We narrowed to 17,203 results for: puro

1. pPB-cT3G-cERP2-CTCFL

   Plasmid

   #222546

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of CTCFL

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   CTCFL (CTCFL Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPB-cT3G-cERP2-3xFLAG-SMAD1-active

   Plasmid

   #222579

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of 3xFLAG-SMAD1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   SMAD1 (SMAD1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   S487E, S489E

   Promoter

   Available since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPB-cT3G-cERP2-3xFLAG-SMAD9-active

   Plasmid

   #222580

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of 3xFLAG-SMAD9

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   SMAD9 (SMAD9 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   S489E, S491E

   Promoter

   Available since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLX-EF1a_NKX2-1_W208L

   Plasmid

   #221486

   Purpose

   NKX2-1 W208L DNA binding mutation lentiviral overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   W208L

   Promoter

   EF1a

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLX-UBC_NKX2-1

   Plasmid

   #221495

   Purpose

   NKX2-1 short isoform lentiviral overexpression using UBC promoter

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLX-EF1a_NKX2-1_V205F

   Plasmid

   #221485

   Purpose

   NKX2-1 V205F DNA binding mutation lentiviral overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   V205F

   Promoter

   EF1a

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLX-SFFV_NKX2-1

   Plasmid

   #221496

   Purpose

   NKX2-1 short isoform lentiviral overexpression using SFFV promoter

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. sgRNA TR KO 2

   Plasmid

   #207610

   Purpose

   sgRNA 2 to knockout TR by replacement with a PuroR cassette

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   TCAGGCCGCAGGAAGAGGAA
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. sgRNA TR KO 1

   Plasmid

   #207609

   Purpose

   sgRNA 1 to knockout TR by replacement with a PuroR cassette

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   ACCCTAACTGAGAAGGGCGT
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p663-UBC-miniTurbo-V5-PRPF4B_IDG-K

    Plasmid

    #214463

    Purpose

    Gateway destination clone of PRPF4B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-PRPF4B (PRPF4B Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p663-UBC-miniTurbo-V5-STK19_IDG-K

    Plasmid

    #214465

    Purpose

    Gateway destination clone of STK19 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-STK19 (STK19 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX459-canis_PTGS2-4

    Plasmid

    #184730

    Purpose

    COX2-KO in MDCK

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    A gRNA targeting the dog COX2 gene.
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX459-homo_PTGS1-1

    Plasmid

    #184731

    Purpose

    COX1-KO in HeLa

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    A gRNA targeting the human COX1 gene.
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX459-homo_PTGS1-2

    Plasmid

    #184732

    Purpose

    COX1-KO in HeLa

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    A gRNA targeting the human COX1 gene.
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX459-homo_PTGS2-3

    Plasmid

    #184733

    Purpose

    COX2-KO in HeLa

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    A gRNA targeting the human COX2 gene.
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX459-homo_PTGS2-4

    Plasmid

    #184734

    Purpose

    COX2-KO in HeLa

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    A gRNA targeting the human COX2 gene.
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX459-homo_PLA2G4A-1

    Plasmid

    #184735

    Purpose

    cPLA2-KO in HeLa

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    A gRNA targeting the human cPLA2 gene.
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX459-homo_PLA2G4A-2

    Plasmid

    #184736

    Purpose

    cPLA2-KO in HeLa

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    A gRNA targeting the human cPLA2 gene.
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX459-canis_PTGS1-3

    Plasmid

    #184727

    Purpose

    COX1-KO in MDCK

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    A gRNA targeting the dog COX1 gene.
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX459-canis_PTGS1-4

    Plasmid

    #184728

    Purpose

    COX1-KO in MDCK

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    A gRNA targeting the dog COX1 gene.
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pX459-canis_PTGS2-3

    Plasmid

    #184729

    Purpose

    COX2-KO in MDCK

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    A gRNA targeting the dog COX2 gene.
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLentiCRISPR-v2 sgUCKL1

    Plasmid

    #211523

    Purpose

    Deletes UCKL1

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    sgUCKL1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLentiCRISPR-v2 sgUCK2

    Plasmid

    #211522

    Purpose

    Deletes UCK2

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    sgUCK2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLentiCRISPR-v2 sgUGP2

    Plasmid

    #211521

    Purpose

    Deletes UGP2

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    sgUGP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLentiCRISPR-v2 sgBAK1

    Plasmid

    #211520

    Purpose

    Deletes BAK1

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    sgBAK1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLentiCRISPR-v2 sgCASP8

    Plasmid

    #211519

    Purpose

    Deletes CASP8

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    sgCASP8
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pACBE-ASR (pXK-607)

    Plasmid

    #208822

    Purpose

    Universal Antibiotic Screening Reporter for adenine and cytosine base editors. Key Construct: CMV-PuroR*(ATAACG)-eGFP-hU6-gRNA.

    Depositor

    Kun Xu

    Article

    Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi

    Type

    Empty backbone

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSCALPS-EGFP-Cre

    Plasmid

    #207132

    Purpose

    Lentiviral vector to express EGFP-Cre

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    EGFP-Cre
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SSFV

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMXSIP-Rab5B-HA

    Plasmid

    #204709

    Purpose

    expresses Rab5B-HA in mammalian cells

    Depositor

    William Kaelin

    Article

    Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

    Insert

    RAB5B (RAB5B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMXSIP-TMEM141-FLAG

    Plasmid

    #204702

    Purpose

    expresses TMEM141-FLAG in mammalian cells

    Depositor

    William Kaelin

    Article

    Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

    Insert

    TMEM141 (TMEM141 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. XLone-mARG2

    Plasmid

    #173792

    Purpose

    Mammalian acoustic reporter gene 1 cassette 2 on XLone plasmid

    Depositor

    Xiaojun Lian

    Article

    Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

    Insert

    GvpN, F G L S K J U
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G promoter

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. SP_H6_Halo_WT_KDEL_pBABEpu

    Plasmid

    #183688

    Purpose

    Retroviral expression of ER targeted H6-tagged Halotag Wild type

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Insert

    ER Halo
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p663-UBC-miniTurbo-V5-MAST3_IDG-K

    Plasmid

    #198007

    Purpose

    Gateway destination clone of MAST3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-MAST3 (MAST3 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p667-UBC-ULK4-V5-miniTurbo-STOP_IDG-K

    Plasmid

    #198011

    Purpose

    Gateway destination clone of ULK4 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    ULK4-V5-miniTurbo (ULK4 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pXPR_003_sgTFAP4 guide 1

    Plasmid

    #193450

    Purpose

    ko TFAP4 guide 1

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    Tfap4
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p663-UBC-miniTurbo-V5-CDKL3_IDG-K

    Plasmid

    #158177

    Purpose

    Gateway destination clone of CDKL3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDKL3 (CDKL3 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p667-UBC-TAOK2-V5-miniTurbo_IDG-K

    Plasmid

    #189888

    Purpose

    Gateway destination clone of TAOK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TAOK2 (TAOK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. InterTag

    Plasmid

    #186910

    Purpose

    Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR and the other side expressing BFP and puromycin resistance gene

    Depositor

    Allen Liu, Taekjip Ha

    Article

    Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

    Insert

    SpyTag003-sfCherry11-PDGFR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p667-UBC-PNCK-V5-miniTurbo_IDG-K

    Plasmid

    #189897

    Purpose

    Gateway destination clone of PNCK (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    PNCK (PNCK Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p667-UBC-TSSK4-V5-miniTurbo_IDG-K

    Plasmid

    #189901

    Purpose

    Gateway destination clone of TSSK4 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TSSK4 (TSSK4 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge