59,541 results

1. pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin

   Plasmid

   #133028

   Purpose

   Expresses EGFP-tagged Golgi apparatus marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Golgin84 (GOLGA5 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-673

   Promoter

   Human elongation factor 1 alpha

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. LSB-hsa-let-7a-5p

   Plasmid

   #103146

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-let-7a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-let-7a-5p target
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Halo-Sec23A

   Plasmid

   #166892

   Purpose

   mammalian expression of Sec23A tagged with HaloTag

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   SEC23A (SEC23A Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-PspCas13b-GEMSgRNA2

   Plasmid

   #204768

   Purpose

   pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   guide RNA targeting GEMS m6A sensor mRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-hNurr1-NE

   Plasmid

   #102363

   Purpose

   Overexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cells

   Depositor

   Shu Leong Ho

   Insert

   NR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
   

   Tags

   NE

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMK463 (BSD-P2A-mAB)

   Plasmid

   #214389

   Purpose

   mAID-BromoTag for N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   BSD-P2A-mAID-BromoTag
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGfaABC1D-nLac

   Plasmid

   #53131

   Purpose

   expression of lacZ driven by human GfaABC1D promoter

   Depositor

   Michael Brenner

   Article

   Lee et al Glia. 2008 Apr;56(5):481-93. doi: 10.100

   Insert

   Gfa2 (GFAP Human)
   

   Use

   Mouse Targeting

   Tags

   LacZ, NLS, and mP1

   Expression

   Mammalian

   Mutation

   contains ABC1D regions of Gfa2 promoter, -1757 to…

   Promoter

   human Gfa2

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_NOTCH2_WT

   Plasmid

   #82292

   Purpose

   Gateway Donor vector containing NOTCH2, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   NOTCH2 (NOTCH2 Human)
   

   Use

   Gateway entry vector

   Mutation

   C946Y

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. NanoLuc-EIF2AK2 Fusion Vector

   Plasmid

   #238858

   Purpose

   Express NanoLuc-EIF2AK2 Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   EIF2AK2 (EIF2AK2 Human)
   

   Tags

   NanoLuc (R)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pAAVLINK-EF1alpha-5' NEXMIF

    Plasmid

    #236668

    Purpose

    5' AAVLINK plasmid for NEXMIF expression

    Depositor

    Zhonghua Lu

    Insert

    NEXMIF (NEXMIF Human)
    

    Use

    AAV

    Tags

    HA

    Promoter

    EF1alpha

    Available Since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAVLINKv1-pCALM1-Cre-3' NEXMIF

    Plasmid

    #236669

    Purpose

    3' AAVLINK plasmid for NEXMIF expression

    Depositor

    Zhonghua Lu

    Insert

    NEXMIF (NEXMIF Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG

    Promoter

    pCALM1

    Available Since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA -TMEM9B-3xHA

    Plasmid

    #225509

    Purpose

    TMEM9B expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

    Insert

    TMEM9B (TMEM9B Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAIP4_2ESK

    Plasmid

    #234232

    Purpose

    Heterologous protein expression of ubiquitin-conjugating enzyme E2 D2 in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2ESK (UBE2D2 Human)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pLenti puro HA-H2BC21

    Plasmid

    #227589

    Purpose

    Lentiviral plasmid expressing human H2BC21 protein

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    H2BC21 (H2BC21 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    CMV

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. GRM6-DuET

    Plasmid

    #213304

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GRM6 (GRM6 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTMB352_ZFcharm_Kv1_split-inteins

    Plasmid

    #220848

    Purpose

    Lentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFS

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    ZFcharm Kv2
    

    Use

    Lentiviral

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. hNCAM2

    Plasmid

    #84473

    Purpose

    expression of hNCAM2 in mammalian cells

    Depositor

    Vladimir Sytnyk

    Article

    Leshchyns'ka et al Nat Commun. 2015 Nov 27;6:8836. doi: 10.

    Insert

    NCAM2 (NCAM2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-TLR2-CFP

    Plasmid

    #13015

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 2 (TLR2 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. phDLX1-N174

    Plasmid

    #60859

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses DLX1 under the EF1a promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    DLX1 (DLX1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 Flag FKHR AAA mutant

    Plasmid

    #13508

    Depositor

    Kunliang Guan

    Article

    Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

    Insert

    FKHR AAA (FOXO1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T24A S256A S319A

    Available Since

    Dec. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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