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We narrowed to 18,759 results for: nls

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Showing: 9881 - 9920 of 18759 results

  1. p-dCas9-JMJD2A-S288G-T289G-Hygro

    Plasmid
    #104418
    Purpose
    transient expression of dCas9-dJMJD2A fusion protein
    Depositor
    Stephan Beck
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dJMJD2A
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    S288G-T289G
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104418-plasmid-map-sequence-id-204359

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  2. p-dCas9-p300-S1396R-S1397R-Hygro

    Plasmid
    #104419
    Purpose
    transient expression of dCas9-dp300 fusion protein
    Depositor
    Stephan Beck
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dp300
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    S1396R-S1397R
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104419-plasmid-map-sequence-id-204303

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  3. p-dCas9-DNMT3a-Hygro

    Plasmid
    #104403
    Purpose
    transient expression of dCas9-DNMT3a fusion protein
    Depositor
    Stephan Beck
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNMT3a (DNMT3A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104403-plasmid-map-sequence-id-204468

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  4. p-dCas9-G9a-Hygro

    Plasmid
    #104408
    Purpose
    transient expression of dCas9-G9a fusion protein
    Depositor
    Stephan Beck
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G9a (EHMT2 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104408-plasmid-map-sequence-id-204469

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  11. pTE2690

    Plasmid
    #137717
    Purpose
    Expresses dimpLbCas12a-SunTag in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dimpLbCas12a
    Use
    CRISPR
    Tags
    NLSx3 SV40 and SunTagx10
    Expression
    Mammalian
    Mutation
    D156R, G532R, K538V, Y542R, K595R, D832A,
    Promoter
    CMV
    Available since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    137717-plasmid-map-sequence-id-274029

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  12. pET-FLAG-SpCas9-HF1-plus

    Plasmid
    #126775
    Purpose
    Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    T7
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126775-plasmid-map-sequence-id-437774

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  13. B-SpCas9-HF1

    Plasmid
    #126762
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-SpCas9-HF1
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; R661A; Q695A; Q926A; amino acids 1005-1013…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    126762-plasmid-map-sequence-id-410965

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  14. pEF:iCas9

    Plasmid
    #138267
    Purpose
    Expression of mTN3-GGS6-dCas9 (iCas9) in human.
    Depositor
    Xiao Wang
    Publication
    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mTN3-GGS6-dCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Cas9-D10A, H840A
    Promoter
    Ef1a
    Available since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138267-plasmid-map-sequence-id-273623

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  15. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

    Plasmid
    #136652
    Purpose
    Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136652-plasmid-map-sequence-id-273328

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  19. pKM510

    Plasmid
    #134227
    Purpose
    Retroviral plasmid expressing mCherry - HJURP
    Depositor
    Iain Cheeseman
    Insert
    mCherry-HJURP-LacI 1-304 (HJURP Human)
    Use
    Retroviral
    Expression
    Mammalian
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    134227-plasmid-map-sequence-id-271958

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  21. BII-gR-BtW-eSpCas9

    Plasmid
    #133358
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression
    Depositor
    Blair Madison
    Publication
    Fischer et al Am J Physiol Gastrointest Liver Physiol.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9
    Tags
    Flag-tagged eSpCas9
    Expression
    Mammalian
    Mutation
    eSpCas9 mutations
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    133358-plasmid-map-sequence-id-269429

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  23. pU6-(BsaI)_CBh-UN-Cas9

    Plasmid
    #135011
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3xFLAG and 126aa domain from HSV-1 UL12 fused to …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135011-plasmid-map-sequence-id-267594

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  24. BII-gR-PtW-eSpCas9

    Plasmid
    #133357
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression
    Depositor
    Blair Madison
    Publication
    Fischer et al Am J Physiol Gastrointest Liver Physiol.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9
    Tags
    HA-tagged eSpCas9
    Expression
    Mammalian
    Mutation
    eSpCas9 mutations
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133357-plasmid-map-sequence-id-267549

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  25. pTFAM.1.0-gDNA

    Plasmid
    #132443
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TFAM (TFAM Human)
    Use
    CRISPR
    Available since
    Dec. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132443-plasmid-map-sequence-id-266777

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  26. MTK0_003

    Plasmid
    #123925
    Purpose
    Encodes the spCas9 cassette and spCas9 gRNA GFP dropout expression cassette final destination vector as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-sgRNA destination
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123925-plasmid-map-sequence-id-250079

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  28. pAC1748

    Plasmid
    #127982
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, argB selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Publication
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Available since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127982-plasmid-map-sequence-id-254497

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  29. pCAG-mIRFP670nuc-TEV-Xph20-ETWV

    Plasmid
    #133044
    Purpose
    Expresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporter
    Depositor
    Matthieu Sainlos
    Publication
    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Xph20-ETWV
    Tags
    NLS, TEV protease + TEV cleavage site, and miRFP6…
    Expression
    Mammalian
    Mutation
    S63K
    Promoter
    CAG
    Available since
    Nov. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    133044-plasmid-map-sequence-id-261169

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  31. pCAG-T3-CjCAS9-pA

    Plasmid
    #131543
    Purpose
    Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii
    Publication
    Fujii et al Int J Mol Sci. 2017 Oct 31;18(11). pii:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CjCas9
    Use
    CRISPR
    Tags
    FLAG-SV40NLS, SV40NLS, and polyA
    Expression
    Mammalian
    Mutation
    Human codon-optimized
    Promoter
    CAG, T3
    Available since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131543-plasmid-map-sequence-id-262486

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  32. pCAG-T3-eSpCAS9-NG-pA

    Plasmid
    #131466
    Purpose
    Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii
    Publication
    Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon optimized eSpCas9-NG
    Use
    CRISPR
    Tags
    Flag, NLS, and polyA
    Expression
    Mammalian
    Available since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131466-plasmid-map-sequence-id-262445

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  33. pMM0638

    Plasmid
    #129005
    Purpose
    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2
    Depositor
    Megan McClean
    Publication
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
    Expression
    Yeast
    Available since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    129005-plasmid-map-sequence-id-261246

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  36. pFA0055

    Plasmid
    #131774
    Purpose
    Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.
    Depositor
    Frank Albert
    Publication
    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.
    Insert
    20mer CASS5a guide (gCASS5a) and 5' sgRNA
    Expression
    Bacterial and Yeast
    Available since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131774-plasmid-map-sequence-id-258731

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Showing: 9881 - 9920 of 18759 results