19,646 results

1. Plenti-ARE-HSVTK-luc

   Plasmid

   #161789

   Purpose

   Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers

   Depositor

   Hans-Guido Wendel

   Article

   Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi

   Inserts

   Luciferase-T2A-HSV-TK
   mCherry P2A blasticidin
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   EF1a and Murine Gclc ARE

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSFFV_mNG3A(1-10)

   Plasmid

   #157992

   Purpose

   Lentiviral vector of constitutive expression of mNeonGreen3A(1-10)

   Depositor

   Bo Huang

   Article

   Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

   Insert

   pSFFV_mNG3K(1-10)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   W148L, M150V, K153M, N194K, F204L

   Promoter

   SFFV

   Available Since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKOV

   Plasmid

   #25769

   Depositor

   George Church

   Article

   Link et al J Bacteriol. 1997 Oct . 179(20):6228-37.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Aug. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. MBP-ch-TOG-His6

   Plasmid

   #69108

   Purpose

   For bacterial expression of recombinant human ch-TOG tagged with MBP and His.

   Depositor

   Stephen Royle

   Article

   Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

   Insert

   Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Available Since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-EB3mCherry_cmv-LifeActGFP

   Plasmid

   #133927

   Purpose

   Live dual labeling of the actin network and polymerizing microtubules for dynamic analysis of the cytoskeleton

   Depositor

   Frank Bradke, Sebastián Dupraz

   Article

   Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

   Insert

   EB3-mCherry and Lifeact-GFP (MAPRE3 Human)
   

   Expression

   Mammalian

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCE048-SiT-Cas12a

   Plasmid

   #128124

   Purpose

   Express both AsCas12a and CRISPR arrays on single transcripts

   Depositor

   Randall Platt

   Article

   Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

   Insert

   AsCas12a-Triplex
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRU42

   Plasmid

   #167664

   Purpose

   ENTRY vector for gRNA2 cloning under pAtU3 promoter

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pAtU3
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Yes-EGFP

   Plasmid

   #110497

   Purpose

   Fluorescent Yes

   Depositor

   Bernardo Mainou

   Insert

   Yes (YES1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKanCMV-mClover3-mRuby3

   Plasmid

   #74252

   Purpose

   Mammalian expression of tandem fusion of mClover3-mRuby3

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Insert

   mClover3-mRuby3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-Tubulin-C-18

    Plasmid

    #55148

    Purpose

    Localization: Microtubules, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

    Insert

    Tubulin (TUBA1B Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-EML4-ALK variant 1

    Plasmid

    #183828

    Purpose

    Express EML4-ALK fusion variant 1 (E13;A20) in mammalian cells

    Depositor

    Aaron Hata

    Article

    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-13 and ALK exon…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. mVenus-pBAD

    Plasmid

    #54845

    Purpose

    Localization: Protein Expression Vector , Excitation: 515, Emission: 528

    Depositor

    Michael Davidson, Atsushi Miyawaki

    Article

    Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

    Type

    Empty backbone

    Tags

    6xHis and mVenus

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. cfSGFP2-col4a1

    Plasmid

    #160317

    Purpose

    cfSGFP2-tagged collagen4a1 for mammalian expression

    Depositor

    Ikuo Wada

    Article

    Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

    Insert

    COL4A1 (COL4A1 Human)
    

    Tags

    cfSGFP2

    Expression

    Mammalian

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVP91A-pcaHG

    Plasmid

    #113766

    Purpose

    Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.

    Depositor

    John Lipscomb

    Article

    Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11

    Inserts

    β subunit of Protocatechuate 3,4- Dioxygenase
    α subunit of Protocatechuate 3,4- Dioxygenase
    

    Tags

    Met-6xHis

    Expression

    Bacterial

    Promoter

    T5 promoter

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCh-Sec61 beta

    Plasmid

    #49155

    Purpose

    expression of Sec61 beta fused to mCherry, used as a general ER marker

    Depositor

    Gia Voeltz

    Article

    Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

    Insert

    Sec61 beta (SEC61B Human)
    

    Tags

    mCherry*

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. PGK-AAVS1ZFNR

    Plasmid

    #60915

    Purpose

    encodes zinc finger nuclease specific to AAVS1 locus (right)

    Depositor

    Paul Gadue

    Article

    Sim et al Methods Mol Biol. 2015 Jan 29.

    Insert

    AAVS1-ZFNR
    

    Promoter

    PGK

    Available Since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET22b RNase A

    Plasmid

    #58903

    Purpose

    Bacterial expression of bovine pancreatic ribonuclease A

    Depositor

    Ronald Raines

    Article

    delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

    Insert

    RNase A (RNASE1 Bovine)
    

    Tags

    pelB sequence

    Expression

    Bacterial

    Mutation

    See Depositor Comments below

    Promoter

    T7

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3-U6-sgRNA-EGFP

    Plasmid

    #107721

    Purpose

    For expression of sgRNA from the U6 promoter with EGFP expression simultaneously

    Depositor

    Xingxu Huang

    Article

    Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSIN4-EF2-ABCG2-IRES-Neo

    Plasmid

    #25983

    Depositor

    Ren-he Xu

    Article

    Zeng et al Stem Cells. 2009 Oct . 27(10):2435-45.

    Insert

    ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2 (ABCG2 Human)
    

    Use

    Lentiviral

    Tags

    EF1-apha promoter and IRES-Neo

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Ef1a-fDIO EYFP

    Plasmid

    #55641

    Purpose

    Flp-Dependent EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EYFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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