We narrowed to 30,432 results for: uros

1. CMV-mCh-kCAAX

   Plasmid

   #112267

   Purpose

   ITRACK Acceptor Construct

   Depositor

   Ryohei Yasuda

   Article

   Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

   Insert

   mCherry with KRas membrane targeting domain
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAG-mBrn2

   Plasmid

   #19711

   Depositor

   Connie Cepko

   Article

   Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

   Insert

   mouse Brn2 (Pou3f2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-T3G-CERP2

   Plasmid

   #175498

   Purpose

   All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRE-EGFP

   Plasmid

   #89871

   Purpose

   EGFP reporter vector for iTango system

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   tetO-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CWB-EGFP

   Plasmid

   #61462

   Purpose

   AAV vector with CaMKIIa promoter, EGFP, WPRE, and bGHpA

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   Feb. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

   Plasmid

   #218710

   Purpose

   AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter

   Depositor

   Bart Nieuwenhuis

   Inserts

   hSaCas9
   Chimeric guide for SaCas9
   

   Use

   AAV and CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUHD-AID

   Plasmid

   #121066

   Purpose

   vector expresses AID-fusion protein under TRE promoter and with puromycin resistance selection

   Depositor

   Randy Poon

   Article

   Ng et al Cell Cycle. 2018 Dec 23. doi: 10.1080/15

   Type

   Empty backbone

   Use

   Tags

   AID

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. SOX2se-CPG-TV

   Plasmid

   #70155

   Purpose

   a targeting vector to target the endogenous Sox2 SE CPG island with Snrp-Tomato-polyA

   Depositor

   Rudolf Jaenisch

   Article

   Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

   Insert

   5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se
   

   Use

   Mouse Targeting and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-CAG-FLEX-mCherry

   Plasmid

   #178583

   Purpose

   Cre-dependent expression of mCherry under the constitutive promoter

   Depositor

   Chun-Li Zhang

   Article

   Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-N1-jGCaMP7b-XC

    Plasmid

    #178361

    Purpose

    Improved genetic encoded calcium sensor based on GCaMP7b.

    Depositor

    Xiaodong Liu

    Article

    Geng et al eLife. 2022 Oct 5;11:e76691. doi: 10.755

    Insert

    jGCaMP7b-XC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AP-SNAP25

    Plasmid

    #167995

    Purpose

    positive selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    SNAP25-T7PAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CMV-2mCh-PS

    Plasmid

    #112268

    Purpose

    IDOCKS Acceptor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    two copies of mCherry followed by a pseudosubstrate peptide for PKC
    

    Use

    Tags

    mCh

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS2+Paxillin-mKate

    Plasmid

    #105974

    Purpose

    in vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesions

    Depositor

    Caren Norden

    Article

    Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

    Insert

    Paxillin
    

    Use

    Tags

    mKate

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-hGFAP-mCherry-shLuci

    Plasmid

    #178588

    Purpose

    Expression of mCherry and a shRNA against luciferase under the human GFAP promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    miRE-Luciferase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCLX-UBI-mCherry

    Plasmid

    #114275

    Purpose

    Ubiquitous expression of the mCherry living color

    Depositor

    Patrick Salmon

    Article

    Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-EF1a-V5-LIC

    Plasmid

    #120247

    Purpose

    For LIC cloning with N-terminal V5 tag

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCI-NLS-BirA-2A-mCherry

    Plasmid

    #127781

    Purpose

    BirA construct in a PCI nls backbone driving mCherry activity

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    BirA
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. AP-VAMP4

    Plasmid

    #168000

    Purpose

    positive selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    VAMP4(I75-L91)-T7PAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV2-GCaMP5g-P2A-PAmCherry

    Plasmid

    #133411

    Purpose

    Fluorescent reporter for calcium imaging and photoactivation.

    Depositor

    Tim Holy

    Article

    Lee et al Science. 2019 Dec 13;366(6471):1384-1389

    Insert

    GCaMP5g-P2A-PAmCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. H6-auxilin

    Plasmid

    #62571

    Purpose

    6xHis-tagged full-length bovine brain auxilin (auxilin 1) in pQE30 for protein expression in bacteria

    Depositor

    Ernst Ungewickell

    Article

    Schroder et al Eur J Biochem. 1995 Mar 1;228(2):297-304

    Insert

    Auxilin
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    *see comment below

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

21. Cenp-B INCENP GFP

    Plasmid

    #45238

    Purpose

    Target INCENP to centromeres via full length CENP-B

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    Cenp-B, INCENP-Δcen, GFP
    

