159,352 results

1. AnkyrinG-190-GFP

   Plasmid

   #31059

   Depositor

   Vann Bennett

   Article

   Mohler et al J Biol Chem. 2002 Mar 22. 277(12):10599-

   Insert

   Ankyrin G (Ank3 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PB-NES-ZapCV2

   Plasmid

   #222950

   Purpose

   Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

   Depositor

   Amy Palmer

   Article

   Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

   Insert

   NES-ZapCV2 (NES Synthetic)
   

   Use

   Tags

   ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…

   Expression

   Mammalian

   Mutation

   ECFP gene is truncated 36 bp at 3' end. Venu…

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMSCV-IRES-GFP-MD2-HA

   Plasmid

   #133065

   Purpose

   Retroviral expression of mouse MD2 tagged with HA

   Depositor

   Jonathan Kagan

   Article

   Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

   Insert

   MD2 (Ly96 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDR196

   Plasmid

   #36029

   Depositor

   Dominique Loque, Doris Rentsch, Wolf Frommer

   Article

   Meyer et al J Biol Chem. 2006 Mar 17;281(11):7197-20

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBabe-Puro-Myr-Flag-IKBKE

   Plasmid

   #15293

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   IKBKE (IKBKE Human)
   

   Use

   Retroviral

   Tags

   Myr-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pSupAR-MbPylRS(DiZPK)

   Plasmid

   #91705

   Purpose

   express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest

   Depositor

   P. Chen

   Article

   Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d

   Inserts

   pylS
   pylT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

   Promoter

   Available since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

   Plasmid

   #185914

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRiA4-VIR

   Plasmid

   #138193

   Purpose

   Virulence helper compatible with pCambia binary vectors.

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   vir gene cluster
   

   Use

   Vir gene expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PS-CFP2-Farnesyl-5

   Plasmid

   #57227

   Purpose

   Localization: Membrane, Excitation: 400 / 490, Emission: 468 / 511

   Depositor

   Michael Davidson

   Insert

   Farnesyl (HRAS Human)
   

   Use

   Tags

   PS-CFP2

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. medSIIN

    Plasmid

    #185664

    Purpose

    shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

    Inserts

    mScarlet
    miR-30a
    shApc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. lenti-SpCas9 puro

    Plasmid

    #104994

    Purpose

    This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TRPC3-Myc

    Plasmid

    #25902

    Depositor

    Craig Montell

    Article

    Li et al Neuron. 1999 Sep . 24(1):261-73.

    Insert

    TRPC3 (Trpc3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PS-CFP2-Clathrin-15

    Plasmid

    #57220

    Purpose

    Localization: Clathrin Vesicles, Excitation: 400 / 490, Emission: 468 / 511

    Depositor

    Michael Davidson

    Insert

    Clathrin (CLTB Human)
    

    Use

    Tags

    PS-CFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. phOCT4-Luc

    Plasmid

    #17221

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

    Insert

    Upstream region of human OCT4 (POU5F1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Red Glifon 300

    Plasmid

    #163115

    Purpose

    Red fluorescent protein-based glucose sensor for mammalian cells with higer affinity

    Depositor

    Takashi Tsuboi

    Article

    Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

    Insert

    Red Glifon 300
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMK289 (mAID-mClover-NeoR)

    Plasmid

    #72827

    Purpose

    C-terminal tagging with mAID-mClover using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mClover-Neo
    

    Use

    CRISPR

    Tags

    mAID-mClover

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 5HRE/GFP

    Plasmid

    #46926

    Purpose

    hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system

    Depositor

    Thomas Foster, Martin Brown

    Article

    Vordermark et al Neoplasia. 2001 Nov-Dec;3(6):527-34.

    Insert

    5X HRE of VEGF (VEGFA Human)
    

    Use

    Tags

    d2EGFP

    Expression

    Mammalian

    Mutation

    five copies of a 35-bp fragment from the hypoxia-…

    Promoter

    EF-1α promoter

    Available since

    Aug. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p415-PtrpC-Cas9-TtrpC-CYC1t

    Plasmid

    #68059

    Purpose

    Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    humanized Cas9
    

    Use

    CRISPR; N. crassa, fungi

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP-NR2A

    Plasmid

    #17924

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    NMDAR2A (Grin2a Rat)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-Myc CDC4 WT*

    Plasmid

    #16652

    Depositor

    Bert Vogelstein

    Article

    Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

    Insert

    CDC4 (FBXW7 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    *Note that this insert is only WT relative to the…

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV.CAG.GCaMP6f.WPRE.SV40

    Plasmid

    #100836

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV2, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    CAG

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)

    Plasmid

    #38262

    Depositor

    Robert Lefkowitz

    Article

    Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

    Insert

    β-arrestin2 (Arrb2 Rat)
    

    Use

    Tags

    Flag and PIP5K Iα core kinase domain (residues 18…

    Expression

    Mammalian

    Mutation

    Arg 233, Arg237, and Lys251 converted to Glutamin…

    Promoter

    CMV

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

23. GST-TAX1BP1

    Plasmid

    #171423

    Purpose

    For the purifcation of TAX1BP1 protein

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    TAX1BP1 (TAX1BP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PB-GFP11x7-HygR

    Plasmid

    #223676

    Purpose

    To check the split GFP signals in mitochondria (2)

    Depositor

    Juan Melero-Martin

    Article

    Lin et al Nature. 2024 May;629(8012):660-668. doi:

    Insert

    GFP11x7
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pU6-BbsI-chiRNA

    Plasmid

    #45946

    Purpose

    Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

    Depositor

    Kate O'Connor-Giles, Melissa Harrison, Jill Wildonger

    Article

    Gratz et al Genetics. 2013 May 24.

    Insert

    U6-BbsI-chiRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Dm-snRNA:U6:96Ab

    Available since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MSCV Luciferase PGK-hygro

    Plasmid

    #18782

    Depositor

    Scott Lowe

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLPC-scribP305L

    Plasmid

    #24601

    Depositor

    Senthil Muthuswamy

    Article

    Zhan et al Cell. 2008 Nov 28. 135(5):865-78.

    Insert

    Scribble (SCRIB Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    P305L

    Promoter

    Available since

    May 5, 2010

    Availability

    Academic Institutions and Nonprofits only

28. tdEos-CDC42-C-10

    Plasmid

    #57596

    Purpose

    Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Insert

    CDC42 (CDC42 Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

29. pB-mTSPAN4-GFP

    Plasmid

    #210329

    Purpose

    Overexpression of mTSPAN4

    Depositor

    Li Yu

    Article

    Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

    Insert

    mTSPAN4 (Tspan4 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLX-EF1a_Empty

    Plasmid

    #221480

    Purpose

    Empty vector control for pLX307 overexpresion experiments

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. ET8-GPIba-high-6His

    Plasmid

    #180251

    Purpose

    High affinity mutant of human platelet membrane protein GP1b alpha

    Depositor

    Timothy Springer

    Article

    Jiang et al J Mol Biol. 2019 Mar 29;431(7):1380-1396

    Insert

    human GPIbalpha 1-290aa with mutation G233V and M239V (GP1BA Human)
    

    Use

    Tags

    6His

    Expression

    Mammalian

    Mutation

    G233V and M239V

    Promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLV-REPORT(PGK/CMV)

    Plasmid

    #172330

    Purpose

    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    PGK/CMV

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGEX SH3(2)-4R

    Plasmid

    #112090

    Purpose

    Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3(2)-4R (NCK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUC19-mAirn-R-loop

    Plasmid

    #134900

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    mAirn promoter (Airn Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTOPO-MCL1

    Plasmid

    #21605

    Depositor

    Ulrich Maurer

    Article

    Maurer et al Mol Cell. 2006 Mar 17. 21(6):749-60.

    Insert

    MCL-1 (MCL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Insert ends with a STOP codon, no V5-His-tag.

    Promoter

    Available since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

36. 1245 pCMVb p300

    Plasmid

    #10717

    Depositor

    William Sellers

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMaCTag-P13

    Plasmid

    #120024

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mCherry
    

    Use

    Pcr template

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. KRphi-mRFP

    Plasmid

    #17276

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2006 Jul 21. 313(5785):347-51.

    Insert

    KR phi
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2008

    Availability

    Academic Institutions and Nonprofits only

39. W118-1_hTGM2-V5

    Plasmid

    #180413

    Purpose

    lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal V5 tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM2 (TGM2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLib6.4B-Aalb_743

    Plasmid

    #176676

    Purpose

    Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge