164,223 results

1. EGFPC1-huNFATc1EE-WT

   Plasmid

   #24219

   Depositor

   Jerry Crabtree

   Article

   Beals et al Genes Dev. 1997 Apr 1. 11(7):824-34.

   Insert

   NFATc1 (NFATC1 Human)
   

   Use

   Tags

   EE and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBSV2_PflaB-mCherryBb

   Plasmid

   #118243

   Purpose

   Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCherry

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Insert

   flagellin promoter driven mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. rAAV2/MG1.1

   Plasmid

   #184540

   Purpose

   AAV packaging plasmid, expressing Rep2 and AAV-MG1.1 capsid

   Depositor

   Minmin Luo

   Article

   Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

   Insert

   AAV-MG1.1 capsid
   

   Use

   AAV; Helper vector for packaging

   Tags

   Expression

   Mutation

   changes on AAV9 capsid: K449R, A587L, Q588M; inse…

   Promoter

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGL2-2xCLEAR-luciferase

   Plasmid

   #81120

   Purpose

   Reporter activity of the CLEAR network

   Depositor

   Sung Hee Baek

   Article

   Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

   Insert

   2xCLEAR
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   Promoter

   2xCLEAR

   Available Since

   Dec. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 LIC cloning vector (6A)

   Plasmid

   #30124

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLPCX mito Grx1-roGFP2

   Plasmid

   #64977

   Purpose

   Mammalian expression of mitochondrial Grx1-roGFP2 (retroviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Retroviral

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   mitochondrial targeting sequence

   Promoter

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1136- Desmoplakin-GFP

   Plasmid

   #32227

   Depositor

   Kathleen Green

   Article

   Godsel et al J Cell Biol. 2005 Dec 19;171(6):1045-59.

   Insert

   Desmoplakin (DSP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-GfaABC1D-GRAB_ATP1.0

   Plasmid

   #167579

   Purpose

   Express the ATP sensor GRAB_ATP1.0 in astrocytes

   Depositor

   Yulong Li

   Article

   Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

   Insert

   ATP sensor GRAB_ATP1.0 (P2RY1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   GfaABC1D

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPaint-NanoLuc

   Plasmid

   #67178

   Purpose

   CRISPaint universal donor for NanoLuc fusion tagging

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   NanoLuc
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. FASTHDR-Cterm-HaloTag-Puromycin

    Plasmid

    #167208

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUb PspCas13b + PspCas13b guide RNA

    Plasmid

    #176305

    Purpose

    Expresses PspCas13b and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    PspCas13b
    

    Use

    Tags

    HA tag

    Expression

    Insect

    Mutation

    E113K, N265S- please depositor comments

    Promoter

    Ubi-p63e

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM33_AP2B1_adaptin

    Plasmid

    #178467

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    AP2B1_adaptin (AP2B1 Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMDH

    Plasmid

    #133129

    Purpose

    A recombinant plasmid expressing mdh (malate dehydrogenase) gene

    Depositor

    Gregg Beckham

    Article

    Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    malate dehydrogenase gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    malate dehydrogenase promoter

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-TK-Firefly-Control

    Plasmid

    #122277

    Purpose

    Lentiviral vector for stable expression of Firefly luciferase.

    Depositor

    Demián Cazalla

    Article

    Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

    Insert

    Firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Thymidine kinase

    Available Since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Halo-Rab6A

    Plasmid

    #190171

    Purpose

    Expresses HaloTag-Rab6A in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    HaloTag-Rab6A
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-SpCas9(H840A)-T2A-EGFP

    Plasmid

    #221231

    Purpose

    Express nCas9 nickase (H840A) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(H840A)-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mCD8-GFP in pUAST

    Plasmid

    #17746

    Depositor

    Liqun Luo

    Article

    Lee et al Neuron. 1999 Mar . 22(3):451-61.

