We narrowed to 22,188 results for: ESC

1. pAAV-CMV-SpCas9D10A nickase

   Plasmid

   #216737

   Purpose

   Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CMVd1

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-Syn-miRFP

   Plasmid

   #108416

   Purpose

   Monomeric near-infrared fluorescent protein miRFP in mammalian-expressing AAV production plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   miRFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-FLAG-RPS14(WT)

   Plasmid

   #122297

   Purpose

   expresses RPS14 protein with a flag tag

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RPS14 (RPS14 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-Myc-CyclinD1(WT)

   Plasmid

   #122300

   Purpose

   expresses Cyclin D1 protein with a Myc tag

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV(gRNA)-CMV-eGFP-U6(sgCTG)

   Plasmid

   #216732

   Purpose

   Contains a eGFP gene along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Used with the HD iPSC-derived astrocytes

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   sgCTG lentiviral guide RNA with eGFP tag
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBABE-RPS14(WT)-Myc

   Plasmid

   #122235

   Purpose

   expresses RPS14 protein with a Myc tag in mammalian cells (puro resistance)

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RPS14 (RPS14 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   vector's LTR

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-U6-sgCTG-CMV-GFP

   Plasmid

   #216734

   Purpose

   AAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   sgCTG
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and CMV

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-nEF-SpCas9D10A nickase

   Plasmid

   #216736

   Purpose

   Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   EF-1-alpha core

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTW5126

   Plasmid

   #129732

   Purpose

   polyhistidine replacement of EatA passenger DUF corresponding to amino acids E541 -Q617 of the native EatA protein

   Depositor

   James Fleckenstein

   Article

   Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do

   Insert

   eatA-DUF::His8
   

   Use

   Tags

   9His tag

   Expression

   Bacterial

   Mutation

   replaces the domain of unknown function correspon…

   Promoter

   araBAD

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MSCV-RPS14(WT)-Myc

    Plasmid

    #122236

    Purpose

    expresses RPS14 protein with a Myc tag in mammalian cells (hygro resistance)

    Depositor

    Gerardo Ferbeyre

    Article

    Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

    Insert

    RPS14 (RPS14 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

    Plasmid

    #216733

    Purpose

    Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase and sgCTG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    U6 and CAG

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX551-miniCMV-SpCas9D10A nickase

    Plasmid

    #216735

    Purpose

    Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    T7 and mini-CMV

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-U6-AlbEx14-mNG

    Plasmid

    #201780

    Purpose

    knocks in mNeonGreen into exon 14 of Alb to produce Alb-mNeonGreen fusion protein in mice

    Depositor

    Hajime Hirase

    Article

    Vittani et al Fluorescence Imaging of the Brain. Neuro

    Inserts

    Alb gRNA (Alb Mouse)
    Alb_LHA-mNeonGreen-Alb_RHA (Alb Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-FLAG-RPS14(89-151)

    Plasmid

    #122299

    Purpose

    expresses a part of RPS14 protein with a flag tag

    Depositor

    Gerardo Ferbeyre

    Article

    Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

    Insert

    RPS14 (RPS14 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1 to 88

    Promoter

    CMV

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-FLAG-RPS14(2-88)

    Plasmid

    #122298

    Purpose

    expresses a part of RPS14 protein with a flag tag

    Depositor

    Gerardo Ferbeyre

    Article

    Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

    Insert

    RPS14 (partial) (RPS14 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 89 to 151

    Promoter

    CMV

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TFORF1522

    Plasmid

    #143288

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR2E3 (NR2E3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TFORF1521

    Plasmid

    #144181

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR2E3 (NR2E3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC57-BBclone1

    Plasmid

    #137069

    Purpose

    E. coli plasmid that contains the expression optimized genes for BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Inserts

    BB0405 (BB_0405 Borrelia burgdorferi B31)
    BB0406
    P66
    BBA57
    HtrA
    RevA (revA Borrelia burgdorferi B31)
    BB0238
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wild-type, full-length (signal-peptide-containing…

    Promoter

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-EGFP

    Plasmid

    #13031

    Purpose

    Mammalian expression vector for cloning your gene fused to EGFP

    Depositor

    Doug Golenbock

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBAD-mLemon

    Plasmid

    #191256

    Purpose

    Bacterial expression plasmids for pH sensitive fluorescent protein mLemon

    Depositor

    Robert Campbell

    Insert

    mLemon
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAIP4_5M2Y

    Plasmid

    #234193

    Purpose

    Heterologous protein expression of B7LG64_ECO55 in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5M2Y
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pLenti6.2_miRFP670

    Plasmid

    #113726

    Purpose

    Expresses the fluorescent protein miRFP670 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Insert

    miRFP670
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBAD-pHmGL

    Plasmid

    #191257

    Purpose

    Bacterial expression plasmids for pH sensitive fluorescent protein pHmGL

    Depositor

    Robert Campbell

    Insert

    pHmGL
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJK515

    Plasmid

    #72385

    Purpose

    Produces Escherichia coli glycinamide ribonucleotide formyltransferase 2 (EcPurT)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylglycinamide formyltransferase 2
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMflT-o4-mNG

    Plasmid

    #201855

    Purpose

    Mesoplasma florum oriC plasmid expressing mNeonGreen fluorescent protein

    Depositor

    Sébastien Rodrigue

    Insert

    mNeonGreen
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMflT-o4-YFP

    Plasmid

    #201856

    Purpose

    Mesoplasma florum oriC plasmid expressing LanYFP fluorescent protein

    Depositor

    Sébastien Rodrigue

    Insert

    LanYFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-CFP

    Plasmid

    #13030

    Purpose

    Mammalian expression vector for cloning your gene fused to CFP

    Depositor

    Doug Golenbock

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti6.2_mTurquoise2

    Plasmid

    #113723

    Purpose

    Expresses the fluorescent protein mTurquoise2 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Insert

    mTurquoise2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBAD-pHScarlet

    Plasmid

    #191258

    Purpose

    Bacterial expression plasmids for pH sensitive fluorescent protein pHScarlet

    Depositor

    Robert Campbell

    Insert

    pHScarlet
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti6.2_TagBFP

    Plasmid

    #113724

    Purpose

    Expresses the fluorescent protein TagBFP in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Insert

    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJK649

    Plasmid

    #72399

    Purpose

    Produces Escherichia coli phosphoribosylpyrophosphate amidotransferase (EcPurF)

    Depositor

    T. J. Kappock

    Insert

    amidophosphoribosyltransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-mRFP

    Plasmid

    #13032

    Purpose

    Mammalian expression vector for cloning your gene fused to mRFP

    Depositor

    Doug Golenbock

    Has Service

    Cloning Grade DNA

    Insert

    Monomer Red Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-YFP

    Plasmid

    #13033

    Purpose

    Mammalian expression vector for cloning your gene fused to YFP

    Depositor

    Doug Golenbock

    Has Service

    Cloning Grade DNA

    Insert

    Yellow fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAIP4_3A7L

    Plasmid

    #234182

    Purpose

    Heterologous protein expression of glycine cleavage system H protein in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3A7L (GCV3 Escherichia coli K-12)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. MBP-Benzonase

    Plasmid

    #218192

    Purpose

    Bacterial expression of a periplasmically expressed MBP-tagged Serratia Marcescens NucA (Benzonase); also known as c001

    Depositor

    Cheryl Arrowsmith

    Insert

    nucA
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 24, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pKSB- 3xP3-DsRedSV40 GGGG AAGA

    Plasmid

    #174532

    Purpose

    3xP3-DsRed fluorescence marker cassette for gene knock-in in mosquitoes

    Depositor

    Eric Marois

    Insert

    3xP3-DsRed BsaI cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mOrange in pBSDONR P4r-P2

    Plasmid

    #121529

    Purpose

    For making C-terminal mOrange fusions using multisite Gateway cloning

    Depositor

    Roger Innes

    Insert

    mOrange
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAIP4_3EYE

    Plasmid

    #234176

    Purpose

    Heterologous protein expression of N-acetylgalactosamine-specific enzyme IIB component of PTS in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3EYE
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pJK639

    Plasmid

    #72398

    Purpose

    Produces Escherichia coli phosphoribosylpyrophosphate amidotransferase, C-terminal His6 tag (EcPurFH6)

    Depositor

    T. J. Kappock

    Insert

    amidophosphoribosyltransferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBlu-CGT-1

    Plasmid

    #170158

    Purpose

    Expresses bacterial Cyclomaltodextrin Glucanotransferase from Paenibacillus sp. c36 in E.coli

    Depositor

    Sarah MacDonald

    Insert

    Cyclomaltodextrin Glucanotransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBAD-cyScarlet

    Plasmid

    #191260

    Purpose

    Bacterial expression plasmids for Long Stokes shift red fluorescent protein cyScarlet

    Depositor

    Robert Campbell

    Insert

    cyScarlet
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. sCFP in pBSDONR P4r-P2

    Plasmid

    #121530

    Purpose

    For making C-terminal sCFP fusions using multisite Gateway cloning

    Depositor

    Roger Innes

    Insert

    sCFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pJK690

    Plasmid

    #72380

    Purpose

    Produces Escherichia coli monothiol glutaredoxin GrxD, N-terminal His6 tag (H6EcGrxD)

    Depositor

    T. J. Kappock

    Insert

    glutaredoxin-4
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAIP4_1TJE

    Plasmid

    #234203

    Purpose

    Heterologous protein expression of threonine--tRNA ligase (EC 6.1.1.3) (Threonyl-tRNA synthetase) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1TJE
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. pAIP4_1HKA

    Plasmid

    #234169

    Purpose

    Heterologous protein expression of 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1HKA (folK Escherichia coli)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

46. pGBR22-pGEM-T

    Plasmid

    #231055

    Purpose

    Expresses Montipora efflorescens GFP-like chromoprotein under T7 with a 6xHIS tag

    Depositor

    Josh Beckham

    Insert

    GFP-like chromoprotein
    

    Use

    Cloning

    Tags

    6x HIS

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pET-29b(+)_NSP4ss_SerM_NucA-His

    Plasmid

    #218190

    Purpose

    Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

    Depositor

    Florence Pojer

    Insert

    nucA
    

    Use

    Tags

    His and NSP4 signal secretion peptide

    Expression

    Bacterial

    Mutation

    codon optimized ecoli residues - 22-266

    Promoter

    T7

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pET-29b(+)_NSP4ss_SerM_NucA-myc

    Plasmid

    #218191

    Purpose

    Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)

    Depositor

    Florence Pojer

    Insert

    nucA
    

    Use

    Tags

    NSP4 signal secretion peptide and myc

    Expression

    Bacterial

    Mutation

    codon optimized ecoli residues - 22-266

    Promoter

    T7

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pLV-EBFP2-nuc

    Plasmid

    #36085

    Depositor

    Pantelis Tsoulfas

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Replace EYFP of the vector pEYFP-Nuc from Clontec…

    Promoter

    Available Since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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50. pQ2aB

    Plasmid

    #124887

    Purpose

    Basic Retroviral backbone with multiple 2a sites, linkers, BFP and and IRES_puromycin from pQCXIP

    Depositor

    Nevil Singh

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Blue Flourescent Protein, Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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