    Use

    Tags

    GFP and full length CENP-B

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-Myc-rSyntenin1

    Plasmid

    #89435

    Purpose

    mammalian expression of rat Syntenin1

    Depositor

    Fritz Rathjen

    Article

    Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

    Insert

    SDCBP
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LMAPPW

    Plasmid

    #205657

    Purpose

    shRNAmir backbone for RNA interference, with Puromycin selection

    Depositor

    Dapeng Wang

    Article

    Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

    Insert

    miR-AB
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mCMV

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMAL358

    Plasmid

    #169147

    Purpose

    Encodes OptoQ-AMP1

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMAL513

    Plasmid

    #169153

    Purpose

    Encodes OptoQ-INVRT1

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCS2 hSmad1-EVE-MM

    Plasmid

    #22994

    Depositor

    Edward De Robertis

    Article

    Fuentealba et al Cell. 2007 Nov 30. 131(5):980-93.

    Insert

    Smad1 (SMAD1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S214A, S206A, S195A, S187A, S463E, S465E

    Promoter

    Available since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

27. Tol2-krt4-RFP

    Plasmid

    #97149

    Purpose

    As a control of miRNA sponge

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Type

    Empty backbone

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMUH_unc84_tdTFlag

    Plasmid

    #46024

    Depositor

    Sean Eddy

    Article

    Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

    Insert

    UNC-84 (unc-84 )
    

    Use

    10xuas-phi31-expression vector

    Tags

    tdTomato

    Expression

    Mutation

    Two amino acid mutations (F279L and L500P) compar…

    Promoter

    10XUAS

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AAV-shRNA-Tet2

    Plasmid

    #85743

    Purpose

    shRNA against mouse Tet2

    Depositor

    Hongjun Song

    Article

    Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

    Insert

    shRNA Tet2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV-sgTel

    Plasmid

    #122659

    Purpose

    Expresses sgRNA for telomere repeats with fluorescent indicator BFP and puromycin selection marker

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. cSPBN-4GFP

    Plasmid

    #52487

    Purpose

    Expresses EGFP

    Depositor

    Sebastian Seung

    Article

    Wickersham et al Nat Protoc. 2010 Mar;5(3):595-606. doi:

    Insert

    EGFP
    

    Use

    Rabies virus

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. MAPT_Halo_C_allele

    Plasmid

    #178143

    Purpose

    Donor vector for endogenous tagging of human MAPT at the C-terminus with halotag

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Halotag (MAPT Human)
    

    Use

    Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV_CMV::hTfR-GFP

    Plasmid

    #199714

    Purpose

    Expresses a chimera of human transferrin receptor (hTfR) and GFP in mammalian cells.

    Depositor

    Alice Ting

    Article

    Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

    Insert

    hTfR-GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pENTR - CaSR-EGFP

    Plasmid

    #191503

    Purpose

    A zebrafish CaSR-EGFP construct used for Gateway cloning.

    Depositor

    Michael Granato

    Article

    Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

    Insert

    CaSR-EGFP-SV40
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJEP228-Lenti-CMV-GFP-GluN2B-WPRE-pA

    Plasmid

    #84277

    Purpose

    pLenti vector backbone designed to express GFP tagged GluN2B from a CMV promoter.

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    GluN2B
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJEP227-Lenti-CMV-GFP-GluN2A-WPRE-pA

    Plasmid

    #84276

    Purpose

    pLenti vector backbone designed to express GFP tagged GluN2A from a CMV promoter.

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    GluN2A
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AAV-shRNA-Tet1

    Plasmid

    #85742

    Purpose

    shRNA against mouse Tet1

    Depositor

    Hongjun Song

    Article

    Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

    Insert

    shRNA Tet1
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCZGY3163

    Plasmid

    #101248

    Purpose

    GFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.

    Depositor

    Yishi Jin

    Article

    Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

    Insert

    gfp11::rps-18 (rps-18 Synthetic, Nematode)
    

    Use

    Gateway

    Tags

    GFP11

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AAV-CAG-LSL-mCherry-shLuci

    Plasmid

    #178590

    Purpose

    Cre-dependent expression of mCherry and a shRNA against luciferase under the constitutive promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    miRE-Luciferase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LacI INCENP

    Plasmid

    #45236

    Purpose

    Target INCENP to lac operator sequence

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    LacI, INCENP-Δcen, mCherry (lacI )
    

    Use

    Tags

    LacI (lac repressor protein) and mCherry

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    t7

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge