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We narrowed to 24,354 results for: CRISPR

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Showing: 5081 - 5100 of 24354 results

  1. pZNF408-donor

    Plasmid
    #112323
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF408 with GFP
    Depositor
    Kevin White
    Insert
    ZNF408 homology arms flanking EGFP-IRES-Neo cassette (ZNF408 Human)
    Use
    CRISPR
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112323-plasmid-map-sequence-id-228535

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  2. pZNF398-donor

    Plasmid
    #112319
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF398 with GFP
    Depositor
    Kevin White
    Insert
    ZNF398 homology arms flanking EGFP-IRES-Neo cassette (ZNF398 Human)
    Use
    CRISPR
    Mutation
    Unknown
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112319-plasmid-map-sequence-id-228524

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  3. pZNF70-donor

    Plasmid
    #112318
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF70 with GFP
    Depositor
    Kevin White
    Insert
    ZNF70 homology arms flanking EGFP-IRES-Neo cassette (ZNF70 Human)
    Use
    CRISPR
    Mutation
    Unknown
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112318-plasmid-map-sequence-id-228523

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  4. pZNF266-donor

    Plasmid
    #112306
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF266 with GFP
    Depositor
    Kevin White
    Insert
    ZNF266 homology arms flanking EGFP-IRES-Neo cassette (ZNF266 Human)
    Use
    CRISPR
    Mutation
    Unknown
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112306-plasmid-map-sequence-id-228458

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  5. pZSCAN32-donor

    Plasmid
    #112304
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF434 with GFP
    Depositor
    Kevin White
    Insert
    ZNF434 homology arms flanking EGFP-IRES-Neo cassette (ZSCAN32 Human)
    Use
    CRISPR
    Available Since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112304-plasmid-map-sequence-id-228421

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  6. pZNF165-donor

    Plasmid
    #112302
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF165 with GFP
    Depositor
    Kevin White
    Insert
    ZNF165 homology arms flanking EGFP-IRES-Neo cassette (ZNF165 Human)
    Use
    CRISPR
    Mutation
    Unknown
    Available Since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112302-plasmid-map-sequence-id-228414

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  11. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid
    #139999
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139999-plasmid-map-sequence-id-346119

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  12. lenti dCAS-VP64_Blast

    Plasmid
    #61425
    Purpose
    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Lentiviral Prep
    Insert
    dCAS9(D10A, N863A)-VP64_2A_Blast
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1A
    Available Since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    61425-plasmid-map-sequence-id-457486

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  13. lenti sgRNA(MS2)_puro backbone

    Plasmid
    #73795
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73795-plasmid-map-sequence-id-181202

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  15. lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2

    Plasmid
    #170378
    Purpose
    Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.
    Depositor
    Jeremy Day
    Article
    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21
    Insert
    A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    human Synapsin promoter
    Available Since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    170378-plasmid-map-sequence-id-333532

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  18. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid
    #160729
    Purpose
    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    160729-plasmid-map-sequence-id-322926

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  20. pLV-dCas9-KRAB-PGK-HygR

    Plasmid
    #83890
    Purpose
    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    Use
    CRISPR and Lentiviral
    Tags
    Flag
    Mutation
    D10A and H840A
    Promoter
    Human Ubiquitin C Promoter and mouse phosphoglyce…
    Available Since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83890-plasmid-map-sequence-id-240902

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Showing: 5081 - 5100 of 24354 results