We narrowed to 30,686 results for: PLE

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pMP117

Plasmid

#207920

Purpose

GreenBraid destination vector pGBZ2 - PaqCI - Eco31I - spisPink- Eco31I - PaqCI

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEYFP-p23

Plasmid

#108225

Purpose

Mammalian expression vector for EYFP fusion to human p23

Depositor

Didier Picard

Article

Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

Insert

EYFP-p23 (PTGES3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pJZC588

Plasmid

#62315

Purpose

sgRNA with 2x MS2 (wt+f6) for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 2x MS2 (wt+f6) binding module

Expression

Yeast

Promoter

SNR52

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNT3/Cre

Plasmid

#89654

Purpose

Expresses a non-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

non-targeting gRNA

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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SPOON/ pcDNA3

Plasmid

#124840

Purpose

mammalian expression of SPOON, a spontaneously blinking green fluorescent protein

Depositor

Takeharu Nagai

Article

Arai et al ACS Chem Biol. 2018 Aug 17;13(8):1938-19

Insert

SPOON

Tags

No tag

Expression

Mammalian

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Ago4

Plasmid

#21536

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago4 (AGO4 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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FnR9-10

Plasmid

#182567

Purpose

Human fibronectin fragment, type-III repeats 9-10, R1379A mutant

Depositor

Timothy Springer

Article

Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

Insert

Human fibronectin fragment, type-III repeats 9-10

Tags

His and TEV cleavage site after His tag

Expression

Bacterial

Mutation

R1379A

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pJAT62

Plasmid

#204301

Purpose

Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

ie1-EGFP

Use

CRISPR

Promoter

ie1

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR/pSM2(CMV) (w57-1)

Plasmid

#17389

Purpose

Gateway entry vector for miRNA expression under CMV promoter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

RNAi; Entry vector

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)

Plasmid

#55221

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

StrepII and msfGFP

Expression

Insect

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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H6-auxilin

Plasmid

#62571

Purpose

6xHis-tagged full-length bovine brain auxilin (auxilin 1) in pQE30 for protein expression in bacteria

Depositor

Ernst Ungewickell

Article

Schroder et al Eur J Biochem. 1995 Mar 1;228(2):297-304

Insert

Auxilin

Tags

6XHis

Expression

Bacterial

Mutation

*see comment below

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
hSDH5

Plasmid

#113046

Purpose

Expression of WT hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5 (SDHAF2 Human)

Expression

Bacterial

Mutation

Insert begins at amino acid 39

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pJH643

Plasmid

#172168

Purpose

Reporter plasmid for pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para sfGFP.3TAG; Plpp pylS; PproK pylT; AraC; Cam

Expression

Bacterial

Mutation

sfGFP(N39*, N135*, Y151*)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ145-ZmUbi-tRNA

Plasmid

#158403

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAs

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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PB-T3G-CERP2

Plasmid

#175498

Purpose

All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR4 TNRC6C

Plasmid

#20023

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Trinucleotide repeat containing 6C (TNRC6C Human)

Use

Entry vector

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pEXP5-NT/pT7 LasR

Plasmid

#193629

Purpose

Constitutive T7 RNAP-mediated expression of LasR transcription factor for Las quorum sensing system. LasR sequence is codon optimized for E. coli.

Depositor

Dora Tang

Article

Gonzales et al ChemSystemsChem 2023

Insert

LasR

Tags

6xHis

Expression

Bacterial

Promoter

T7 RNAP promoter

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pWS5390-CRISPRai-URA3

Plasmid

#182829

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - URA3 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR1A-FKBP12DD C-term (w350-2)

Plasmid

#17413

Purpose

Gateway entry vector for Degron, C-term

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

FKBP12DD (L106P)

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-Alpha-synuclein-E46K

Plasmid

#181723

Purpose

Galactose inducible expression of Alpha-synuclein-E46K

Depositor

Alejandro Chavez

Insert

Alpha-synuclein (SNCA Human)

Expression

Yeast

Mutation

E46K

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR1A-NTAP-A (w322-1)

Plasmid

#17402

Purpose

Gateway entry vector for NTAP (N-terminal affinity purification), frame A

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

CBP and SBP

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pSK-Sasg

Plasmid

#214350

Purpose

Expresses cloning backbone for SlugCas9 sgRNA

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugCas9 tracrRNA

Expression

Mammalian

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huBoCas12a

Plasmid

#174673

Purpose

Expresses 6xHis-MBP tagged BoCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huBoCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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CFP-FKBP

Plasmid

#162433

Purpose

for use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

CFP-FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pJZC522

Plasmid

#62279

Purpose

MCP-VP64 fusion

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

MCP-VP64

Tags

VP64

Expression

Yeast

Promoter

pAdh

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-ald*-alaE

Plasmid

#134939

Purpose

Low phosphate inducible alanine dehydrogenase and alanine exporter

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

alanine dehydrogenase and L-alanine exporter

Expression

Bacterial

Promoter

yibD

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pJAT43

Plasmid

#204299

Purpose

For introducing heat shock inducble B3 recombinase. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

ie1-EGFP

Use

CRISPR

Promoter

ie1

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSBDEST.B

Plasmid

#79460

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Blasticidin selection

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLX302 LacZ-V5 puro

Plasmid

#98579

Purpose

Lentiviral vector for constitutive expression of human LacZ with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

LacZ

Use

Lentiviral

Tags

2x V5 tag

Expression

Mammalian

Available Since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pQCXIH-CDKN2A

Plasmid

#37104

Depositor

Joseph Nevins

Article

Angus et al Oncogene. 2012 Jan 16. doi: 10.1038/onc.

Insert

CDKN2A (CDKN2A Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only