    Insert

    mouse CD8 (Cd8a Mouse)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLib6.4

    Plasmid

    #133783

    Purpose

    Library backbone

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV6-AC 4-μHOM

    Plasmid

    #113619

    Purpose

    Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    4-μHOM variant of EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJMP3664

    Plasmid

    #222350

    Purpose

    Expression vector with PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

    Depositor

    Jason Peters

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Prig-3::CD4::spGFP1-10

    Plasmid

    #65829

    Purpose

    For membrane contact

    Depositor

    Kang Shen, Cori Bargmann

    Article

    Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

    Insert

    Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)
    

    Use

    Tags

    spGFP1-10

    Expression

    Worm

    Mutation

    Promoter

    Prig-3

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 5'UTR CGG 99x FMR1-EGFP

    Plasmid

    #63091

    Purpose

    Contains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with EGFP.

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHygR2

    Plasmid

    #200071

    Purpose

    hygR selection nls-mScarlet marked rapid RMCE integration vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHAGE-GFP

    Plasmid

    #106281

    Purpose

    A version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.

    Depositor

    Jay Shendure

    Article

    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

    Insert

    CMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GST-RH▲-MNase

    Plasmid

    #136292

    Purpose

    Expression of catalytic inactive RNase H fused to micrococcal nuclease

    Depositor

    Kavitha Sarma

    Article

    Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

    Insert

    RNase HI-D10R-E48R and MNase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. qTAG-C-3xHA-Puro

    Plasmid

    #207729

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

    Plasmid

    #86457

    Purpose

    Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

    Depositor

    Ralf Kuehn

    Article

    Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    BFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pgk

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. plix403-V5-N-mEGFP

    Plasmid

    #223957

    Purpose

    Gateway cloning of genes of interest with an N-terminal V5-mEGFP fusion; generation of stable cells with doxycycline inducible expression

    Depositor

    Roko Žaja, Karla Feijs

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5-mEGFP

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Prig-3::CD4::spGFP11

    Plasmid

    #65830

    Purpose

    For membrane contact

    Depositor

    Kang Shen, Cori Bargmann

    Article

    Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

    Insert

    Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)
    

    Use

    Tags

    spGFP11

    Expression

    Worm

    Mutation

    Promoter

    Prig-3

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-HA-CDK4

    Plasmid

    #172612

    Purpose

    Expresses HA-tagged CDK4 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mCardinal-N1

    Plasmid

    #54590

    Purpose

    Localization: N1 Cloning Vector, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Type

    Empty backbone

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pHTN-HaloTag_BCL11A:1-773

    Plasmid

    #211014

    Purpose

    Mammalian expression of full-length human BCL11A. N-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    BCL11A:M1-F773 (BCL11A Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    isoform 2

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. AAV pCAG-FLEX-EGFP-WPRE

    Plasmid

    #51502

    Purpose

    Can be used to generate AAV virus that will express EGFP in the presence of Cre

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mRFP-Ub

    Plasmid

    #11935

    Depositor

    Nico Dantuma

    Article

    Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

    Insert

    Ubiquitin (RBX1 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-FKBP-EGFP-HOTag3

    Plasmid

    #106924

    Purpose

    Expresses FKBP-EGFP-HOTag3 in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    FKBP-EGFP-HOTag3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CMV-mEGFP-CREB

    Plasmid

    #137001

    Purpose

    mEGFP-CREB donor for G-CREB

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-EGFP-RhoA-Q63L

    Plasmid

    #12968

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    RhoA constitutively active (RHOA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q63L Constitutively active

    Promoter

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. EndophilinA1-mRuby2

    Plasmid

    #171945

    Purpose

    Expresses Endophilin A1 in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Endophilin A1 (SH3GL2 Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1-EBFP2(1-10)

    Plasmid

    #165141

    Purpose

    Expresses EBFP2(1-10) in mammalian cells

    Depositor

    Daichi Kamiyama

    Article

    Tamura et al Commun Biol. 2021 Feb 26;4(1):257. doi:

    Insert

    EBFP2(1-10)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 8, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. GFP-FBXL11

    Plasmid

    #126543

    Purpose

    Expresses GFP-tagged FBXL11 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    FBXL11 (KDM2A Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available Since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge