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161,838 results

Showing: 9721 - 9760 of 161838 results

  2. pCI_FLAG-GFP(Y39TAG)-6His

    Plasmid
    #223507
    Purpose
    Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39
    Depositor
    Edward Lemke
    Insert
    GFP(Y39TAG)
    Use
    Tags
    6xHis and FLAG
    Expression
    Mammalian
    Mutation
    Y39TAG
    Promoter
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223507-plasmid-map-sequence-id-447435

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  3. pdRBFC-NS1-YN

    Plasmid
    #74723
    Purpose
    Express C-terminal YN fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labeling
    Depositor
    Aiming Wang
    Article
    Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1
    Insert
    Influenza A virus NS1 protein, partial
    Use
    Double-stranded rna labeling
    Tags
    HA and YFP N-terminal half domain
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    May 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    74723-plasmid-map-sequence-id-146367

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  7. pX551-miniCMV-SpCas9

    Plasmid
    #107031
    Purpose
    AAV plasmid expressing Cas9 under control of miniCMV promoter
    Depositor
    Alex Hewitt
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107031-plasmid-map-sequence-id-208545

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  12. LentiGuide Cherry

    Plasmid
    #170510
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Pavel Tolar
    Article
    Newman et al biorXiv 2021.03.02.433398
    Inserts
    S. pyogenes sgRNA cassette
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a and hU6
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170510-plasmid-map-sequence-id-343694

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  17. pLVX-TetOne-Puro-EcSTH-FLAG

    Plasmid
    #218628
    Purpose
    This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.
    Depositor
    Valentin Cracan
    Article
    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038
    Insert
    EcSTH
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218628-plasmid-map-sequence-id-432188

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  24. pKAR2-Br512g3.1

    Plasmid
    #171200
    Purpose
    Expresses Br512g3.1 polymerase in E.coli
    Depositor
    Andrew Ellington
    Article
    Bhadra et al medRxiv 2022.08.09.22278605
    Insert
    Engineered Bst DNA Polymerase I Large Fragment
    Use
    Tags
    8x His tag; Villin Headpiece 47 amino acid (vHP47…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    171200-plasmid-map-sequence-id-339557

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  26. FUGW-APOE-E3-T2A-mCherry

    Plasmid
    #190808
    Purpose
    Express APOE E3 allele from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    E3
    Promoter
    Ubi C
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    190808-plasmid-map-sequence-id-372853

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  34. MYD88

    Plasmid
    #13092
    Depositor
    Ruslan Medzhitov
    Insert
    MYD88 (Myd88 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13092-plasmid-map-sequence-id-210991

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  37. TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

    Plasmid
    #216152
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic exon and C-terminal FLAG
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216152-plasmid-map-sequence-id-437762

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  38. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60226
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60226-plasmid-map-sequence-id-176980

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  40. pJRH029

    Plasmid
    #171060
    Purpose
    Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Insert
    Gag-spyCas9
    Use
    CRISPR and Lentiviral
    Tags
    2x SV40 NLS and 3x FLAG tag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171060-plasmid-map-sequence-id-457342

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Showing: 9721 - 9760 of 161838 results