biol Chem. 2011 Jan 7. 286(1):185-91.

    Insert

    Rubicon (RUBCN Human)
    

    Use

    Tags

    3xFlag and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-HA-Parkin

    Plasmid

    #17613

    Depositor

    Ted Dawson

    Article

    Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.CAG.Flex.GCaMP6f.WPRE.SV40

    Plasmid

    #100835

    Purpose

    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    CAG

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. ABE8.20-NG

    Plasmid

    #226857

    Purpose

    Expresses ABE8.20-NG base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8.20-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. p58sheLL

    Plasmid

    #37324

    Depositor

    John Schiller

    Article

    Kondo et al Virology. 2007 Feb 20;358(2):266-72. Epu

    Insert

    HPV58L1+L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40

    Plasmid

    #100853

    Purpose

    AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV Retrograde and AAV1

    Insert

    jRGECO1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT-HA-hM3D(Gq)

    Plasmid

    #45547

    Purpose

    Expresses hM3D (Gq) neuronal activator

    Depositor

    Bryan Roth

    Article

    Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

    Insert

    hM3D (Gq)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-2NLS-Cas9-6XMS2

    Plasmid

    #166033

    Purpose

    Expression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.

    Depositor

    Yujia Cai

    Article

    Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. SpCas9-CBE6c

    Plasmid

    #215821

    Purpose

    Express CBE6c (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6c-SpCas9-2xUGI (cas9 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLenti6.3/TO/CD9-GFP

    Plasmid

    #104402

    Purpose

    Expresses CD9 with mEmerald tag (NOT GFP) at the N terminal

    Depositor

    Jens Gruber

    Article

    Boker et al Mol Ther. 2018 Feb 7;26(2):634-647. doi:

    Insert

    CD9 (CD9 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pDEST-CMV-N-EGFP

    Plasmid

    #122842

    Purpose

    N-terminal EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pinducer 20 DN-KASH

    Plasmid

    #125554

    Purpose

    Tet-ON inducible lentivirus expressing mcherry-labeled DN KASH

    Depositor

    Daniel Conway

    Article

    Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

    Insert

    SYNE1 (SYNE1 Human)
    

    Use

    Lentiviral

    Tags

    mcherry

    Expression

    Mutation

    Truncated form: only the KASH domain of nesprin-…

    Promoter

    tetracycline responsive element

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pUCP20T-eyfp

    Plasmid

    #78466

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    eYFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSLTS

    Plasmid

    #59386

    Purpose

    Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Type

    Empty backbone

    Use

    Genome engineering

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

    Plasmid

    #74010

    Purpose

    Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

    Depositor

    Filippo Del Bene

    Article

    Di Donato et al Genome Res. 2016 Mar 8.

    Inserts

    5xUAS
    Cas9
    Cre
    U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIMAY*

    Plasmid

    #121441

    Purpose

    Allelic exchange plasmid with PheS* counter-selection for Staphylococcus aureus

    Depositor

    Angelika Grundling

    Article

    Schuster et al Microbiology. 2019 Apr 3. doi: 10.1099/m

    Type

    Empty backbone

    Use

    Escherichia coli/staphylococcus aureus shuttle ve…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FUGW-APOE-E4-T2A-mCherry

    Plasmid

    #190809

    Purpose

    Express APOE E4 allele from Ubiquitin C promoter, co-expressing mCherry

    Depositor

    Ronald Hart

    Insert

    APOE (APOE Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E4

    Promoter

    Ubi C

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pBiFC-VN173

    Plasmid

    #22010

    Purpose

    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    VN173
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Venus residus 1-172

    Promoter

    Available since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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