20,205 results
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Plasmid#62732PurposeLentiviral Cre recombinase reporter; EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked RFP-STOP cassette and switches fluorescence from red (RFP) to green (GFP).DepositorInsertLoxP-DsRED-STOP-LoxP-eGFP
UseCre/Lox and LentiviralTagsLoxP sitesExpressionMammalianPromoterEF1-AlphaAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-hLC3B-pcDNA3.1
Plasmid#40827DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLC3B (MAP1LC3B Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti CMV GFP DEST (736-1)
Plasmid#19732Purpose3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expressionDepositorPublicationTypeEmpty backboneUseGateway Cloning, Lentiviral, and RNAiExpressionMammalianAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCX-OKS-2A
Plasmid#19771PurposeNon-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianMutationThree genes are connected with the foot-and-mouth…Available sinceJan. 5, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE
Plasmid#32702PurposeConditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.DepositorInserteGFP
UseCre/Lox and RetroviralExpressionMammalianAvailable sinceDec. 13, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVPT-tTR-KRAB
Plasmid#11642PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertmPGK, GFP, tTR-KRAB, Tet-on
UseLentiviralExpressionMammalianAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-GCaMP5G
Plasmid#31788Purposea single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGCaMP5G
Tags6xHis, T7 epitope, and Xpress tagExpressionMammalianPromoterCMVAvailable sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-NLS-Cas9-6xHis
Plasmid#62934PurposeExpression of NLS-Cas9-6xHis in bacterial cellsDepositorInsertNLS-Cas9-6xHis
Tags6xHis tag and NLSExpressionBacterialPromoterT7Available sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mTagBFP2-pBAD
Plasmid#54572PurposeLocalization: Protein Expression Vector , Excitation: 399, Emission: 456DepositorTypeEmpty backboneTags6xHis, T7, and mTagBFP2ExpressionBacterialAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pLX302
Plasmid#25896Purpose3rd generation lentiviral Gateway destination vector. Puromycin selection, V5 tag.DepositorTypeEmpty backboneUseGateway Cloning and LentiviralTagsV5ExpressionMammalianAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-TurboID
Plasmid#124646PurposeExpresses Flag-TurboID fusion for proximity labelingDepositorTypeEmpty backboneTagsFlagExpressionMammalianPromoterCMVAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO5.sgRNA.EFS.GFP
Plasmid#57822Purpose3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter drivenDepositorInsertssgRNA
EFS
eGFP
UseCRISPR and LentiviralAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-Q74
Plasmid#40262DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHTT partial exon 1 Q74 (HTT Human)
TagsEGFPExpressionMammalianMutationexpanded poly-QPromoterCMVAvailable sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mito-V5-APEX2
Plasmid#72480PurposeExpresses APEX2 in mitochondriaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAPEX2
TagsV5ExpressionMammalianPromoterCMV promoterAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EF.CMV.RFP
Plasmid#17619DepositorPublicationTypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-EGFP-RhoA-wt
Plasmid#12965DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRhoA (RHOA Human)
TagsEGFPExpressionMammalianAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-HA
Plasmid#128034PurposeMammalian expression vector with N terminal HA tagDepositorTypeEmpty backboneTagsHAExpressionMammalianAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Inducible Caspex expression
Plasmid#97421PurposeFor stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomicsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCaspex
T2A-Gfp
UseCRISPRTags3xFLAG and V5ExpressionMammalianMutationdCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …PromoterTRE3GAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PX551
Plasmid#60957PurposepAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.DepositorInsertSpCas9
UseAAV and CRISPRTagsHAExpressionMammalianPromoterpMecp2Available sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCV-IRES-GFP II (pMIG II)
Plasmid#52107Purposea bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFPDepositorPublicationTypeEmpty backboneUseRetroviralTagsIRES-EGFPExpressionMammalianAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by