165,025 results

1. Klc1-GFP

   Plasmid

   #186617

   Purpose

   Encodes Kinesin Light Chain 1 fused to GFP

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Kinesin Light Chain 1 (KLC1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_CreNLS_PP7

   Plasmid

   #232439

   Purpose

   expression plasmid to produce Cre-NLS mRNA with a PP7 aptamer in the 3' UTR

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   Cre–MYC-NLS
   

   Expression

   Mammalian

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. SPHK2 gRNA (BRDN0001148465)

   Plasmid

   #77093

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK2 (SPHK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a-Cas9-2XNES-2ERT2

   Plasmid

   #120551

   Purpose

   Express Cas9, 2xNES and 2 tandem ERT2 in mammalian cells

   Depositor

   Yu Wang

   Article

   Zhao et al Mol Ther Nucleic Acids. 2018 Dec 7;13:20

   Insert

   Cas9, NES and ERT2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FLAG-ATBF1

   Plasmid

   #40927

   Depositor

   Jin-Tang Dong

   Article

   Dong et al J Biol Chem. 2010 Oct 22;285(43):32801-9

   Insert

   ATBF1 (ZFHX3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   See depositor comments below.

   Promoter

   CMV

   Available Since

   March 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR

   Plasmid

   #210525

   Purpose

   AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   mtagBFP-KASH
   U6-gRNA
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. VPS13A^mCherry

   Plasmid

   #118758

   Purpose

   VPS13A internally tagged with mCherry at residue 1372.

   Depositor

   Pietro De Camilli

   Article

   Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

   Insert

   VPS13A (VPS13A Human)
   

   Tags

   mCherry inserted at position 1372

   Expression

   Mammalian

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57-LbNOX

   Plasmid

   #75285

   Purpose

   This plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

   Insert

   LbNOX
   

   Tags

   C-terminal FLAG tag with linker

   Mutation

   Human codon optimized

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-GRP78/BiP

   Plasmid

   #32701

   Depositor

   Richard C. Austin

   Article

   Werstuck et al J Clin Invest. 2001 May;107(10):1263-73.

   Insert

   GRP78/BiP (HSPA5 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-(empty)-TAG

    Plasmid

    #138209

    Purpose

    To facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' region

    Depositor

    Neal Devaraj

    Article

    Busby et al Methods Enzymol. 2020;641:373-399. doi:

    Type

    Empty backbone

    Use

    T7 transcription

    Tags

    TAG sequence

    Expression

    Mammalian

    Promoter

    CMV Promoter, T7 Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti_PEmax-2A-MLH1dn-2A-BSD

    Plasmid

    #234072

    Purpose

    For lentiviral expression of PEmax with MLH1dn and blasticidin resistance

    Depositor

    Greg Findlay

    Article

    Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

    Insert

    PEmax-2A-MLH1dn
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF-1α core promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO_TOMM20-SNAP-tag2-T2A-EGFP

    Plasmid

    #226516

    Purpose

    Mamalian cell expression of SNAP-tag2 on mitochondrial surface and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    TOMM20-SNAP-tag2-T2A-EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCT5-bac1.8

    Plasmid

    #119871

    Purpose

    Constitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

    Depositor

    Claudia Schmidt-dannert

    Article

    Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

    Inserts

    CymR'
    sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    SNP in 592 bp

    Promoter

    PserA promoter and Pveg promoter with CuO sequence

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOSIP-KO (KanR, 186)

    Plasmid

    #45985

    Depositor

    Drew Endy, Keith Shearwin

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3/mStayGold(c4)=UtrCH

    Plasmid

    #212020

    Purpose

    For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    UtrCH
    

    Tags

    mStayGold(c4)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Cas12i2-T2A-EGFP

    Plasmid

    #188270

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    Cas12i2
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-EF1A-CD19.28H.28TM.BB.3z

    Plasmid

    #200679

    Purpose

    Modular CD28hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD28HD-CD28TM-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Promoter

    EF1a-short

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-REPORT (PGK) 12xHRE-NanoLuc

    Plasmid

    #208575

    Purpose

    hypoxic response element driven NanoLuc Lentiviral reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    NanoLuciferase
    d2eGFP
    12xHRE
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    3x SV40 NLS and PEST

    Expression

    Mammalian

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti CMV RasV12 Neo (w108-1)

    Plasmid

    #22259

    Depositor

    Eric Campeau

    Insert

    HRAS G12V (HRAS Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    G12V mutant

    Available Since

    Jan. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. htrpC6-YFP

    Plasmid

    #21084

    Depositor

    Craig Montell

    Article

    Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

    Insert

    TrpC6 (TRPC6 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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