We narrowed to 1,601 results for: tef

1. pcDNA5/FRT/TO/intron/GFP

   Plasmid

   #113547

   Purpose

   Mammalian expression plasmid that can be used with the Flp-In T-REx system.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   GFP
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIVT-EGFP-mouseCENP-C

   Plasmid

   #174444

   Purpose

   in vitro transcription template for mouse CENP-C (N-ter EGFP tagged)

   Depositor

   Michael Lampson

   Article

   Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

   Insert

   Centromere protein C
   

   Use

   In vitro transcription template

   Tags

   EGFP

   Promoter

   T7 SP6

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCol2 flu CIITA (P#294)

   Plasmid

   #14651

   Depositor

   Matija Peterlin

   Article

   Kanazawa et al Immunity. 2000 Jan . 12(1):61-70.

   Insert

   CIITA (CIITA Human)
   

   Tags

   flu

   Expression

   Mammalian

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

4. THI VP64 pGAP T5

   Plasmid

   #161492

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2

   Depositor

   Diethard Mattanovich, Brigitte Gasser

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting T5 in the THI11 promoter)
   

   Expression

   Yeast

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. THI VP64 pGAP T1

   Plasmid

   #161488

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2

   Depositor

   Diethard Mattanovich, Brigitte Gasser

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting T1 in the THI11 promoter)
   

   Expression

   Yeast

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5/FRT/TO/intron/FlagCter

   Plasmid

   #113548

   Purpose

   Mammalian expression plasmid that can be used with the Flp-In T-REx system.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   Flag
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJAID5VY

   Plasmid

   #165048

   Purpose

   Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
   

   Expression

   Yeast

   Mutation

   OsTIR1 is codon-optimised for expression in S. ce…

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAN1935

   Plasmid

   #127203

   Purpose

   Constitutive expression of Venus-degronSwitchA and mScarlet-degronSwitchC

   Depositor

   Hana El-Samad

   Article

   Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

   Insert

   pTEF1-Venus-degronSwitch_A_t12-tENO2-pTEF2-mScarlet-Linker-degronSwitch_C-tPGK1
   

   Use

   Synthetic Biology

   Available Since

   May 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV Lex VP16 (F442A) HA (P#1710)

   Plasmid

   #14595

   Depositor

   Matija Peterlin

   Article

   Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

   Insert

   LexA
   

   Tags

   HA and VP16 (F442A)

   Expression

   Mammalian

   Mutation

   The VP16 sequence contains an F442A mutation. The…

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pEF myc Gal cycT2b (P#1719)

    Plasmid

    #14600

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

    Insert

    cycT2 (CCNT2 Human)
    

    Tags

    Gal and myc

    Expression

    Mammalian

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pET29b_GST-Nb32

    Plasmid

    #228895

    Purpose

    Express GST-tagged anti-Zika Nb32 in E.coli

    Depositor

    Magnus Rueping

    Article

    Yuli et al ACS Synthetic Biology Article ASAP

    Insert

    GST-tagged Nb32
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET29b_GST-NbD6

    Plasmid

    #228896

    Purpose

    Express GST-tagged anti-Zika NbD6 in E.coli

    Depositor

    Magnus Rueping

    Article

    Yuli et al ACS Synthetic Biology Article ASAP

    Insert

    GST-tagged NbD6
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETM11-6xHis-TEV-hORF1p_R261A

    Plasmid

    #202586

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Arginine 261 to Alanine

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pETM11-6xHis-TEV-hORF1p_StammerAEA

    Plasmid

    #202588

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Methionine 91 to Alanine, changed O…

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIVT-EGFP-ratCENP-C

    Plasmid

    #174445

    Purpose

    in vitro transcription template for rat CENP-C (N-ter EGFP tagged)

    Depositor

    Michael Lampson

    Article

    Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

    Insert

    Centromere protein C
    

    Use

    In vitro transcription template

    Tags

    EGFP

    Promoter

    T7 SP6

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIVT-EGFP-pumilioCENP-C

    Plasmid

    #174446

    Purpose

    in vitro transcription template for R. pumilio CENP-C (N-ter EGFP tagged)

    Depositor

    Michael Lampson

    Article

    Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

    Insert

    Centromere protein C
    

    Use

    In vitro transcription template

    Tags

    EGFP

    Promoter

    T7 SP6

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJAID5WY

    Plasmid

    #165050

    Purpose

    Integrative expression of SOD1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>SOD1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Expression

    Yeast

    Mutation

    OsTIR1 is codon-optimised for expression in S. ce…

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. THI VP64 pALD4 T4

    Plasmid

    #161485

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T4_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (targeting T4 of pTHI11 promoter)
    

    Expression

    Yeast

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. THI VP64 pALD4 T5

    Plasmid

    #161486

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T5_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (targeting T5 of pTHI11 promoter)
    

    Expression

    Yeast

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. THI VP64 pGAP T2

    Plasmid

    #161489

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T2_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T2 in the THI11 promoter)
    

    Expression

    Yeast

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge