We narrowed to 18,759 results for: nls
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Plasmid#121950PurposeE. coli expression, protein purificationDepositorInsertAkCas12b
Tags3xHA tag and 10xHis tagExpressionBacterialAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR
Plasmid#100008PurposeExpresses Cas9-Nlux and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into genomeDepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsCas9-NluxPromoterRibi promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR-P2A-BlastR
Plasmid#98147PurposeExpresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.DepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsP2A-BlastR fusion with Cas9-NluxPromoterRibi promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaLZ_P2A_Hygro_Barcode
Plasmid#120509PurposeBarcoded lentiviral vector to express MYC deltaLZ in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltaLZ (MYC Human)
UseLentiviralMutationDeletion of leucine zipper motifPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaHLH_P2A_Hygro_Barcode
Plasmid#120508PurposeBarcoded lentiviral vector to express MYC deltaHLH in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltaHLH (MYC Human)
UseLentiviralMutationDeletion of helix-loop-helix motifPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltab_P2A_Hygro_Barcode
Plasmid#120507PurposeBarcoded lentiviral vector to express MYC deltab in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
1518_pAAV-U6-SA-eGFP-gRNA-HLP-SACas9-HA-OLLAS-spA
Plasmid#109316PurposePlasmid for liver-specific expression of AAV SaCas9 with a gRNA against eGFPDepositorInserteGFP gRNA
UseAAV and CRISPRExpressionMammalianAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1822_pCR8-dCasRx
Plasmid#118656PurposeGateway Donor; dCasRx ORF; No promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCasRx
UseCRISPRExpressionMammalianPromoterNo promoterAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1825_pCR8-dCasRx-RBM38
Plasmid#118662PurposeGateway Donor; dCasRx-RBM38 ORF; No promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCasRx-RBM38
UseCRISPRExpressionMammalianPromoterNo promoterAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1809_pmax-RBM38-FRB
Plasmid#118642PurposeTransient transfection; Expresses RBM38-FRB; CAGGS promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRBM38-FRB
UseCRISPRExpressionMammalianPromoterCAGGSAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pV1329
Plasmid#111438PurposeSolo vector pV1326 +sgCgADE2DepositorInsertCaCas9/sgCgADE2
UseCRISPRExpressionYeastAvailable sinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA
Plasmid#113688PurposeSaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.DepositorInsertSaCas9 (NEWENTRY )
UseAAV and CRISPRTagsFlag, HAx2, KASH, and NLSPromoterCytomegalo Virus(CMV)Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJEP312-pAAV-CMV-SaCas9-P2A-HAFLAGHA-KASH-pA
Plasmid#113689PurposeSaCas9 driven by CMV. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACSDepositorInsertSaCas9 (NEWENTRY )
UseAAV and CRISPRTagsFlag, HAx2, KASH, and NLSPromoterCytomegalo Virus(CMV)Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGS-pac-T2A-iCre
Plasmid#113849PurposePuromycin resistance gene and iCre recombinaseDepositorInsertpac-T2A-iCre
ExpressionMammalianAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTF1.1.0-gDNA
Plasmid#113797PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MTF1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMTF1 (MTF1 Human)
UseCRISPRExpressionMammalianAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTFAP2B.1.0-gDNA
Plasmid#113791PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFAP2BDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTFAP2B (TFAP2B Human)
UseCRISPRExpressionMammalianAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pV1386
Plasmid#111445PurposeSolo vector pV1382 + sgScADE2DepositorInsertCaCas9/sgScADE2/stop-codon repair
UseCRISPRExpressionYeastAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHEY1.1.0-gDNA
Plasmid#112393PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HEY1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHEY1 (HEY1 Human)
UseCRISPRExpressionMammalianAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0013
Plasmid#117549PurposeLevel 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0013
Plasmid#117521PurposeLevel0 Golden Gate part: CDS, SpCas9-pDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9-p (with stop codon)
UseCRISPRAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
BCJJ180
Plasmid#117516PurposeCas9_4 Golden Gate Level 0DepositorInsertBsaI:AATG:Cas9_4:GCTT:BsaI
