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pCaggs-mGold2s
Plasmid#231763PurposeExpression of mGold2t in mammalian cellsDepositorInsertmGold2s
UseTagsExpressionMammalianMutationmGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…PromoterActin promoter with CMV enhancerAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGKCMV)-nucTomato-hygro
Plasmid#208573PurposeLV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmnucTomato
d2nucEGFP
HygroR
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterPGK/CMV and minPAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Flag-SKP1-T131A
Plasmid#213734PurposeExpresses human SKP1 T131A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-T131A (SKP1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-T131A
Plasmid#213482PurposeExpresses human SKP1 T131A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-T131A (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-T131E
Plasmid#213601PurposeExpresses human SKP1 T131E mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-T131E (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsAGO1_F
Plasmid#146314PurposeMammalian Expression of HsAGO1DepositorInsertHsAGO1 (AGO1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-ShadowY
Plasmid#193557PurposeExpresses mTurquoise2-ShadowY in mammalian cells.DepositorInsertmTurquoise2-ShadowY
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-MERTK-Fc(DAPA)-AviTag-6xHis
Plasmid#156878PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertMERTK (MERTK Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCK013
Plasmid#105345PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK016
Plasmid#105348PurposeBacterial translocation for proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK014
Plasmid#105346PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCsRNAP
Plasmid#182746PurposeSwapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmidsDepositorInsertsmall RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR V5-RNF25_IRES-mCherry
Plasmid#198386PurposeRNF25 expressionDepositorInsertRNF25 (RNF25 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1AAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-flag YAP2 5SA
Plasmid#27371DepositorInsertYAP2 (YAP1 Human)
UseTagsflagExpressionMammalianMutationSerines at positions 61, 109, 127, 128, 131, 163,…PromoterAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSEED-mScarlet3:ALFA
Plasmid#219641PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in mScarlet3:ALFA using the SEED/Harvest methodDepositorInsertmScarlet:ALFA SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB-Rex
Plasmid#122134PurposeB. subtilis Rex sensor plasmid with gentamicin resistance and pBBR1 originDepositorInsertB. subtilis Rex
UseTagsExpressionBacterialMutationPromoterapFAB338Available sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Green Glifon50
Plasmid#126206Purposegreen fluorescent glucose sensor for mammalian cellsDepositorInsertGreen Glifon50
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lac-I-SceI-Tet
Plasmid#17655DepositorInsertlac I-SceI tet
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTara:500
Plasmid#60717PurposeExpresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORFDepositorInsertT7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgRNA-hSyn-mCherry
Plasmid#87916PurposesgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)DepositorInsertmCherry
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterhSynAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pInt-ERCreER
Plasmid#190040PurposeThis is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).DepositorInsertERT2-Cre-ERT2 flanked by AAVS1
UseCRISPR and TALENTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-LUC2CP/ARE
Plasmid#62857PurposeSplicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.DepositorInsertLuciferase
UseLuciferaseTagsExpressionMammalianMutationdestabilizing sequences (DS) added to the C termi…PromoterCMVAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-Ku80
Plasmid#46958PurposePlasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.DepositorInsertKu80 (XRCC5 Human)
UseTagsGFP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB533-44B12m23-sfGFP
Plasmid#186778PurposeRNAP2 Ser5ph-mintbody expression vector for mammalian cellsDepositorInsert44B12m23-scFv
UseTagssfGFPExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
optoAKT
Plasmid#207164PurposeLight-inducible expression of optoAKT; for precise temporal control of the AKT signaling pathwayDepositorInsertCRY2-mCherry-AKT(_PH)-2A-CIBN2-GFP-CAAX
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
METTL7A pEGFP-N2 (890)
Plasmid#62017Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertMETTL7A (METTL7A Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pLentiPGK Puro DEST ERKKTRClover
Plasmid#90227PurposeLentiviral vector to express ERK KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertERK Kinase Translocation Reporter
UseLentiviralTagsmCloverExpressionMammalianMutationPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCP20T-tdTomato
Plasmid#78469PurposeBroad range bacterial fluorescence labelingDepositorInserttdTomato
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only
