165,947 results

1. pGEM4Z-T7-5'UTR-EGFP-3'UTR-A64

   Plasmid

   #203348

   Purpose

   Template for T7 in vitro transcription of EGFP mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

   Depositor

   Christopher Grigsby, Molly Stevens

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Template for t7-mediated in vitro transcription o…

   Tags

   Flanked by 5' and 3' UTRs from Xenopus …

   Promoter

   T7

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJMP1339

   Plasmid

   #119271

   Purpose

   for cloning new sgRNAs

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. EJ2GFP-puro

   Plasmid

   #44025

   Depositor

   Jeremy Stark

   Article

   Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

   Insert

   EJ2GFP egfp-based chromosomal break reporter
   

   Expression

   Mammalian

   Mutation

   insertion of I-SceI, 3 frame stop, and flanking m…

   Promoter

   pCAGGS

   Available Since

   May 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cre Reporter

   Plasmid

   #62732

   Purpose

   Lentiviral Cre recombinase reporter; EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked RFP-STOP cassette and switches fluorescence from red (RFP) to green (GFP).

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   LoxP-DsRED-STOP-LoxP-eGFP
   

   Use

   Cre/Lox and Lentiviral

   Tags

   LoxP sites

   Expression

   Mammalian

   Promoter

   EF1-Alpha

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. UBC-4xmNG-dMCP

   Plasmid

   #231093

   Purpose

   Encodes a 4xmNeonGreen-dMCP under control of a weak promoter (UBC).

   Depositor

   John Ngo

   Article

   Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

   Insert

   4xmNG-dMCP
   

   Expression

   Mammalian

   Available Since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. BK Dunlop

   Plasmid

   #25466

   Depositor

   Peter Howley

   Insert

   human papovavirus BK Dunlop
   

   Expression

   Bacterial

   Available Since

   Sept. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pmPB2-VSV

   Plasmid

   #68038

   Purpose

   Expresses mouse Plexin-B2 with N-terminal VSV tag in mammalian cells

   Depositor

   Roland Friedel

   Article

   Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

   Insert

   Plexin-B2 (Plxnb2 Mouse)
   

   Tags

   VSV

   Expression

   Mammalian

   Available Since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PEmax-P2A-GFP

   Plasmid

   #180020

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax-P2A-EGFP
   

   Tags

   P2A-EGFP, SV40 bpNLS, and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEVOL-pAzF

   Plasmid

   #31186

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG

   Depositor

   Peter Schultz

   Article

   Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7

   Insert

   M.j. p-azidohenylalanine RS (2 copies +tRNA)
   

   Expression

   Bacterial

   Mutation

   Y32T, E107N, D158P, I159L, L162Q, D286R

   Available Since

   Dec. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. [#GS15-LV] DsRed GFP TEVp Blast Switch - Recipient

    Plasmid

    #233506

    Purpose

    Lentiviral construct for the MitoTRACER genetic reporter to be expressed in the recipient cells

    Depositor

    Simon Grelet

    Article

    Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

    Insert

    DsRed loxp eGFP
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas9

    Plasmid

    #42876

    Purpose

    Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    tracr/Cas9
    

    Use

    CRISPR; E.coli

    Expression

    Bacterial

    Available Since

    Feb. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJFRC19-13XLexAop2-IVS-myr::GFP

    Plasmid

    #26224

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    myr::GFP
    

    Expression

    Insect

    Available Since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBOB-EF1-FastFUCCI-Puro

    Plasmid

    #86849

    Purpose

    3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection

    Depositor

    Kevin Brindle, Duncan Jodrell

    Article

    Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164

    Inserts

    mKO2-hCDT1(30-120)
    mAG-hGEM(1-110)
    pac (puromycin N-acetyl-transferase)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1 and PGK

    Available Since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. hSTARR-seq_ORI vector

    Plasmid

    #99296

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Expression

    Mammalian

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. [#GS16-LV] Syn1-MitoTRACER - Donor

    Plasmid

    #233565

    Purpose

    Lentiviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells under the Synapsin1 promotor

    Depositor

    Simon Grelet

    Article

    Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

    Insert

    MitoTRACER-Donor
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    GFP11 and HA Tag

    Expression

    Mammalian

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. ppyCAG_RNaseH1_WT

    Plasmid

    #111906

    Purpose

    Express RNASEH1 in mammalian cell.

    Depositor

    Xiang-Dong Fu

    Article

    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

    Insert

    RNASEH1 (RNASEH1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    July 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-mNG-IRES-mScarlet-dPCP

    Plasmid

    #231090

    Purpose

    Bicistronic construct encoding mNG in combination with mScarlet-dPCP via an EMCV-IRES sequence.

    Depositor

    John Ngo

    Article

    Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

    Insert

    mNG-IRES-mScarlet-dPCP
    

    Expression

    Mammalian

    Available Since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Ef1a-fDIO hChR2(H134R)-EYFP

    Plasmid

    #55639

    Purpose

    Flp-Dependent ChR2-EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde

    Insert

    hChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-mCherry

    Plasmid

    #36084

    Purpose

    3rd generation lentiviral plasmid expressing mCherry

    Depositor

    Pantelis Tsoulfas

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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