159,284 results

1. pAAV2-CMV-GFP-BGHpA

   Plasmid

   #190239

   Purpose

   Expresses EGFP in mammalian cells

   Depositor

   Hiroyuki Hioki

   Article

   Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMSCVpuro-tdTomato

   Plasmid

   #97079

   Purpose

   Retroviral plasmid containing tdTomato for labeling of cells

   Depositor

   Lee Grimes

   Article

   Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

   Insert

   tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. m168

   Plasmid

   #34886

   Depositor

   Irvin Chen

   Article

   Morizono et al Nat Med. 2005 Mar;11(3):346-52. Epub 200

   Insert

   Sindbis virus envelope protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. cpLID-micro

   Plasmid

   #168995

   Purpose

   circularly permuted LOV2 based optical dimerization system (ssrA-cpLOV2 and sspB)

   Depositor

   Yubin Zhou

   Article

   He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

   Insert

   mCh-sspB-T2A-cpLID-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-mPCSK9

   Plasmid

   #122678

   Purpose

   Expresses mouse PCSK9 in mammalian cells

   Depositor

   Liming Pei

   Article

   Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

   Insert

   Mus musculus proprotein convertase subtilisin/kexin type 9 (Pcsk9) (Pcsk9 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. SIRT3 Flag

   Plasmid

   #13814

   Purpose

   Mammalian expression of human SIRT3 with flag tag

   Depositor

   Eric Verdin

   Article

   North et al Mol Cell. 2003 Feb . 11(2):437-44.

   Insert

   SIRT3 (SIRT3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. p6sheLLr

   Plasmid

   #37318

   Depositor

   John Schiller

   Article

   Pastrana et al Virology. 2005 Jul 5;337(2):365-72.

   Insert

   HPV6L1+L2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pACAGW-H2B-PAGFP-AAV

   Plasmid

   #33000

   Depositor

   Massimo Scanziani

   Article

   Lien et al Front Neural Circuits. 2011;5:16. Epub 2

   Insert

   H2B-PAGFP (H2BC21 Aequorea victoria, Human)
   

   Use

   AAV; Adeno associated virus

   Tags

   PAGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. human MMP-9 promoter

   Plasmid

   #53434

   Purpose

   promoter activity assay

   Depositor

   Douglas Boyd, Etty Benveniste

   Article

   Ma et al J Immunol. 2001 Nov 1;167(9):5150-9.

   Insert

   MMP-9 promoter (MMP9 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   MMP9

   Available since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Galphai2-mTurquoise2

    Plasmid

    #69621

    Purpose

    The heterotrimeric G-protein subunit, Gαi isoform 2, tagged with mTurquoise2

    Depositor

    Dorus Gadella

    Article

    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

    Insert

    Galphai2-mTurquoise2
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pM2s2AsG

    Plasmid

    #137924

    Purpose

    E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Insert

    Superfolder GFP
    

    Use

    Synthetic Biology; For use with e. coli nissle in…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mEos3.2-TOMM20-N-10

    Plasmid

    #57483

    Purpose

    Localization: Mitochondrial Membrane, Excitation: 507 / 572, Emission: 516 / 580

    Depositor

    Michael Davidson

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pUC57-gCarvi-T2A

    Plasmid

    #190333

    Purpose

    Fluorescent reporter for cAMP

    Depositor

    Naoto Saitoh

    Article

    Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

    Insert

    gCarvi
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. gCH27 (crCD55-4)

    Plasmid

    #217343

    Purpose

    crRNA targeting CD55

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    crCD55-4 (CD55 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. nos-Cas9.874Z1

    Plasmid

    #112685

    Purpose

    Express hSpCas9 under nanos promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Use

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    nanos

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Plasmid L5 attB::Pleft* mScarlet

    Plasmid

    #169410

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mScarlet

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    pLeft
    mScarlet proper
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LCV2 IRF3 KO

    Plasmid

    #217446

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human IRF3

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    sgRNA targeting IRF3 (IRF3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CD40L-SrtA

    Plasmid

    #121167

    Purpose

    pMP71 vector expressing Tomato - P2A - CD40L - SrtA - flag

    Depositor

    Gabriel Victora

    Article

    Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

    Insert

    CD40L-SrtA
    

    Use

    Retroviral

    Tags

    Tomato and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EF1a-EGFP (no signal control)

    Plasmid

    #170167

    Purpose

    Mammalian Expression of no signal control

    Depositor

    Christopher Moxham

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3b-8XGliBS:EGFP

    Plasmid

    #84602

    Purpose

    Gli protein-dependent EGFP reporter for analysis of Hedgehog signaling

    Depositor

    James Chen

    Article

    Hyman et al Proc Natl Acad Sci U S A. 2009 Aug 18;10

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    8X Gli-binding site

    Available since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBABE-puro-DN-hTERT

    Plasmid

    #1775

    Depositor

    Bob Weinberg

    Article

    Hahn et al Nat Med 1999 Oct;5(10):1164-70.

    Insert

    hTERT (TERT Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Dominant negative, 2 base pair mutation, catalyti…

    Promoter

    Available since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti.PGK.LifeAct-Ruby.W

    Plasmid

    #51009

    Purpose

    Lentivector that expresses LifeAct-Ruby from internal human PGK promoter

    Depositor

    Rusty Lansford

    Insert

    LifeAct-mRFPruby
    

    Use

    Lentiviral

    Tags

    LifeAct

    Expression

    Mammalian

    Mutation

    Promoter

    human PGK

    Available since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GFP-Tog

    Plasmid

    #29480

    Depositor

    Linda Wordeman

    Article

    Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

    Insert

    ch-TOG (CKAP5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K19R, E118G, G254R, K1834R

    Promoter

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDB5319

    Plasmid

    #204829

    Purpose

    An integrating plasmid containing both the inducible enotetSW1 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

    Depositor

    Li-Lin Du

    Article

    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

    Inserts

    tetR
    mECitrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CMV promoter and enotetSW1 promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mPA-GFP-CD81-10

    Plasmid

    #57124

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    CD81 (CD81 Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

26. Dynamitin-GFP

    Plasmid

    #51419

    Purpose

    expression of GFP-tagged wild-type dynamitin p50 in mammalian cells

    Depositor

    Trina Schroer

    Article

    Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

    Insert

    Dynamitin (DCTN2 Chicken)
    

    Use

    Tags

    GFP and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pT2K-CAGGS-T2TP

    Plasmid

    #114725

    Purpose

    expression in mammalian cells

    Depositor

    Martine Roussel

    Article

    Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

    Insert

    Tol2 transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CAGGS-MCS-NLS-3XFLAG-V5

    Plasmid

    #196383

    Purpose

    Empty backbone vector to insert cDNA for strong expression in mammalian cells

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Type

    Empty backbone

    Use

    Tags

    3xFLAG and V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAGGs

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJet1.2 ermB cassette

    Plasmid

    #117121

    Purpose

    Erythromycin resistance cassette

    Depositor

    Stephan Gruber

    Article

    Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

    Insert

    Erythromycin resistance cassette for Bacillus subtilis selection
    

    Use

    Golden gate donor vector for gene targeting in ba…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJT258 Lenti pEF-dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

    Plasmid

    #187334

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Mito-SNAP

    Plasmid

    #206480

    Purpose

    Encodes a mitochondrially-targeted SNAP tag for mammalian cell expression

    Depositor

    Erika Holzbaur

    Article

    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

    Insert

    Mitochondrial targeting signal from COX8A fused to SNAP (derived from human MGMT)
    

    Use

    Tags

    SNAPf

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PA-mCherry1-C1

    Plasmid

    #54495

    Purpose

    Localization: C1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    PA-mCherry1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. ER-moxMaple3

    Plasmid

    #120885

    Purpose

    mammalian expression of ER localized moxMaple3

    Depositor

    Erik Snapp

    Article

    Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

    Insert

    moxMaple3
    

    Use

    Tags

    KDEL ER retention sequence and bovine prolactin s…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLenti UBC LysoFlux (HaloTag7-mGFP-Galectin3)

    Plasmid

    #215008

    Purpose

    Express a Lysophagy reporter in mammalian cells (untested)

    Depositor

    Wade Harper

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Lentiviral

    Tags

    HaloTag(7)-mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PS-CFP2-Tubulin-6

    Plasmid

    #57251

    Purpose

    Localization: Microtubules, Excitation: 400 / 490, Emission: 468 / 511

    Depositor

    Michael Davidson

    Insert

    NM_006082.2 (TUBA1B Human)
    

    Use

    Tags

    PS-CFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

36. plRL2

    Plasmid

    #163631

    Purpose

    Optimized M. tuberculosis CRISPRi plasmid; L5 integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet-On

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLenti-nSpCas9-NoABE

    Plasmid

    #209043

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pME Integrin beta 1b

    Plasmid

    #105976

    Purpose

    entry clone for gateway cloning, Full length Integrin beta 1b without stop

    Depositor

    Caren Norden

    Article

    Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

    Insert

    Integrinbeta1b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pK170.AAV-TRE-Cre-WPRE (Supernova)

    Plasmid

    #85040

    Purpose

    For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    nlsCre-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pENTR-PIP-FUCCI

    Plasmid

    #118621

    Purpose

    Gateway cloning vector for the cell cycle indicator PIP-FUCCI

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-FUCCI
    

    Use

    Gateway cloning

    Tags

    mCherry and mVenus

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge