We narrowed to 12,952 results for: sequence
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Plasmid#186396PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of a regulatory sequence cloned in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only
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pENT-L1-Kozak-Venus-stop-L2
Plasmid#186360PurposeEntry clone with ORF encoding Venus fluorescent protein flanked by Gateway recombination sequencesDepositorInsertVenus yellow fluorescent protein
UseExpression of the venus fluorescent proteinTagsExpressionMutationPromoterAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-eGFP-Pep-H
Plasmid#185788PurposeEncodes enhanced green flourescent protein linked to Pep-H self-assembling peptide via a GS-linker sequenceDepositorInserteGFP-Pep4
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A TMD Y616M
Plasmid#140454PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and Y616M mutantDepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, tyrosine 616 to methioni…PromoterAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A TMD L624N
Plasmid#140455PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and L624N mutantDepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, leucine 624 to asparaginePromoterAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A TMD A634N
Plasmid#140456PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and A634N mutantDepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, alanine 634 to asparaginePromoterAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4_HLA-flag-GRM3 C127A-VN
Plasmid#140449PurposeMammalian expression plasmid for FLAG-tagged mGluR3 fused to the N-terminal half of split fluorescent Venus (VN)DepositorInsertGRM3 (GRM3 Human)
UseTagsFLAG tag, Signal/leader sequence from HLA class I…ExpressionMammalianMutationCysteine 127 to alaninePromoterAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A TMD I578A
Plasmid#140452PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and I578A mutantDepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, isoluecine 578 to alaninePromoterAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A TMD F588V
Plasmid#140453PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and F588V mutantDepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, phenylalanine 588 to val…PromoterAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1023-Tier1-PhCMV-p2A-PuroR
Plasmid#169497PurposeTier-1 vector encoding PhCMV-driven p2A sequence fused to a marker for puromycine selection (PhCMV-p2A-PuroR-pA).DepositorInsertPCMV-driven P2A-PuroR
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4E-T-L36AL39A_T
Plasmid#147604PurposeInsect Expression of Dm4E-T-L36AL39ADepositorInsertDm4E-T-L36AL39A (4E-T Fly)
UseTagsExpressionInsectMutationTwo non silent mutations (H628R and N659S) compar…PromoterAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-10xmut_G
Plasmid#146428PurposeInsect Expression of DmGW182-10xmutDepositorInsertDmGW182-10xmut (gw Fly)
UseTagsExpressionInsectMutationMultiple mutations compared to NM_166780.1, see a…PromoterAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat-dsRNAres_J
Plasmid#146692PurposeInsect Expression of DmHPat-dsRNAresDepositorInsertDmHPat-dsRNAres (Patr-1 Fly)
UseTagsExpressionInsectMutationtwo non silent, A479V, G568D and one silent mutat…PromoterAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-HsAGO2_E
Plasmid#146224PurposeInsect Expression of HsAGO2DepositorInsertHsAGO2 (AGO2 Human)
UseTagsExpressionInsectMutationContains a K660E mutation compared to the sequenc…PromoterAvailable SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg5-G120E_W
Plasmid#147819PurposeMammalian Expression of HsSmg5-G120EDepositorInsertHsSmg5-G120E (SMG5 Human)
UseTagsExpressionMammalianMutationone silent mutation compared to the sequence give…PromoterAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg7_633-1091_T
Plasmid#147558PurposeMammalian Expression of HsSmg7_633-1091DepositorInsertHsSmg7_633-1091 (SMG7 Human)
UseTagsExpressionMammalianMutationone non silent mutation V854I compared to the seq…PromoterAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_SUS1 5'ss_No_BP_pBP-pUC18
Plasmid#100990PurposeContains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutationDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->…PromoterAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmHPat-del500-684-dsRNAres_H
Plasmid#146466PurposeInsect Expression of DmHPat-del500-684-dsRNAresDepositorInsertDmHPat-del500-684-dsRNAres (Patr-1 Fly)
UseTagsExpressionInsectMutationtwo silent mutations compared to the sequence gi…PromoterAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2-W366AW390A_P
Plasmid#147235PurposeInsect Expression of CeAIN2-W366AW390ADepositorInsertCeAIN2-W366AW390A
UseTagsExpressionInsectMutationDeletion of two AA SD 378-379 compared to the seq…PromoterAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHPat_57-499_B
Plasmid#145979PurposeInsect Expression of DmHPat_57-499DepositorInsertDmHPat_57-499 (Patr-1 Fly)
UseTagsExpressionInsectMutationtwo silent mutations compared to the sequence giv…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm1_A
Plasmid#145886PurposeInsect Expression of Dm-Lsm1DepositorInsertDm-Lsm1
UseTagsExpressionInsectMutationTwo silent mutation compared to the sequence give…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm4_A
Plasmid#145889PurposeInsect Expression of Dm-Lsm4DepositorInsertDm-Lsm4
UseTagsExpressionInsectMutationone non silent mutation G127R compared to the seq…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat_1-225_A
Plasmid#145870PurposeInsect Expression of DmHPat_1-225DepositorInsertDmHPat_1-225 (Patr-1 Fly)
UseTagsExpressionInsectMutationone non silent mutation N218K compared to the seq…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHPat_1-225_A
Plasmid#145875PurposeInsect Expression of DmHPat_1-225DepositorInsertDmHPat_1-225 (Patr-1 Fly)
UseTagsExpressionInsectMutationone non silent mutation N218K compared to the seq…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHPat_1-499_A
Plasmid#145876PurposeInsect Expression of DmHPat_1-499DepositorInsertDmHPat_1-499 (Patr-1 Fly)
UseTagsExpressionInsectMutationtwo silent mutations compared to the sequence giv…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmXRN1_A
Plasmid#145879PurposeInsect Expression of DmXRN1DepositorInsertDmXRN1 (pcm Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCRW2
Plasmid#127380PurposepBC with 4 repeats of 80bp DNA templateDepositorInsert4 repeats of 80 bp DNA template sequence
UseTagsExpressionBacterialMutationPromoterlac promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-MjcDOPA5GT
Plasmid#162530PurposeMirabilis jalapa cyclo-DOPA 5-O-glucosyltransferase coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.DepositorInsertcDOPA5GT
UsePuc19-derivedTagsExpressionBacterialMutationPromoterAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-NbPT5bPro
Plasmid#159416PurposeNicotiana benthamiana PT5b promoter sequence (1068 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.DepositorInsertNbPT5b Promoter
UsePuc19-derivedTagsExpressionBacterialMutationPromoterAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-NbBCP1bPro
Plasmid#159417PurposeNicotiana benthamiana BCP1b promoter sequence (1231 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.DepositorInsertNbBCP1b Pro
UsePuc19-derivedTagsExpressionBacterialMutationPromoterAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only