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pDIRECT_22C

Plasmid

#91135

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers

Use

CRISPR

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-AsCas12f1

Plasmid

#171614

Purpose

AsCas12f1-based Human cell genome editing

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

AsCas12f1 and sgRNA_v1

Expression

Mammalian

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCFD5

Plasmid

#73914

Purpose

multiplex gRNA plasmid

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

dU6-3:tRNA:gRNA:tRNA:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-AML1/ETO-IRES-GFP

Plasmid

#60832

Purpose

AML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFP

Depositor

Scott Lowe

Article

Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

Insert

AML1/ETO (RUNX1, RUNX1T1 Human)

Use

Retroviral

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-QUASM1:mScarletNLS-SV40pA

Plasmid

#155124

Purpose

Tol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:mScarletNLS

Use

Zebrafish expression

Promoter

QUASM1-E1b

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-SRm160

Plasmid

#11305

Depositor

Phil Sharp

Article

Cheng et al Mol Cell Biol. 2006 Jan . 26(1):362-70.

Insert

SRm160 (SRRM1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pKDC.015

Plasmid

#184992

Purpose

Express -Eco2 RT and ncRNA in human cells

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco2: RT and ncRNA(wt), a1/a2 length: 13

Expression

Mammalian

Mutation

Human codon optimized RT

Promoter

TetOn-3g

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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AktPH-mCherry-T2A-PHR-T2A-CIBNcaax

Plasmid

#173861

Purpose

Mammalian expression of Akt PH-mCherry-T2A-PHR-T2A-CIBNcaax (PIP3 marker and opto-control)

Depositor

Takao Nakata

Article

Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

Insert

AktPH-mCherry-T2A-PHR-T2A-CIBNcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 mmu-mir-208a

Plasmid

#26331

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-208a (Mir208a Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pGL421_MLL2_promoter

Plasmid

#118696

Purpose

Express MLL2/KMT2B promoter for luciferase assay

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

MLL_promoter

Use

Luciferase

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST12.2-DYRK2 (469T)

Plasmid

#174850

Purpose

Expression of DYRK2

Depositor

Helen Morrison

Insert

DYRK2 (DYRK2 Human)

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-CXCR4-W161Y

Plasmid

#98951

Purpose

Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant W161Y

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

W161Y: enhanced CXCL12 binding and signaling

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-CXCR4-W161H

Plasmid

#98952

Purpose

Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant W161H

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

W161H: enhanced CXCL12 binding and signaling

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Gamma2L/S [N452/73R]

Plasmid

#177553

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Gamma2L/S (Human). Derived from hybridoma N452/73.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Gamma2L/S (Homo sapiens) recombinant mouse monoclonal antibody (GABRG2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_eCB2.0

Plasmid

#164604

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Mutation

S383T

Promoter

hSyn

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pminiTol2-5xUAS-hsp-sfGFP-zP2A-tetoff-zN2cG-zP2A-zTVA (UGNT-A)

Plasmid

#240272

Purpose

Helper plasmid for zebrafish rabies virus based-circuit tracing

Depositor

Xufei Du

Article

Chen et al eLife 13:RP100880

Inserts

N2cG (codon-optimized for Danio rerio)
sfGFP (codon-optimized for Danio rerio)
TVA (codon-optimized for Danio rerio)
tetoff (codon-optimized for Danio rerio)

Use

Zebrafish expression

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pminiTol2-5xUAS-hsp-sfGFP-zP2A-zN2cG-zP2A-TVA (UGNT)

Plasmid

#240273

Purpose

Helper plasmid for zebrafish rabies virus based-circuit tracing

Depositor

Xufei Du

Article

Chen et al eLife 13:RP100880

Inserts

N2cG (codon-optimized for Danio rerio)
sfGFP (codon-optimized for Danio rerio)
TVA (codon-optimized for Danio rerio)

Use

Zebrafish expression

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pMC_HT7_BSDminus_F0

Plasmid

#225627

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an HaloTag7 synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Insert

HT7 and BSD

Use

CRISPR

Available Since

April 14, 2026

Availability

Academic Institutions and Nonprofits only

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pMC_HT7_BSDminus_F1

Plasmid

#225628

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an HaloTag7 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Insert

HT7 and BSD

Use

CRISPR

Available Since

April 14, 2026

Availability

Academic Institutions and Nonprofits only

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pMC_HT7_BSDminus_F2

Plasmid

#225629

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an HaloTag7 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Insert

HT7 and BSD

Use

CRISPR

Available Since

April 14, 2026

Availability

Academic Institutions and Nonprofits only

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