We narrowed to 31,507 results for: REP
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Plasmid#185546PurposeExpresses surface-localized pHluorin fused to intracellular expression of mRuby under CMV promoterDepositorInsertIgK-pHluorin-TM-mRuby
ExpressionMammalianPromoterCMVAvailable SinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(0)-T2A-3X Gal80-Hsp70
Plasmid#62951PurposeCreating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 0. Gal80 is tagged with FLAG, HA and EGFP.DepositorInsert3XGal80-FLAG/HA/EGFP-Hsp70
Available SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b MOSPD2 [282-490]
Plasmid#186469PurposeExpression of human MOSPD2 (MSP domain from residue 282 to 490), fused to a 6HIS tag, in bacteriaDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
Tags6HISExpressionBacterialMutationMSP domain [282-490]Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
INS-2A-luciferase-2A-CD19-GFP
Plasmid#159349PurposeHuman INS targeting vector for knockin of 2A separated luciferase, humand CD19, and GFP. Targeted cells will be puromycin resistant.DepositorInsertsUseIns locus knockin donor vectorAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac EF1a-eGFP-Puro U6 PPP2CA g2
Plasmid#170823PurposePiggyBac Cas13d sgRNA plasmid for PPP2CA knockdownDepositorInsertCas13d PPP2CA gRNA2
UsePiggybac transposonExpressionMammalianPromoterU6Available SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)
Plasmid#159579PurposeE. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)DepositorAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-TGFa-ScNeo
Plasmid#209895PurposeTo monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertTGFa-ScNeo (TGFA Human)
UseLentiviralTagsGGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…Available SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTwist-FLAG-SRSF3_Full.Length_Codon.Optimized
Plasmid#172345PurposeExpression of FLAG-tagged, codon-optimized, full-length SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
TagsFLAGExpressionMammalianMutationcodon-optimizedPromoterEF-1aAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Synaptophysin-TdTomato-IRES2-OHT-Cre
Plasmid#134308PurposeExpresses Synaptophysin-TdTomato and also tamoxifen dependant Cre (OHT-Cre, aka Cre-ER)DepositorAvailable SinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-NRG1-ScNeo
Plasmid#209900PurposeTo monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6
Plasmid#192789PurposeaGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labelingDepositorInsertantiGFP nanobody minimizer
TagsH6 and ybbRExpressionBacterialAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-RaichuEV-Rac1(Y40C)
Plasmid#164903PurposeExpresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.DepositorInsertRaichuEV-Rac1 (RAC1 Human)
ExpressionMammalianMutationY40C mutation in the Rac1 domain whcih make the s…Available SinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQ54-VC
Plasmid#175060Purposeexpresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ54) in mammalian cellsDepositorInsertpolyQ54 (HTT Human)
TagsmVenus + mCherryExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQ25-VC
Plasmid#175062Purposeexpresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ25) in mammalian cellsDepositorInsertpolyQ25 (HTT Human)
TagsmVenus + mCherryExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS1
Plasmid#170852PurposeAAV backbone for transgene expression in neurons under the control of the hSyn1 promoter.DepositorTypeEmpty backboneUseAAVExpressionMammalianAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_HyPer7RgDAAO
Plasmid#217746PurposeDonor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.DepositorInsertHyPer7 D amino acid oxidase
TagsNuclear export signalExpressionMammalianMutationFused DAAO to the C-terminus of HyPer7 using a Gl…Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-ORAI1-EC-HA
Plasmid#174335Purposeencodes hORAI1 with HA-tag in its extracellular loop 2 and an N-terminal GFP fusion (GFP-ORAI1-EC-HA).DepositorInserthuman Orai1 (ORAI1 Human)
TagsGFP in the N-terminus and HA tag in the extracell…ExpressionMammalianPromoterCMVAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLOC-MCOLN1
Plasmid#131583PurposeFor expression of human MCOLN1 in mammalian cells, GFP-taggedDepositorAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-EGFP
Plasmid#166140PurposeEGFP expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.DepositorInsertEGFP
ExpressionYeastPromoterGAL1Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-mCherry-GJA1-20k-N1
Plasmid#49861PurposeEncodes C-terminal fragment of human connexin 43 initiating at M213 with a V5 C-terminal fusion tagDepositorInsertGJA1-20k (GJA1 Human)
TagsmCherryExpressionMammalianMutationGJA1-20k corresponds to residues 213 to 382 to Co…PromoterCMVAvailable SinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only