We narrowed to 2,351 results for: hal.2

1. AAV pCAG-mRuby3-WPRE

   Plasmid

   #107744

   Purpose

   Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

   Depositor

   Rylan Larsen

   Article

   Zhuang et al Cell Rep. 2021 Oct 12;37(2):109826. doi:

   Insert

   mRuby3
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFRT-DHX36iso2E335A

   Plasmid

   #159588

   Purpose

   Express N-ter FLAG HA tagged DHX36 isoform 2 with E335A mutation in mammalian cells

   Depositor

   Markus Hafner

   Article

   Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

   Insert

   DHX36 (DHX36 Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   E335A

   Promoter

   CMV

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMSCV-FKBP-IRSp53(trunc C2)

   Plasmid

   #221274

   Purpose

   Expression of IRSp53 C-terminal truncation 2 (247-335) in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   IRSp53 C-terminal truncation 2 (247-335) (Baiap2 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG-4xFKBP

   Expression

   Mutation

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lentiGuide-puro_Panc480-MT7

   Plasmid

   #200941

   Purpose

   Multiplexed CRISPR array expressing 7 sgRNA in a lentiviral backbone

   Depositor

   James Eshleman

   Article

   Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

   Insert

   multiplexed sgRNA array
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF-1a

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PHR-GFP-VP16

   Plasmid

   #183927

   Purpose

   Optogenetic PHR domain coupled to GFP and VP16 activation domain; binds to CIBN upon blue light exposure

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   PHR-GFP-VP16 (CRY2, UL48 Mustard Weed, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGL3b(1454/3172)P2P-wt

   Plasmid

   #21404

   Depositor

   Eric Metzen

   Article

   Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

   Insert

   EGLN1 (EGLN1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

7. LAMP-mCherry-CRY2

   Plasmid

   #102249

   Purpose

   Expresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)

   Depositor

   Bianxiao Cui

   Article

   Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

   Insert

   LAMP-mCherry-CRY2 (LAMP1, CRY2 Mustard Weed, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PEX-mCherry-CRY2

   Plasmid

   #102250

   Purpose

   Expresses fusion of CRY2PHR with mCherry and PEX3 (1-42)

   Depositor

   Bianxiao Cui

   Article

   Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

   Insert

   PEX-mCherry-CRY2 (PEX3, CRY2 Mustard Weed, Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PHR-GFP-FUSN-VP16

   Plasmid

   #183933

   Purpose

   Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   PHR-GFP-FUSN-VP16 (CRY2, UL48 Mustard Weed, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDGB3omega1_KanR_BastaR

    Plasmid

    #186426

    Purpose

    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Inserts

    KanR
    BastaR
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    CaMV 35S and Pnos

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mCherry-CRY2-P14P5K

    Plasmid

    #79570

    Purpose

    expression of mCherry-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1c

    Depositor

    Olof Idevall-Hagren

    Article

    Xie et al Cell Chem Biol. 2016 Jul 21;23(7):816-26

    Insert

    Pip5k1c (Pip5k1c Mouse)
    

    Use

    Tags

    CRY2 (photolyase homology region domain of Arabid…

    Expression

    Mammalian

    Mutation

    R445E and R446E; truncation of amino acids 636-66…

    Promoter

    CMV

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

12. GFP-CRY2-P14P5K

    Plasmid

    #79569

    Purpose

    expression of GFP-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1c

    Depositor

    Olof Idevall-Hagren

    Article

    Xie et al Cell Chem Biol. 2016 Jul 21;23(7):816-26

    Insert

    Pip5k1c (Pip5k1c Mouse)
    

    Use

    Tags

    CRY2 (photolyase homology region domain of Arabid…

    Expression

    Mammalian

    Mutation

    R445E and R446E; truncation of amino acids 636-66…

    Promoter

    CMV

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pGL3b(1454/3172)P2P-mut

    Plasmid

    #21405

    Depositor

    Eric Metzen

    Article

    Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

    Insert

    EGLN1 (EGLN1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    hypoxia response element mutated

