Skip to main content
Addgene
Showing: 9521 - 9560 of 23917 results

  6. pWJ68

    Plasmid
    #46571
    Purpose
    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    w-dcas9
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46571-plasmid-map-sequence-id-65847

    Enlarge

  13. ACE2 g1 + Cas9

    Plasmid
    #153011
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153011-plasmid-map-sequence-id-298673

    Enlarge

  14. pAAV-UbC-eGFP-F

    Plasmid
    #71545
    Purpose
    Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.
    Depositor
    Pantelis Tsoulfas
    Insert
    eGFP-F
    Use
    AAV
    Tags
    contains 20- amino-acid farnesylation signal from…
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    71545-plasmid-map-sequence-id-132622

    Enlarge

  32. MSCB-hDot1Lmut

    Plasmid
    #74174
    Purpose
    For packaging hDot1Lmut (catalytic activity dead) to retrovirus.
    Depositor
    Yi Zhang
    Article
    Okada et al Cell. 2005 Apr 22;121(2):167-78.
    Insert
    hDot1L (DOT1L Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    hDot1L 163 GSG to RCR mutation*
    Promoter
    Available since
    Aug. 23, 2016
    Availability
    Academic Institutions and Nonprofits only

  40. pNR291

    Plasmid
    #79555
    Purpose
    Output plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.
    Depositor
    Timothy Lu
    Article
    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.
    Inserts
    GFPmut3b
    mTagBFP
    mRFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79555-plasmid-map-sequence-id-148766

    Enlarge

Showing: 9521 - 9560 of 23917 results