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Showing: 9721 - 9760 of 157662 results

  5. pET22b_PstS_1

    Plasmid
    #78198
    Purpose
    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Article
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A197C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A197C (please see depositors comment below)
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78198-plasmid-map-sequence-id-146048

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  7. pFastBac1-His10TEV

    Plasmid
    #159427
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Use
    Tags
    TEV-protease-cleavable His10-tag
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    159427-plasmid-map-sequence-id-333995

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  10. pLentiGuide-BlastR

    Plasmid
    #196970
    Purpose
    Lentiviral acceptor vector for pegRNAs with blasticidin resistance
    Depositor
    Leopold Parts
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    196970-plasmid-map-sequence-id-441210

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  11. pCB578

    Plasmid
    #141095
    Purpose
    E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array
    Depositor
    Chase Beisel
    Article
    Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d
    Inserts
    Cas9 and tracrRNA
    repeat-spacer-repeat array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    141095-plasmid-map-sequence-id-292312

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  14. pScarlessHD-DsRed

    Plasmid
    #64703
    Purpose
    Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    piggyBac-3xP3-DsRed
    Use
    Tags
    Expression
    Mutation
    Promoter
    3xP3
    Available since
    Dec. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    64703-plasmid-map-sequence-id-443894

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  28. mEmerald-N1

    Plasmid
    #53976
    Purpose
    Localization: N1 Cloning Vector, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    53976-plasmid-map-sequence-id-212898

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  29. pTP1112

    Plasmid
    #104158
    Purpose
    Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)
    Use
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104158-plasmid-map-sequence-id-200058

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  30. pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40

    Plasmid
    #100054
    Purpose
    AAV expression of humanized ChR2 with H134R mutation fused to mCherry driven by CAG promoter for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV1, AAV2, AAV5, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    H134R mutation in ChR2
    Promoter
    CAG
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100054-plasmid-map-sequence-id-183171

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  33. PL-SIN-EOS-S(4+)-EiP

    Plasmid
    #21314
    Purpose
    Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP and Puro resistance
    Depositor
    James Ellis
    Article
    Hotta et al Nat Methods. 2009 May . 6(5):370-6.
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EOS-S(4+) promoter region
    Available since
    June 1, 2009
    Availability
    Academic Institutions and Nonprofits only
    21314-plasmid-map-sequence-id-305770

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  35. iRFP-H2A

    Plasmid
    #158691
    Purpose
    This plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markers
    Depositor
    Ravid Straussman
    Article
    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:
    Insert
    iRFP670
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    158691-plasmid-map-sequence-id-311047

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  36. gCBEv2

    Plasmid
    #220617
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv2) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    hUNG2-nSpCas9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220617-plasmid-map-sequence-id-436955

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  39. pLKO.1 mCherry

    Plasmid
    #128073
    Purpose
    puro exchanged to mCherry
    Depositor
    Oskar Laur
    Insert
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    no
    Promoter
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128073-plasmid-map-sequence-id-255273

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Showing: 9721 - 9760 of 157662 results