Addgene

We narrowed to 28,391 results for: REP

Showing: 751 - 780 of 28391 results

Results per page:

Sort results by:

pLJM1-zeo-2xStrep-BFLF1

Plasmid

#162661

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged BFLF1 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 V5-DEST mir-1-1 reporter

Plasmid

#46646

Purpose

expression of query pri-miRNA

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir-1-1 reporter

Use

RNAi; Expression of query pri-mirna sequences in …

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-Cas9-VQR-GFP_activity_reporter

Plasmid

#87156

Purpose

This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).

Depositor

Daniel Bauer

Article

Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

Insert

GFP and sgRNA targeting GFP (NGA restricted)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter mir16-1 wt

Plasmid

#46683

Purpose

Expresses wild-type miR-16-1 in mammalian cells

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir16-1 wt (MIR16-1 Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiR-report-MYB-mut

Plasmid

#141115

Purpose

Reporter plasmid of mutant MYB in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

MYB (MYB Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

A to T mutations at m6A sites

Promoter

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
1kb Zfp521 promoter reporter

Plasmid

#104188

Purpose

luciferase reporter to assay Zfp521 promoter activation

Depositor

Kathryn Hentges

Article

Yu et al Genesis. 2016 Oct;54(10):519-533. doi: 1

Insert

Zfp521 promoter - 1 Kb (Zfp521 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

none

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pISlin41w(s) (lin-41 microRNA wt reporter)

Plasmid

#11906

Depositor

David Bartel

Article

Lewis et al Cell. 2003 Dec 26. 115(7):787-98.

Insert

lin-41 miRNA target sequence (lin-41 Nematode)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
TP901 GFP BAC Reporter

Plasmid

#78216

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Use

Tags

Expression

Mutation

Promoter

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 2xFLAG-2xSTREP EMI1

Plasmid

#158947

Purpose

Express human EMI1 in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

Insert

EMI1 (FBXO5 Human)

Use

Tags

2X FLAG 2X STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUGW-GFP-2-cut reporter

Plasmid

#131669

Purpose

Lentiviral plasmid that expresses a GFP-based reporter for monitoring CRISPR based precise end-joining.

Depositor

Alberto Ciccia

Article

Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

Insert

GFP-2-cut reporter

Use

Lentiviral

Tags

P2A

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIR-REPORT-MYC CDS_WT

Plasmid

#108924

Purpose

MYC mRNA CDS region with wild type m6A motifs was inserted into pMIR-REPORT vector

Depositor

Jianjun Chen

Article

Su et al Cell. 2018 Jan 11;172(1-2):90-105.e23. d

Insert

MYC (MYC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-Nsp14-TEV-Strep

Plasmid

#154953

Purpose

Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli

Depositor

Justin Kollman

Insert

Nsp14-TEV-Strep (ORF1ab SARS-CoV-2)

Use

Tags

TEV-Strep

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pET22-Strep-TEV-Nsp10

Plasmid

#154958

Purpose

Expresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

Depositor

Justin Kollman

Insert

Strep-TEV-Nsp10 (ORF1ab SARS-CoV-2)

Use

Tags

Strep-TEV

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

July 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA4/TO-mu24-ORF24-2xStrep

Plasmid

#138453

Purpose

Expresses mu24-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

mu24-ORF24 (ORF24 MHV68/KSHV)

Use

Tags

Strep

Expression

Mammalian

Mutation

mu24 amino acids 1-191 fused to ORF24 amino acids…

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-zeo-ORF66-2xStrep

Plasmid

#130957

Purpose

Lentiviral vector for expression of KSHV ORF66 with a C-terminal 2xStrep tag

Depositor

Britt Glaunsinger

Article

Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

Insert

ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))

Use

Lentiviral

Tags

2xStrep

Expression

Mutation

Promoter

CMV

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PhiC31 GFP BAC Reporter

Plasmid

#78214

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Use

Tags

Expression

Mutation

Promoter

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxbi GFP MCP Reporter

Plasmid

#78225

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Use

Tags

Expression

Mutation

Promoter

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter mir129-2-wt

Plasmid

#46730

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir129-2-wt (MIR129-2 Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS303-pGAL-HA-SrtAstrep (pKS78)

Plasmid

#50029

Purpose

Galactose-inducible expression of cytosolic S. pyogenes SrtA in yeast

Depositor

Hidde Ploegh

Article

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

HA-SrtA(strep)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

pGAL

Available since

Jan. 26, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIR-Report-RASA1-3'UTR

Plasmid

#62575

Purpose

Translational Luciferase Reporter containing a 926 bp fragment of the RASA1 3'UTR.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

RASA1 (RASA1 Human)

Use

Luciferase

Tags

None

Expression

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.2 mir-1-1 reporter mir30-wt

Plasmid

#46695

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir30-wt (MIR30A Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter mir30-5

Plasmid

#46692

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir30-5 (MIR30A Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
REST corepressor 2_L (OZ553)

Plasmid

#28078

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting REST corepressor 2 (rcor2 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
REST corepressor 2_R (OZ554)

Plasmid

#28079

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting REST corepressor 2 (rcor2 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
pUC19_msfGFP-HP1a-repair-template

Plasmid

#238248

Purpose

For knock-in msfGFP to HP1a locus

Depositor

Denes Hnisz

Insert

HP1a

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19_msfGFP-SRRM2-repair-template

Plasmid

#238247

Purpose

For knock-in msfGFP to SRRM2 locus

Depositor

Denes Hnisz

Insert

SRRM2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIPZ-2xFLAG-2xSTREP-E2F1

Plasmid

#236433

Purpose

doxycycline inducible expression of E2F1 in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F1 (E2F1 Human)

Use

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIPZ-2xFLAG-2xSTREP-E2F2

Plasmid

#236434

Purpose

doxycycline inducible expression of E2F2 in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F2 (E2F2 Human)

Use

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIPZ-2xFLAG-2xSTREP-E2F3A

Plasmid

#236435

Purpose

doxycycline inducible expression of E2F3A in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F3A (E2f3 Mouse)

Use

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 2xFLAG-2xSTREP TFDP2

Plasmid

#236445

Purpose

transient overexpression of TFDP2 in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

TFDP2 (TFDP2 Human)

Use

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge