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pRK5 GST MetaP2

Plasmid

#112750

Purpose

express HA-GST-FRB-MetaP2

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

HA-GST-FRB-MetaP2 (METAP2 Human)

Tags

GST

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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sgMAPKAP1_2

Plasmid

#124911

Purpose

Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

CRISPR and Lentiviral

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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sgMAPKAP1_3

Plasmid

#124913

Purpose

Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

CRISPR and Lentiviral

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF138-delta 86-105

Plasmid

#78922

Purpose

Mammalian expression of RNF138 with delta 86-105 and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Tags

EGFP

Expression

Mammalian

Mutation

delta 86-105

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-RNF138-delta 158-180

Plasmid

#78923

Purpose

Mammalian expression of RNF138 with delta 158-180 and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Tags

EGFP

Expression

Mammalian

Mutation

delta 159-180

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-RNF138-delta 189-215

Plasmid

#78924

Purpose

Mammalian expression of RNF138 with delta 189-215 and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Tags

EGFP

Expression

Mammalian

Mutation

delta 189-215

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pCGT-HCF-1-C600

Plasmid

#99364

Purpose

C-term of HCFC1 protein

Depositor

Winship Herr

Article

Wilson et al Mol Cell Biol. 2000 Sep;20(18):6721-30.

Insert

HCF-1 C-term 1436-2035 (HCFC1 Human)

Tags

2x tandem T7 tag and C-myc

Expression

Mammalian

Mutation

Deletion of HCFC1 cDNA

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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mCardinal-Alpha-Actinin-19

Plasmid

#56152

Purpose

Localization: Focal Adhesions / Actin, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Alpha-Actinin (ACTN1 Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
GST-SF3B2 wild type aa 401-550 fragment

Plasmid

#67615

Purpose

bacterial expression of wild type GST-SF3B2 fragment (401-550)

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

splicing factor 3b subunit 2 (SF3B2 Human)

Tags

GST

Expression

Bacterial

Mutation

fragment of amino acids 401-550

Promoter

tac

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS

Plasmid

#59388

Purpose

Destination vector with RBPMS for stable cell line generation

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pMTTH+mEGFP-LANA-H1.4CTD

Plasmid

#157802

Purpose

Expression of mEGFP, LANA peptide, and human linker histone H1.4 CTD fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-LANA-H1.4CTD (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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CRAF_S29A_pcDNA3.1

Plasmid

#86506

Purpose

Expresses CRAF_S29A in mammalian cells [Edit]

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

CRAF (RAF1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S29A

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23 T230A 2014 EBOV + Mucin-Like Domain

Plasmid

#86017

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Changed threonine 230 to alanine

Promoter

CMV IE

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23 D637G 2014 EBOV + Mucin-Like Domain

Plasmid

#86018

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Changed aspartic acid 637 to glycine

Promoter

CMV IE

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p4948 pcDNA4c His Xpress SV40NLS hBrd4 CTD

Plasmid

#14442

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 CTD (BRD4 Human)

Tags

SV40 NLS

Expression

Mammalian

Mutation

amino acids 1047-1362 of Brd4 (the CTD). Function…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pADSL-2XStrep

Plasmid

#125684

Purpose

expresses human ADSL protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

ADSL (ADSL Human)

Tags

2X Strep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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B52 + TIMELESS sgSTOP

Plasmid

#100713

Purpose

B52 plasmid expressing TIMELESS sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting TIMELESS (cloned using BbsI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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B52 + PARP4 sgSTOP

Plasmid

#100711

Purpose

B52 plasmid expressing PARP4 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting PARP4 (cloned using BbsI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pfast bac cry1myc

Plasmid

#51892

Purpose

Backbone plasmid is pfastBacHTa from invitrogen

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

cry1 (Cry1 Mouse)

Tags

3x Flag and myc

Promoter

polyhedrin promoter

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
Opto-GPR75

Plasmid

#106032

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR75; Opto-GPR75; C10)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR75 (GPR75 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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