We narrowed to 13,669 results for: sequence
-
Plasmid#222421PurposeTo express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15)DepositorInsertMAPPER
TagsmScarletI3-6DG5ExpressionMammalianAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cytoplasmic PancACe
Plasmid#215708PurposeExpresses acetyl-CoA biosensor in the cytoplasmDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and cytoplasmic localization sequenceExpressionMammalianPromoterCMVAvailable SinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mitochondrial PancACe
Plasmid#215710PurposeExpresses acetyl-CoA biosensor in the mitochondriaDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and mitochondrial localization sequence…ExpressionMammalianPromoterCMVAvailable SinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
hprtSAGFP
Plasmid#41594PurposeIn vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequencesDepositorInsertSA-GFP reporter
UseMouse Targeting; Dsb repair reporter substrateExpressionMammalianAvailable SinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5x_UnaG
Plasmid#163125PurposeA plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.DepositorInsertUnaG
TagsHis and MBPExpressionBacterialAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5E-4xnrUAS
Plasmid#87120PurposeMultisite gateway entry clone containing 4 non-repetitive UAS (upstream activation sequence) elementsDepositorInsert4xnrUAS (non repetitive)
UseGateway vectorAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
mtsCOXVIIIx2-B-gTEMP/pcDNA3
Plasmid#199806PurposeFluorescent temperature indicator B-gTEMP fused with mitochondrial targeting sequenceDepositorInsertB-gTEMP
TagsmtsCOXVIIIx2ExpressionMammalianAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA BfuAI stuffer
Plasmid#50920PurposeU6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sitesDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mRuby3
Plasmid#155221PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
TagsmRuby3ExpressionMammalianAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-GPI
Plasmid#65294Purposesynchronize trafficking of EGFP-GPI from the ER (RUSH system)DepositorInsertGPI anchor
TagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianPromoterCMVAvailable SinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-SB-NLS
Plasmid#228870PurposePE7 connected to MLH1-SB-NLS via 2A sequenceDepositorInsertPE7-2A-MLH1-SB-NLS
UseCRISPRAvailable SinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1
Plasmid#20061PurposeBacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formationDepositorInsertMSP1D1
Tags7-His tagExpressionBacterialMutationdeletion mutant (1-11) of MSP1; His-tag on N-term…Available SinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
PKD1-F (pCI-PKD1-Flag)
Plasmid#21369DepositorInsertautosomal dominant polycystic kidney disease type I (PKD1 Human)
TagsFLAGExpressionMammalianMutationwild-type sequence*Available SinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
GLURP-bio-His
Plasmid#50802PurposeExpresses enzymatically monobiotinylated full length GLURPDepositorInsertGLURP
TagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable SinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
SERA5-bio-His
Plasmid#50817PurposeExpresses enzymatically monobiotinylated full length SERA5DepositorInsertSERA5
TagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable SinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
PFD1130w-bio-His
Plasmid#50823PurposeExpresses enzymatically monobiotinylated full length PFD1130wDepositorInsertPFD1130w
TagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable SinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
SERA4-bio-His
Plasmid#50816PurposeExpresses enzymatically monobiotinylated full length SERA4DepositorInsertSERA4
TagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable SinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cytoplasmic cpGFP
Plasmid#215709PurposeExpresses cpGFP in the cytoplasmDepositorInsertcpGFP
UseLentiviralTagsFLAG, HA, and cytoplasmic localization sequenceExpressionMammalianMutationcircularly permuted GFPPromoterCMVAvailable SinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-blast
Plasmid#26655Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiExpressionMammalianAvailable SinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-SUMO-MarathonRT
Plasmid#109029PurposeGroup II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”DepositorInsertERE_01490
Tags6xHis-SUMO tagExpressionBacterialPromoterT7Available SinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1362 scFv entry plasmid
Plasmid#201912PurposeEntry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)DepositorInsertStuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
ExpressionMammalianPromoterCAGAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
EKAREN5-gl
Plasmid#225958PurposeThe YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.DepositorInsertEKAREN5-gl
UseLentiviralTagsnls localization motifExpressionMammalianPromoterEF-1-alpha promoterAvailable SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHL-sec
Plasmid#99845PurposeMammalian expression with secretion signal sequenceDepositorAvailable SinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
ERM-APEX2
Plasmid#79055PurposeSoybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.DepositorInsertAPEX2 anchored to the cytosolic face of the ER membrane
UseLentiviralTagsResidues 1-27 of P450 oxidase 2C1 and V5ExpressionBacterial and MammalianPromoterCMV immearly promotorAvailable SinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NBid-PhoCl2c-CBid
Plasmid#164051PurposeOptogenetic manipulation of cell apoptosis using PhoCl2cDepositorInsertNBid-PhoCl2c-CBid
Tagskozak sequenceExpressionMammalianPromoterCMV promotorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-Ecadherin
Plasmid#65286Purposesynchronize trafficking of Ecadherin from the ER (RUSH system)DepositorInsertEcadherin
TagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianPromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
p416TEF1 Su9-Cystector (Cysteine biosensor)
Plasmid#250655PurposeMitochondrial matrix expression plasmid for Cystector (Cysteine biosensor) in Saccharomyces cerevisiaeDepositorInsertSu9-Cystector (Cysteine biosensor)
TagsMitochondrial targetting sequence based on Neuros…ExpressionYeastPromoterTEF1Available SinceMarch 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
APEX2-OMM
Plasmid#79056PurposeSoybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling proteinDepositorInsertSoybean APEX2
UseLentiviralTagsflag and last 31 amino acids of human MAVSExpressionBacterial and MammalianPromoterCMV immearly promotorAvailable SinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMA3790
Plasmid#70110PurposemtDNA elimination/production of rho-0 cellsDepositorInsertsEGFP
hUNG1 Y147A
Neo
TagsNone, human OTC mitochondrial targeting sequence,…ExpressionMammalianMutationY147A, aa's 1-77 removedPromoterCMV, EF1a, and SV40 and nativeAvailable SinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-DUX4-WT
Plasmid#99282PurposeAll-in-one lentivector with tet-inducible DUX4 (wildetype nucleotide sequence)DepositorAvailable SinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only