8,969 results

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pAAV-Ef1a-DIO hChR2(C128S/D156A)-mCherry

Plasmid

#35504

Purpose

AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2(C128S/D156A)-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

C128S and D156A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-ERK2

Plasmid

#37145

Depositor

Rony Seger

Article

Rubinfeld et al J Biol Chem. 1999 Oct 22;274(43):30349-5

Insert

ERK2 (MAPK1 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

aa1-6 deleted as a result of cloning strategy

Promoter

CMV

Available Since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
Flag FoxO3

Plasmid

#14937

Depositor

Joan Massague

Article

Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

Insert

FoxO3 (FOXO3 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pOPINM

Plasmid

#26044

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

His-MBP-3C-lacZ

Tags

MAHHHHHHSSG_MBP_LEVLFQGP

Expression

Bacterial, Insect, and Mamm…

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pB18cmBFPAzurite

Plasmid

#14034

Depositor

Patrick Daugherty

Article

Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

Insert

Azurite

Use

Yeast/bacteria optimized codons

Tags

6xHis

Expression

Bacterial

Available Since

Feb. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pENTR-AIF

Plasmid

#16182

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Apoptosis-inducing factor (AIFM1 Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

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Tagged-Cap

Plasmid

#35572

Depositor

Marian Waterman

Article

Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

Type

Empty backbone

Use

Luciferase

Tags

Ms2 Stem Loop

Expression

Mammalian

Promoter

SV40

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3 IKKE K38A flag

Plasmid

#26202

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

I-kappa-B kinase epsilon K38A (IKBKE Human)

Tags

Flag

Expression

Mammalian

Mutation

Catalytic Lysine changed to Alanine (K38A)

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-14-3-3 zeta GST

Plasmid

#13278

Depositor

Michael Yaffe

Article

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 zeta (YWHAZ Bovine)

Tags

GST

Expression

Bacterial

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only
CMV-d2eGFP-empty

Plasmid

#26164

Depositor

Phil Sharp

Article

Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

Insert

Expression

Mammalian

Mutation

…ccgcCTCGAG[sponge sites]GGGCCCgttt… became …ccgc…

Available Since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Atg5

Plasmid

#24922

Depositor

Toren Finkel

Article

Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

Insert

Atg5 (ATG5 Human)

Tags

Myc

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pGEX5X.1 PINK1 WT

Plasmid

#13321

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 kinase domain (PINK1 Human)

Tags

GST

Expression

Bacterial

Mutation

Amino acids 112-496

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only

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6-His-MBP-TEV-FnCpf1

Plasmid

#90094

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 (humanized)

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET YBX-1

Plasmid

#13035

Depositor

Peter Newburger

Article

Shen et al J Biol Chem. 1998 Mar 6. 273(10):5443-6.

Insert

YBX1 (YBX1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only
FLAG-SENP6

Plasmid

#18065

Depositor

Edward Yeh

Article

Dou et al Mol Cell. 2010 Aug 13. 39(3):333-45.

Insert

SENP6 (SENP6 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Jnk3a2

Plasmid

#13759

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a2 (MAPK10 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pRB1017

Plasmid

#59936

Purpose

empty vector for gRNA cloning

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Type

Empty backbone

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Hygro HA Akt2

Plasmid

#16000

Depositor

Morris Birnbaum

Insert

Akt2 (AKT2 Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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Duet011

Plasmid

#17627

Depositor

Linzhao Cheng

Article

Zhou et al Stem Cells. 2007 Mar . 25(3):779-89.

Insert

UBC promoter - GFP - PGK promoter - Hygro

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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FLAG-Foxo1(pCMV5)

Plasmid

#12148

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 (Foxo1 Mouse)

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2007

Availability

Academic Institutions and Nonprofits only

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DRH334: FCK-QuasAr2-mO2

Plasmid

#51692

Purpose

Fluorescent reporter of membrane voltage with improved sensitivity

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr2-mOrange

Use

Lentiviral

Tags

mOrange2

Expression

Mammalian

Promoter

CaMKIIa

Available Since

April 25, 2014

Availability

Academic Institutions and Nonprofits only

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pFLAG-XBP1u-FLuc

Plasmid

#31239

Depositor

Jimtong Horng

Article

Jheng et al Cell Microbiol. 2010 Jun . 12(6):796-813

Insert

FLAG-XBP1u-FLuc (XBP1 Human)

Tags

Flag and Fluc

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-S5E2-Gq-P2A-dTomato

Plasmid

#135635

Purpose

AAV vector to drive the expression of Gq-DREADD-P2A-dTomato in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, and AAV9

Insert

Gq-P2A-dTomato

Use

AAV

Mutation

None

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pECE HA AMPKa1 WT

Plasmid

#69504

Purpose

Expresses AMPK alpha1 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCMVHA E2F2

Plasmid

#24226

Purpose

Mammalian expression of human E2F2 with HA tag

Depositor

Kristian Helin

Article

Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

Insert

E2F2 (E2F2 Human)

Tags

HA

Expression

Mammalian

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.CaMPARI.WPRE.SV40

Plasmid

#100832

Purpose

AAV expression of CaMPARI, a photoconvertible fluorescent protein-based calcium integrator

Depositor

Loren Looger, Eric Schreiter

Article

Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

Has Service

AAV1, AAV5, and AAV9

Insert

CaMPARI

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pST-KT

Plasmid

#44561

Purpose

for ectopic inducible expression of mycobacterial proteins

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Promoter

UV15 promoter

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CaMPARI V398D

Plasmid

#60786

Purpose

Expresses CaMPARI V398D in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

Depositor

Loren Looger, Eric Schreiter

Article

Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

Insert

CaMPARI V398D

Tags

Nuclear Export Signal

Expression

Mammalian

Mutation

V398D

Promoter

CMV

Available Since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-FLEX-Arch-GFP

Plasmid

#22222

Purpose

Cre-dependent viral expression of Arch-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Has Service

AAV1 and AAV9

Insert

Arch

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

no

Promoter

CAG

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-Blasticidin-CAG-Flpe-ERT2

Plasmid

#68461

Purpose

AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

mammalian-codon-optimized Flpe recombinase fused with ERT2

Use

Synthetic Biology

Tags

ERT2

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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8,969 results