We narrowed to 1,093 results for: Amot;

1. pPBbsr2-Necl-5-ScNeo

   Plasmid

   #170283

   Purpose

   To monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

   Insert

   a recombinant mouse Necl-5 fused to the mScarlet and to the mNeonGreen fluorescent protein (Pvr Synthetic, Mouse)
   

   Use

   Tags

   mScarlet and mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Raichu-OsRac1 CA

   Plasmid

   #133264

   Purpose

   Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

   Depositor

   Yoji Kawano

   Article

   Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

   Insert

   OsRac1(G19V) (LOC4325879 Oryza sativa)
   

   Use

   Tags

   CFP-lipid and Venus-CRIB

   Expression

   Plant

   Mutation

   OsRac1 G19V

   Promoter

   Ubi

   Available since

   Jan. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Raichu-OsRac1 DN

   Plasmid

   #133263

   Purpose

   Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

   Depositor

   Yoji Kawano

   Article

   Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

   Insert

   OsRac1(T24N) (LOC4325879 Oryza sativa)
   

   Use

   Tags

   CFP-lipid and Venus-CRIB

   Expression

   Plant

   Mutation

   OsRac1 T24N

   Promoter

   Ubi

   Available since

   Jan. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pbG-APRT-J-u32uni-PdTKmCh

   Plasmid

   #107279

   Purpose

   APRT c.407T>C (APRT*J) donor vector with unilateral microhomology

   Depositor

   Knut Woltjen

   Article

   Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

   Inserts

   APRT (APRT Human)
   puro-deltaTK; CAG::mCherry
   

   Use

   Tags

   Expression

   Mutation

   c.402A>T, c.407T>C

   Promoter

   Available since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-CDC27 wt

   Plasmid

   #31673

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   cell division cycle 27 homolog (CDC27 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

6. lenti-HA-mBec1

   Plasmid

   #99505

   Purpose

   3rd generation lentiviral expression of wild-type Becn1

   Depositor

   Congcong He

   Article

   Rocchi et al PLoS Genet. 2017 Aug 14;13(8):e1006962.

   Insert

   Beclin 1 (Becn1 Mouse)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMRX-IP-hWIPI1A-3×FLAG

   Plasmid

   #215509

   Purpose

   Expresses FLAG tagged human WIPI1A.

   Depositor

   Noboru Mizushima

   Article

   Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

   Insert

   WD-repeat protein interacting with phosphoinositides 1A (WIPI1 Human)
   

   Use

   Retroviral

   Tags

   3×FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-EF1a-CCre-MBP4-WPRE

   Plasmid

   #193919

   Purpose

   Expresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)

   Depositor

   Tatsushi Onaka

   Article

   Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

   Insert

   CCre-MBP4
   

   Use

   AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-EF1a-NCre-MBP5-WPRE

   Plasmid

   #193918

   Purpose

   Expresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)

   Depositor

   Tatsushi Onaka

   Article

   Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

   Insert

   NCre-MBP5
   

   Use

   AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXs-hGLIS1GR

    Plasmid

    #59312

    Purpose

    Forced expression of human GLIS1GR

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

    Insert

    GLIS1 (GLIS1 Human)
    

    Use

    Retroviral

    Tags

    cGR - hormone binding domain of glucocorticoid re…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLR5-CAG-NLS-tdiRFP670-2A-LBR

    Plasmid

    #82620

    Purpose

    Expresses NLS-tdiRFP-2A-LBR in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Insert

    NLS-tdiRFP670-LBR (Lbr Synthetic, Mouse)
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-CCre-MBP1-WPRE

    Plasmid

    #193917

    Purpose

    Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

    Insert

    CCre-MBP1
    

    Use

    AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-EF1a-NCre-MBP6-WPRE

    Plasmid

    #193916

    Purpose

    Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

    Insert

    NCre-MBP6
    

    Use

    AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pECE-M2-BAIAP2 S366A

    Plasmid

    #31657

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    BAI1-associated protein 2 S366A (BAIAP2 Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    changed Serine 366 to Alanine

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

15. p3-HPRT1-S104R-PdTK-mCh

    Plasmid

    #107276

    Purpose

    HPRT1 c.312C>A Munich donor vector with unilateral microhomology

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Inserts

    HPRT genomic DNA (HPRT1 Human)
    puro-deltaTK; CAG::mCherry
    

    Use

    Tags

    Expression

    Mutation

    c.306G>T, c.312C>A

    Promoter

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCSIIneo-TRE-hPhyB621- mCherry-HRasCT

    Plasmid

    #139482

    Purpose

    Expresses PhyB621-mCherry-HRasCT in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    PhyB621 (PHYB Mustard Weed)
    

    Use

    Lentiviral

    Tags

    mCherry-HRasCT

    Expression

    Mammalian

    Mutation

    G229L and deletion of aa Y at position 235 in mch…

    Promoter

    Tet responsible element

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p3-HPRT1-S104Rf-PdTK-mCh

    Plasmid

    #107277

    Purpose

    HPRT1 c.312C>A (Munich) donor vector with bilateral microhomology

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Inserts

    HPRT genomic DNA (HPRT1 Human)
    puro-deltaTK; CAG::mCherry
    

    Use

    Tags

    Expression

    Mutation

    c.306G>T, c.312C>A

    Promoter

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FUW-M2-PPP1R12C S452A

    Plasmid

    #31679

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    Protein Phosphatase 1 Regulatory Subunit 12C S452A (PPP1R12C Human)
    