UseCRISPRTags3xFLAG and NLSExpressionPlantAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa VP64 Neurog2
Plasmid#99695PurposeExpresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAVDepositorPublicationInsertdCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Mouse, Synthetic)
UseAAV and CRISPRTagsVP64Mutationdead Cas9Available sinceSept. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL3 sgRNA(MS2)-MS2-AID3f
Plasmid#112129Purposeempty sgRNA cloning vector with MS2-AID3fDepositorInsertAIDmono 3f mutant (AICDA Human)
UseCRISPRExpressionMammalianMutationN- mutated and C- terminal truncated, F115Y/C116F…PromoterhU6,CBhAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL2 sgRNA(MS2)-MS2-AID3c
Plasmid#112128Purposeempty sgRNA cloning vector with MS2-AID3cDepositorInsertAID mono 3c mutant (AICDA Human)
UseCRISPRExpressionMammalianMutationN- mutated and C- terminal truncated, F115Y/C116F…PromoterhU6,CBhAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJY-RpABE-FT_INF
Plasmid#112874Purposebinary vector for INF (Induced Neo-Functionalization) of FT in ArabidopsisDepositorInsertsRPS5A promoter
ABE7.10
U6-sgRNA targeting FT
UseCRISPRExpressionPlantAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLacI-GFP-HEC1 WT
Plasmid#114049PurposeExpression of exogenous LacI-GFP-HEC1 WTDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP-HEC1 (NDC80 Human)
ExpressionMammalianMutationWT HEC1 sequenceAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST-APIC-LexAGAD
Plasmid#112807Purposegateway cloning destination vector to express enhancer driven LexAGAD in DrosophilaDepositorTypeEmpty backboneExpressionBacterialAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pV1464
Plasmid#111440PurposeN. castellii Solo CRISPR vector, marked with URA3 and NatRDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RVR
Plasmid#108861PurposeLbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutationsDepositorInsertLbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations)
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationG548R, K554V and Y558R, Cpf1 is rice codon optimi…Available sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ239-RR
Plasmid#108862PurposeFnCpf1 Gateway entry plasmid with N623R and K687R mutationsDepositorInsertFnCpf1-RR (FnCpf1 with N623R and K687R mutations)
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationN623R and K687R, Cpf1 is rice codon optimizedAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORFE0005
Plasmid#112073PurposeAtDeadCAS9 D10A H840A without stop codon module CDS1nsDepositorInsertAtDeadCAS9 D10A H840A without stop codon
UseCRISPRExpressionPlantMutationPlant codon optimized dCas9 D10A S. pyogenesAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_RAF1_94
Plasmid#111826PurposeKnockout Raf1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA targeting RAF1 94-114 (RAF1 Human)
UseCRISPRTagsmCherryExpressionBacterial and MammalianPromoterU6Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG417
Plasmid#105341PurposeYeast two hybridDepositorTypeEmpty backboneExpressionYeastAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGM4723:Ble:Cas9
Plasmid#112208PurposeGolden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9
SheBle
UseCRISPR; Diatom, phaeodactylumTagsPhaeodactylum fcp promoter and terminator and YFPMutationPhaeodactylum Fcp promoterPromoterPhaeodactylum FcpAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR sgSLC19A1
Plasmid#102314Purposegenetic depletion of SLC19A1DepositorAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-dCas9VPP300-T2A-GFP-shP53
Plasmid#102896PurposeEBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9VPP300-T2A-GFP-shP53
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACT4-DM
Plasmid#111233Purposeyeast expression vectorDepositorInsertccdB-cassette
ExpressionYeastAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3RA-PGK-Puro
Plasmid#110843PurposeLentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)DepositorInsertBE3RA
UseLentiviralMutationD10APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
20XUAS IVS Jaws mCherry_tr
Plasmid#111549PurposeOptogenetics in Drosophila: UAS-induced expression of Jaws mCherryDepositorInsertJaws_mCherry_trafficked
ExpressionInsectMutationwtPromoterhsp70Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
10XUAS IVS ChrimsonR mCherry_tr
Plasmid#111547PurposeOptogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherryDepositorInsertChrimsonR_mCherry_trafficked
ExpressionInsectMutationwtPromoterhsp70Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by