176,403 results

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pET His6 MBP TEV LIC cloning vector (1M)

Plasmid

#29656

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, MBP, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

47 Articles

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AAVS1-TALEN-R

Plasmid

#59026

Purpose

TALEN designed to target the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Publication

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

AAVS1-TALEN-R

Use

AAV and TALEN

Tags

3xFlag and NLS

Expression

Mammalian

Promoter

CMV

Available since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

57 Articles

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pcDNA3.1-Peredox-mCherry

Plasmid

#32383

Depositor

Gary Yellen

Publication

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry

Expression

Mammalian

Promoter

CMV

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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Tet-O-FUW-Ascl1

Plasmid

#27150

Depositor

Marius Wernig

Publication

Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

Insert

Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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pShuttle-CMV

Plasmid

#16403

Purpose

Shuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.

Depositor

Bert Vogelstein

Publication

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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p414-TEF1p-Cas9-CYC1t

Plasmid

#43802

Purpose

A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

Depositor

George Church

Publication

Dicarlo et al Nucleic Acids Res

Insert

Human Optimized S. pyogenes Cas9

Use

CRISPR

Expression

Yeast

Promoter

TEF1 promoter

Available since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

70 Articles

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SIRT3 Flag

Plasmid

#13814

Purpose

Mammalian expression of human SIRT3 with flag tag

Depositor

Eric Verdin

Publication

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT3 (SIRT3 Human)

Tags

flag

Expression

Mammalian

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pCas9-CR4

Plasmid

#62655

Purpose

Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR

Depositor

Kristala Prather

Publication

Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

Insert

Cas9 nuclease

Expression

Bacterial

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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p426-SNR52p-gRNA.CAN1.Y-SUP4t

Plasmid

#43803

Purpose

Encodes a gRNA that targets Can1.Y in yeast.

Depositor

George Church

Publication

Dicarlo et al Nucleic Acids Res

Insert

CAN1.y gRNA (CAN1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available since

March 21, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

61 Articles

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pCMV(MinDis).iGluSnFR

Plasmid

#41732

Purpose

Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Insert

GltI based Glutamate Biosensor

Use

Glutamate biosensor

Tags

Myc, PDGFR transmembrane helix (aa 513-561), and …

Expression

Mammalian

Mutation

cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

Promoter

CMV

Available since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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pRK5-HA-Ubiquitin-K33

Plasmid

#17607

Purpose

Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

Depositor

Ted Dawson

Publication

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K33 only, other lysines mutated to arginines; P19…

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pB-CAGGS-dCas9-KRAB-MeCP2

Plasmid

#110824

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

Depositor

Alejandro Chavez, George Church

Publication

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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EZ-Tet-pLKO-Blast

Plasmid

#85973

Purpose

Tet/Dox inducible shRNA lentivirus with blasticidin selection

Depositor

Cindy Miranti

Publication

Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

Type

Empty backbone

Use

Lentiviral and RNAi

Promoter

H1 / TetO

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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mEGFP-N1

Plasmid

#54767

Purpose

Localization: N1 Cloning Vector, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Type

Empty backbone

Tags

mEGFP

Expression

Mammalian

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

43 Articles

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pTRE-Tight-MitoTimer

Plasmid

#50547

Purpose

Expresses the Timer protein targeted to the mitochondria under a Tet-inducible promoter

Depositor

Roberta Gottlieb

Publication

Hernandez et al Autophagy. 2013 Nov 1;9(11):1852-61. doi

Insert

MitoTimer

Expression

Mammalian

Mutation

F12S mutation in Timer

Available since

March 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pMXs-Nanog

Plasmid

#13354

Purpose

Retroviral expression of mouse Nanog

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

Nanog homeobox (Nanog Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3.1(+)IRES GFP

Plasmid

#51406

Purpose

Expresses GFP from internal ribosome entry sequence, cloning component

Depositor

Kathleen_L Collins

Publication

Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

Insert

IRES GFP

Expression

Mammalian

Promoter

CMV

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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IKK-2 S177E S181E

Plasmid

#11105

Purpose

Mammalian expression of constitutively active human IKK-2

Depositor

Anjana Rao

Publication

Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

Insert

IKK-2 (IKBKB Human)

Tags

FLAG

Expression

Mammalian

Mutation

S177E and S181E, constitutively active

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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pENTR4-HaloTag (w876-1)

Plasmid

#29644

Depositor

Eric Campeau

Type

Empty backbone

Use

Gateway Cloning

Tags

HaloTag

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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GW1CMV-Perceval

Plasmid

#21737

Purpose

can be used to monitor the ATP:ADP ratio during live-cell imaging

Depositor

Gary Yellen

Publication

Berg et al Nat Methods. 2009 Feb;6(2):161-6. doi: 1

Insert

Perceval (fluorescent ATP:ADP sensor)

Tags

5'UTR

Expression

Mammalian

Mutation

improved Kozak sequence

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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