    Promoter

    Available since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

14. TET1CD-CRY2-EGFP

    Plasmid

    #82555

    Purpose

    Encodes TET1 under LITE2.0 system

    Depositor

    Joseph Irudayaraj

    Article

    Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

    Inserts

    TET1 (TET1 Human)
    CRY2 (CRY2 Mustard Weed)
    EGFP
    

    Use

    AAV

    Tags

    2A, NLS(alpha-imp), and NLS-VP64

    Expression

    Mammalian

    Mutation

    CRY2PHR (N-terminal photolyase homology region do…

    Promoter

    EF-1alpha

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PX458_ATF4_2

    Plasmid

    #72352

    Purpose

    Encodes gRNA for 3' target of human ATF4 along with Cas9 with 2A GFP

    Depositor

    Richard M. Myers, Eric Mendenhall

    Insert

    ATF4 (ATF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PX458_ATF4_1

    Plasmid

    #72351

    Purpose

    Encodes gRNA for 3' target of human ATF4 along with Cas9 with 2A GFP

    Depositor

    Richard M. Myers, Eric Mendenhall

    Insert

    ATF4 (ATF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMi(3xP3-EGFP)

    Plasmid

    #102540

    Purpose

    Transgenesis vector based on the Minos transposon, carrying the 3xP3-EGFP marker

    Depositor

    Charalambos Savakis, Michalis Averof

    Article

    Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

    Insert

    Minos transpoable element with 3xP3-EGFP marker
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPHR-mCherry-FUSN-mHP1alpha

    Plasmid

    #181892

    Purpose

    Soluble light-dependent effector protein (HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN) ) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-FUSN-mHP1alpha (Cbx5 Mouse, Mustard Weed)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPHR-mCherry-PGL-1-mHP1alpha

    Plasmid

    #181894

    Purpose

    Soluble light-dependent effector protein (HP1alpha fused to PGL-1) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-PGL-1-mHP1alpha (Cbx5 Mouse, Mustard Weed)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPHR-mCherry-nE-mHP1alpha

    Plasmid

    #181890

    Purpose

    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-nE-mHP1alpha (Cbx5 Mouse, Mustard Weed)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPHR-mCherry-RGG-GFP-RGG-mHP1alpha

    Plasmid

    #181893

    Purpose

    Soluble light-dependent effector protein (HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-RGG-GFP-RGG-mHP1alpha (Cbx5 Mouse, Mustard Weed)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPHR-mCherry-nE-mHP1alpha-delCTE

    Plasmid

    #181891

    Purpose

    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-nE-mHP1alpha-delCTE (Cbx5 Mouse, Mustard Weed)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFETCh_ATF4

    Plasmid

    #72350

    Purpose

    Donor vector for 3' FLAG tag of human ATF4

    Depositor

    Richard M. Myers, Eric Mendenhall

    Insert

    ATF4 Homology Arms (ATF4 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDuEx2-NIR-GECO1

    Plasmid

    #113680

    Purpose

    Bacterial and mammalian expression plasmid for near-infrared fluorescent Ca2+ indicator NIR-GECO1

    Depositor

    Robert Campbell

    Article

    Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

    Insert

    NIR-GECO1
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-hSyn-NES-NIR-GECO1

    Plasmid

    #113683

    Purpose

    Express NIR-GECO1 preferentially in neurons

    Depositor

    Robert Campbell

    Article

    Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

    Insert

    NES-NIR-GECO1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. hSyn1-AsCpf1(RR)

    Plasmid

    #109325

    Purpose

    AAV vector expressing AsCpf1 (RR variant)

    Depositor

    Feng Zhang

    Article

    Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

    Insert

    hAsCpf1(RR)
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mutation

    S542R/K607R

    Promoter

    hSyn1

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pU6-crRNA(Non-targeting)

    Plasmid

    #109324

    Purpose

    AAV vector expressing non-targeting AsCpf1 crRNA

    Depositor

    Feng Zhang

    Article

    Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

    Insert

    mCherry-KASH
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TOPO-ZNF84

    Plasmid

    #226450

    Purpose

    For subcloning of human ZNF84 promoter or for assays using M13 phage

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    ZNF84 (ZNF84 Human)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    No

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TOPO-CHR12:132.68MB LG4

    Plasmid

    #226454

    Purpose

    Encodes an LG4 enhancer for subcloning or for assays using M13 phage

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    CHR12:132.68MB LG4
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    No

    Promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166014

    Purpose

    mammalian expression of HCoV-HKU1 soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    HCoV-HKU1 S (spike)
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. R619-M66-303: CMV51p> HCoV-OC43 S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166015

    Purpose

    mammalian expression of HCoV-OC43 soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    HCoV-OC43 S (spike)
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. R619-M91-303: CMV51p> HCoV-NL63 S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166017

    Purpose

    mammalian expression of HCoV-NL63 soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    HCoV-NL63 S (spike)
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. R619-M90-303: CMV51p> HCoV-229E S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166016

    Purpose

    mammalian expression of HCoV-229E soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    HCoV-229E S (spike) (S )
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. R619-M88-303: CMV51p> MERS-CoV S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166013

    Purpose

    mammalian expression of MERS-CoV soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    MERS-CoV S (spike)
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGFP-hPMLIII

    Plasmid

    #181903

    Purpose

    Fluorescently tagged PMLIII

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    hPMLIII (PML Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. R619-M87-303: CMV51p> SARS-CoV S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166012

    Purpose

    mammalian expression of SARS-CoV soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV S (spike)
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFUW-TetO-CREM

    Plasmid

    #178442

    Purpose

    doxycycline-inducible expression of Human CREM in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    CREM (CREM Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pdCas9-GFP

    Plasmid

    #181906

    Purpose

    Fluorescently tagged dead Cas9

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV-monSTIM1

    Plasmid

    #161795

    Purpose

    Mammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)

    Depositor

    Won Do Heo

    Article

    Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

    Insert

    MonSTIM1 (STIM1 Mustard Weed, Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. FcD265A-FLAG

    Plasmid

    #65420

    Purpose

    Mammalian expression plasmid for murine Fc-FLAG, used for monomeric Fc-IL2 production

    Depositor

    Dane Wittrup

    Article

    Zhu et al Cancer Cell. 2015 Apr 13;27(4):489-501.

    Insert

    FcD265A-FLAG
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D265A

    Promoter

    Modified CMV Promoter

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

41. pTRE-BI-aaAFB2

    Plasmid

    #207839

    Purpose

    Inducible AFB2 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

    Depositor

    Michaela Mueller-McNicoll

    Article

    Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

    Inserts

    atAFB2
    mCherry
    miniIAA7
    VHH-GFP4
    

    Use

    Tags

    3X FLAG-Tag and weak NLS

    Expression

    Mammalian

    Mutation

    All K mutated to R

    Promoter

    TRE3G BI promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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42. TOPO-LG4_chr5:550kb antisense

    Plasmid

    #205468

    Purpose

    Enhancer containing plasmid used for assessing enhancer::promoter interactions

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    chr5:550kb LG4
    

    Use

    Synthetic Biology; Subcloning of taq-amplified pc…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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43. pMSCV-FKBP-GMIP(trunc C2)

    Plasmid

    #221280

    Purpose

    Expression of GMIP C-terminal truncation 2 (1-44) in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    GMIP C-terminal truncation 2 (1-44) (Gmip Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG-4xFKBP

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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44. pCMV-OptoTGFBRs

    Plasmid

    #118942

    Purpose

    Mammalian expression plasmid of OptoTGFBRs (optogenetically-activated TGFb receptors)

    Depositor

    Won Do Heo

    Article

    Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

    Insert

    OptoTGFBRs (Tgfb1, Tgfbr2 Mouse, Mustard Weed)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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45. pLNCX2-OptoTGFBRs

    Plasmid

    #118965

    Purpose

    Retroviral expression OptoTGFBRs for transient or stable constitutive expression

    Depositor

    Won Do Heo

    Article

    Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

    Insert

    OptoTGFBRs (Tgfb1, Tgfbr2 Mouse, Mustard Weed)
    

    Use

    Retroviral

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. pET16-mHP1alpha

    Plasmid

    #181910

    Purpose

    Bacterial protein expression vector for HP1alpha

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1alpha (Cbx5 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. pET28-mHP1alpha

    Plasmid

    #181911

    Purpose

    Bacterial protein expression vector for HP1alpha

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1alpha (Cbx5 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pU6-crRNA(TBK1)

    Plasmid

    #109322

    Purpose

    AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TBK1

    Depositor

    Feng Zhang

    Article

    Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

    Insert

    mCherry-KASH (TBK1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. PGK_ACE2-ECD_Notch-Gal4VP64 (pZYW037)

    Plasmid

    #194226

    Purpose

    Lentiviral expression vector expressing ACE2-ECD SynNotch and mCherry transduction marker

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    PGK-ACE2-SynNotch-T2A-mCherry (ACE2 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. psgRNA-2xMS2-lacO

    Plasmid

    #181909

    Purpose

    Single guide RNA with 2XMS2 loops targeting bacterial lac operator

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    sgRNA-2XMS2-lacO
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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