    Use

    Lentiviral

    Tags

    M2

    Expression

    Mammalian

    Mutation

    changed Serine 452 to Alanine

    Promoter

    Available since

    April 12, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pGEX-BAIAP2 S366A

    Plasmid

    #31676

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    BAI1-associated protein 2 S366A (BAIAP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed Serine 366 to Alanine

    Promoter

    Available since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-HA Set1

    Plasmid

    #192219

    Purpose

    It expresses HA-fused mouse Set1 isoform protein in mammalian cells

    Depositor

    Hisakazu Ogita

    Article

    Molla et al Commun Biol. 2022 Oct 7;5(1):1071. doi:

    Insert

    Set isoform 1 (Set Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-HA Set2

    Plasmid

    #192220

    Purpose

    It expresses HA-fused mouse Set2 isoform protein in mammalian cells

    Depositor

    Hisakazu Ogita

    Article

    Molla et al Commun Biol. 2022 Oct 7;5(1):1071. doi:

    Insert

    Set isoform 2 (Set Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMRXIP-GFP-TAPP1(PHD)

    Plasmid

    #221757

    Purpose

    Stable expression of GFP-TAPP1(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    tandem TAPP1 (PHD) (amino acids 184-304)

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMRXIP-Btk1(PHD)-GFP

    Plasmid

    #221756

    Purpose

    Stable expression of Btk1(PHD)-GFP in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    Btk (BTK Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    amino acids 1–177

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMRXIP-GFP-ING2(PHD)

    Plasmid

    #221751

    Purpose

    Stable expression of GFP-ING2(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    ING2 (ING2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    two ING2(PlantHD) domains (amino acids 190–280)

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNM1325

    Plasmid

    #197144

    Purpose

    CAGp-Target-AID-2A-BlastR for PiggyBac genomic integration

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Insert

    SpCas9-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SpCas9(D10A)

    Promoter

    CAG

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-CS-196

    Plasmid

    #197138

    Purpose

    CloneSelect C-to-T mCherry (M9A) ATG control reporter encoding BC-C1 barcode

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV-CS-117

    Plasmid

    #197134

    Purpose

    CloneSelect C-to-T reporter encoding three target barcodes (3-input OR gate circuit)

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    EGFP(M1V)

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLV-CS-060

    Plasmid

    #197133

    Purpose

    CRISPRa reporter cloning backbone and MCP-p65-HSF1-2A-Puro expression

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    MCP(N55K)

    Promoter

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV-CS-193

    Plasmid

    #197137

    Purpose

    CloneSelect C-to-T mCherry (M1V/M9A) reporter encoding BC-C1 barcode

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    mCherry(M1V/M9A)

    Promoter

    CMV

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV-CS-119

    Plasmid

    #197135

    Purpose

    CloneSelect A-to-G reporter encoding three target barcodes (3-input AND gate circuit)

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    EGFP(M1V)

    Promoter

    CMV

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. eSpCas9-EF-5Flnc

    Plasmid

    #174870

    Purpose

    CRISPR vector for generating Flnc STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Flnc (Flnc Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLenti6.2-coHPDL H163A H258A 3xFLAG-V5

    Plasmid

    #174131

    Purpose

    Expresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cells

    Depositor

    Michael Pacold

    Article

    Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

    Insert

    4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Codon optimized, H163A, H258A

    Promoter

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLenti6.2-coHPDL H258A 3xFlag-V5

    Plasmid

    #174130

    Purpose

    Expresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cells

    Depositor

    Michael Pacold

    Article

    Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

    Insert

    4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Codon optimized, H258A

    Promoter

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMRXIP-GFP-TRPML1(PHD)

    Plasmid

    #221752

    Purpose

    Stable expression of GFP-TRPML1(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    TRPML1 (MCOLN1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    tandem TRPML1(PHD) (amino acids 1–69)

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-GFP-LC3-RFP-LC3ΔG

    Plasmid

    #168997

    Purpose

    Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV enAsCas12f-HKRA-sgRNA_v7

    Plasmid

    #208357

    Purpose

    Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

    Depositor

    Atsushi Hoshino

    Article

    Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

    Insert

    enAsCas12f-HKRA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I123H/D195K/D208R/V232A

    Promoter

    CMV

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMRX-IP-GFP-LC3-RFP

    Plasmid

    #84573

    Purpose

    Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV enAsCas12f-YHAM-sgRNA_v7

    Plasmid

    #208356

    Purpose

    Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

    Depositor

    Atsushi Hoshino

    Article

    Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

    Insert

    enAsCas12f-YHAM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F48Y/S188H/V232A/E316M

    Promoter

    CMV

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pW17

    Plasmid

    #158207

    Purpose

    2lox landing pad plasmid with mariner transposon and transposase used for CRAGE

    Depositor

    Yasuo Yoshikuni

    Article

    Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

    Insert

    loxP/Cre/KmR/Lox5171/Mariner IR oriT/transposase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. CTCF-mAC donor (Hygro)

    Plasmid

    #140646

    Purpose

    CTCF tagging with mAID-clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CTCF (CTCF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge