We narrowed to 10,761 results for: T7

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pET28a-HaloTag7-SnoopLigase

Plasmid

#105627

Purpose

Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

HaloTag7-SnoopLigase

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBroad-T7RNAP-apraR

Plasmid

#199993

Purpose

T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) apraA

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

T7RNAP Circuit

Expression

Bacterial

Mutation

copFnull

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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UbcH5A

Plasmid

#61081

Purpose

express a human E2

Depositor

Cynthia Wolberger

Article

Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

Insert

UbcH5A (UBE2D1 Human)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pET_sfCherry(1-10)_32aalinker_sfCherry(11)

Plasmid

#83030

Purpose

Bacterial expression vector for split sfCherry screening

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry(1-10)_32aalinker_sfCherry(11)

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pET-MCN-10His-StayGold

Plasmid

#211360

Purpose

Bacterial expression of the bright and photostable StayGold fluorescent protein. Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Article

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

StayGold

Tags

10xHis-tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Human PNP his-tagged

Plasmid

#64076

Purpose

Expression of human Purine Nucleoside Phosphorylase

Depositor

Vern Schramm

Article

Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8

Insert

Purine Nucleoside Phosphorylase (PNP Human)

Tags

Histidine tag

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pKL002

Plasmid

#98921

Purpose

pET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.

Depositor

Joel Kralj

Article

Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

Inserts

PROPS
GCaMP6f

Expression

Bacterial

Promoter

T7

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMJ826

Plasmid

#39316

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Histidine 840 to Alanine (H840A)

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pET-mKelly2

Plasmid

#119783

Purpose

Expresses mKelly2 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mKelly2

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a_T7-ARG2

Plasmid

#106474

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coli

Depositor

Mikhail Shapiro

Article

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pHB-3

Plasmid

#140956

Purpose

For PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.

Depositor

Kang Zhou

Article

Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

Insert

phaCAB

Expression

Bacterial

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-hTDG

Plasmid

#70758

Purpose

bacterial expression of human TDG

Depositor

Primo Schaer

Article

Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

Insert

TDG (TDG Human)

Tags

6-HIS

Expression

Bacterial

Promoter

T7/lac operon

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CYP3A4

Plasmid

#67104

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CYP3A4 (CYP3A4 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_NAD-Snifit

Plasmid

#113658

Purpose

Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentration

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pVM013

Plasmid

#64768

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA-GFP tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Menendez-Benito et al Proc Natl Acad Sci U S A. 2013 Jan 2;110

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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His6_MBP_FXR1P

Plasmid

#198694

Purpose

Expression of human FXR1 protein in E. coli

Depositor

Simpson Joseph

Article

Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

Insert

FXR1P (FXR1 Human)

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_NADP-Snifit

Plasmid

#113913

Purpose

Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTM-NiV-L

Plasmid

#234657

Purpose

Expresses L gene of NiV Bangladesh strain

Depositor

Carolina Lopez

Article

Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

Insert

NiV L gene (L Nipah virus)

Use

In cell lines expressing bacteriophage t7 rna pol…

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTM-NiV-P

Plasmid

#234656

Purpose

Expresses P gene of NiV Bangladesh strain

Depositor

Carolina Lopez

Article

Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

Insert

NiV P gene (Nvgp2 Nipah virus)

Use

In cell lines expressing bacteriophage t7 rna pol…

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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