We narrowed to 7,526 results for: Ski;

1. lentiCRISPR v2-sgBAX-2

   Plasmid

   #210130

   Purpose

   knock out BAX in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

   Insert

   Apoptosis regulator BAX (BAX Human)
   

   Use

   CRISPR

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBabe_puro_DEST_Flag_FNDC3B

   Plasmid

   #45269

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   FNDC3B (FNDC3B Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pPHR-mCherry-hPMLIII

   Plasmid

   #103824

   Purpose

   Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-mCherry-hPMLIII (CRY2, PML Human, Mustard Weed)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX Hck-SH2

   Plasmid

   #46445

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Hck (HCK Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 134-254

   Promoter

   Tac

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-HDC-DIO-GFP-shRNA.scramble

   Plasmid

   #184331

   Purpose

   Expresses scramble shRNA for control expts. Flexed (DIO) cassette driven by hdc promoter fragment for pan neuronal expression

   Depositor

   William Wisden

   Article

   Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

   Insert

   hdc pan neuronal gene promoter, flex switch, GFP, scramble shRNA
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and RNAi

   Expression

   Mammalian

   Promoter

   histidine decarboxylase promoter fragment that g…

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX Stat1-SH2

   Plasmid

   #46511

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Stat1 (STAT1 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 488-683

   Promoter

   Tac

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX Lyn-SH2

   Plasmid

   #46452

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Lyn (LYN Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 106-213

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNL-CEF-IgA1

   Plasmid

   #187009

   Purpose

   Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1

   Depositor

   Hermann Eibel

   Article

   Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

   Insert

   Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1
   

   Use

   Lentiviral

   Available Since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX Bmx-SH2

   Plasmid

   #46409

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Bmx (BMX Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 286-400

   Promoter

   Tac

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. gRNA-CXCR4(90)

    Plasmid

    #98962

    Purpose

    Mammalian expression plasmid for CRISPR gRNA targeting human CXCR4 exon 2

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Encodes a gRNA for CRISPR-mediated targeting of C…

    Promoter

    U6

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1/NDUFS8/myc-His

    Plasmid

    #127489

    Purpose

    Expresses myc-tagged NDUFS8 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS8 (NDUFS8 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV NucleoBAS N354K-I372V

    Plasmid

    #63574

    Purpose

    Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

    Depositor

    Stan van de Graaf

    Article

    van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

    Insert

    NucleoBAS N354K-I372V
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    N345K and I372V

    Promoter

    CMV

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCEP4-myc-GRM3-VC

    Plasmid

    #98968

    Purpose

    myc-tagged human mGluR3 fused to C-terminus of split Venus

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    GRM3 (GRM3 Human)
    

    Tags

    Signal/leader sequence from HLA class I A-2 alpha…

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

    Plasmid

    #138525

    Purpose

    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

    Depositor

    Richard Jenner

    Article

    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

    Insert

    Control non-PRC2 binding A-tract repeat RNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXS-IRES-Blast SHMT2 CD

    Plasmid

    #106302

    Purpose

    Expresses SHMT2 K280A catalytic site mutant

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    serine hydroxymethyltransferase 2 (SHMT2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    K280A catalytic site mutation, Silent mutations d…

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV.GFAP.SF-iGluSnFR.S72A

    Plasmid

    #106194

    Purpose

    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.S72A
    

    Use

    AAV

    Mutation

    GltI: S72A

    Promoter

    GFAP

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCIBN-tagBFP-TetR

    Plasmid

    #103797

    Purpose

    BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-tagBFP-TetR (CIB1, tetR E. coli, Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    TetR: A4G (M2V)

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. psiCHECK2-E2F1 3'UTR

    Plasmid

    #29468

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    E2F1 3'UTR (E2F1 Human)
    

    Expression

    Mammalian

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

19. psiCHECK2-p27Kip1 3'UTR

    Plasmid

    #29476

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    p27 Kip1 3'UTR (CDKN1B Human)
    

    Expression

    Mammalian

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

20. mCherry-FKBP-TPD54(R159E)

    Plasmid

    #172453

    Purpose

    Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag R159E mutant

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD54 (TPD52L2 Human)
    

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Mutation

    changed Arginine 159 to Glutamic Acid

    Promoter

    CMV

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge