We narrowed to 17,996 results for: puro

Showing: 4841 - 4860 of 17996 results

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pHPHS853

Plasmid

#228236

Purpose

Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible EYFP reporter for integration into Bxb1 attP_GA landing pad

Depositor

Arvind Rasi Subramaniam

Article

Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

Inserts

mCherry
EYFP

Tags

HA and PuroR

Expression

Mammalian

Promoter

TRE3GV and none

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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px459-TDP 43 sgRNA2

Plasmid

#133763

Purpose

Expresses Cas9 and human TDP-43 sgRNA

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-NLS-CBRed-d2-hygro

Plasmid

#139196

Purpose

non-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puro

Depositor

Matthew Lazzara

Article

Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

Insert

CBRed

Use

Luciferase and Retroviral

Tags

d2 PEST domain and nuclear localization signal (N…

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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PX459-TP53-exon4

Plasmid

#217455

Purpose

For TP53 knockout targeting exon 4 of TP53

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CRISPR Cas9 guide for TP53 (TP53 Human)

Use

CRISPR

Expression

Mammalian

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti CMV-IFP2.0 T2A HO1

Plasmid

#59427

Purpose

Express infrared fluorescent protein IFP2.0 plus HO1 enzyme

Depositor

Xiaokun Shu

Article

Yu et al Nat Commun. 2014 May 15;5:3626. doi: 10.

Insert

IFP2.0T2AHO1 IRES EGFP

Use

Lentiviral

Promoter

CMV

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pCPmacroH2A.1-GS3

Plasmid

#204759

Purpose

Expression of Cas9 and human macroH2A.1

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

macroH2A1 (MACROH2A1 Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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LEPZ

Plasmid

#111161

Purpose

miR-E (miR-30 variant)-based RNAi with ZsGreen reporter

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Retroviral

Mutation

WT

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

Plasmid

#222993

Purpose

Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Article

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Insert

HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Promoter

pCAG

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1 p18 shRNA

Plasmid

#26631

Depositor

David Sabatini

Article

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

p18 shRNA (LAMTOR1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pMCB619

Plasmid

#171011

Purpose

CRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites).

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

U6:sgRNA_Puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pscALPS-HIV-1 Group P-vpr

Plasmid

#115837

Purpose

Encodes codon optimized 3xFLAG-HIV1 Group P Vpr (HQ179987) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HIV1 Group P Vpr (HQ179987)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS-N-HA

Plasmid

#130913

Purpose

Expresses human cGAS N terminus(aa1-159)-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGAS N terminus

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

Plasmid

#222992

Purpose

Mammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Article

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Insert

HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Promoter

pCAG

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_S139A-GS3

Plasmid

#204749

Purpose

Expression of Cas9 and human H2AX with the S139A mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX S139A (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the S139A mutation

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-DNAJC5

Plasmid

#205727

Purpose

Mammalian expression of DNAJC5

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Lentiviral

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBMN-AS-YY1

Plasmid

#154943

Purpose

Knocking down of human YY1 through shRNA and amiRNA targeting YY1

Depositor

Claes Wadelius

Article

Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

Insert

shRNA and amiRNA against YY1 (YY1 Synthetic, Human)

Use

Lentiviral

Expression

Mammalian

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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Barcode backbone

Plasmid

#158665

Purpose

This plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combination

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Type

Empty backbone

Use

Lentiviral

Tags

hcRED

Expression

Mammalian

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pscALPS-HIV-1 Group O-vpr

Plasmid

#115836

Purpose

Encodes codon optimized 3xFLAG-HIV1 Group O Vpr (L20571) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HIV1 Group O Vpr (L20571)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pJPT018-LAT_WT-HaloTag-3x FLAG

Plasmid

#202502

Purpose

Express WT-LAT

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

LAT (LAT Human)

Tags

HaloTag-3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe-FLAG-Neuroserpin

Plasmid

#58261

Purpose

Retroviral vector for expressing Neuroserpin in mammalian cells

Depositor

Joan Massague

Article

Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

Insert

Neuroserpin (SERPINI1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only